Application of gold nanoparticles in separation sciences

Abstract

The review article is devoted mainly to the description of applications of gold nanoparticles (GNPs) in separation sciences, especially in electromigration and chromatographic techniques. The applications of GNPs in particular separation methods, CE, microchip CE, MEKC, CEC, HPLC and GC, are classified according to the molecular size of the analytes from low-molecular-mass compounds via medium sized substances to biopolymers (proteins and nucleic acids). A very recent and promising utilization of GNPs for sample preparation, preconcentration and preseparation of selected analytes from complex matrices is presented as well. Moreover, in two introductory sections, typical preparation procedures of the GNPs and their modifications are presented and physicochemical and analytical methods employed for characterization of the native and modified GNPs are briefly introduced.

Green biosynthesis of gold nanometre scale plates using the leaf extracts from an indigenous Australian plant Eucalyptus macrocarpa

“…Metallic nanometre scale particles, in particular colloidal gold nanoparticles (Au NPs), have attracted considerable interest in many fields such as medicine, biotechnology, materials science, photonics and electronics [1][2][3][4]. The size, shape and surface morphology can have a profound influence on the chemical, physical, optical and electronic properties of nanomaterials [5,6].…”

Recent advances in column coatings for capillary electrophoresis of proteins

“…Recently used nanomaterial coatings for capillary electrophoresis separation of proteins are summarized in Table 3. CE-UV Cytochrome C, lysozyme, bovine serum albumin, myoglobin [69] Although nanoparticles applied mainly as permanent coating materials, gold nanoparticles in protein analysis by capillary electrophoresis have also been used both for permanent and dynamic coatings [70]. Yu et al used didodecyldimethylammonium bromide (DDAB) capped gold nanoparticles (AuNPs) to form a stable dynamic capillary wall coating [61] that generated 75% greater reversed EOF compared to DDAB.…”

Monitoring the On-line Concentration and Separation of Gold Nanoparticles Using the Reversed Electrode Polarity Stacking Mode and Micellar Electrokinetic Chromatography

“…(iii) The three-dimensional description of the particle size is only a reconstruction of two-dimensional images, based on the observation of a set of particles and presuming their random orientation. 13 In addition to these microscopy methods, dynamic light scattering (DLS) 14 is also employed widely to measure the sizes of metal particles. Although DLS can be used to rapidly measure the sizes of NP products, it is difficult to infer the properties of an ensemble of NPs, such as their physical properties (e.g., absorbance), using this approach.…”