Application of graphene oxide nanosheet lactate biosensors in continuous assessment of athlete fitness

Deng, Shulin

doi:10.1016/j.aej.2024.01.017

Cited by 4 publications

(1 citation statement)

References 40 publications

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“…Dagar et al [42] proposed an amperometric biosensor obtained via covalent immobilization of LOx at a nanohybrid of chitosan, iron oxide nanoparticles, and carboxylated multiwalled carbon nanotubes electrodeposited onto a Au electrode. Deng [43] designed an impedimetric biosensor combining LOx with graphene oxide nanosheets to measure L-lactate levels in sweat with real-time response, which made it possible to monitor the physiological variations in L-lactate from resting states to peak exertion levels. Fernandes et al [44] reported an amperometric biosensor based on carbon fiber microelectrodes modified with Pt nanoparticles and LOx to directly and continuously measure L-lactate levels during Status epilepticus in the rat brain (extracellular space).…”

Section: Introductionmentioning

confidence: 99%

L-Lactate Electrochemical Biosensor Based on an Integrated Supramolecular Architecture of Multiwalled Carbon Nanotubes Functionalized with Avidin and a Recombinant Biotinylated Lactate Oxidase

Tamborelli,

Mujica,

Amaranto

et al. 2024

Biosensors

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L-Lactate is an important bioanalyte in the food industry, biotechnology, and human healthcare. In this work, we report the development of a new L-lactate electrochemical biosensor based on the use of multiwalled carbon nanotubes non-covalently functionalized with avidin (MWCNT-Av) deposited at glassy carbon electrodes (GCEs) as anchoring sites for the bioaffinity-based immobilization of a new recombinant biotinylated lactate oxidase (bLOx) produced in Escherichia coli through in vivo biotinylation. The specific binding of MWCNT-Av to bLOx was characterized by amperometry, surface plasmon resonance (SPR), and electrochemical impedance spectroscopy (EIS). The amperometric detection of L-lactate was performed at −0.100 V, with a linear range between 100 and 700 µM, a detection limit of 33 µM, and a quantification limit of 100 µM. The proposed biosensor (GCE/MWCNT-Av/bLOx) showed a reproducibility of 6.0% and it was successfully used for determining L-lactate in food and enriched serum samples.

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Section: Introductionmentioning

confidence: 99%

L-Lactate Electrochemical Biosensor Based on an Integrated Supramolecular Architecture of Multiwalled Carbon Nanotubes Functionalized with Avidin and a Recombinant Biotinylated Lactate Oxidase

Tamborelli,

Mujica,

Amaranto

et al. 2024

Biosensors

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The Promise of Carbon Nano‐Onions: Preparation, Characterization and Their Application in Electrochemical Sensing

Almeida Gonzalez,

Hernandez Ojeda,

Corcho‐Valdés

et al. 2024

Analysis & Sensing

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Carbon nano‐onions (CNOs) promise to improve the range of applications of carbon materials for electroanalytical applications. In this review we explore the synthesis, characterization, and electrochemical applications of CNOs. CNOs‐based sensors present impressive features, including low detection limits in the femtogram per milliliter range, a wide linear detection range spanning up to 7 orders of magnitude, exceptional selectivity, reproducibility, and stability. Synthetic methods and characterization techniques for CNOs were thoroughly examined, shedding light on their pivotal role in biosensing technologies. Comparative analyses with other carbon materials underscore CNOs' competitive performance, either surpassing or matching many counterparts. Despite their relatively recent integration in biosensing applications, CNOs exhibit comparable or superior results concerning other carbon‐based materials. Indeed, the incorporation of CNOs into hybrid nanocomposites have shown promising outcomes, indicating a synergistic potential for future advancements in biosensing technologies. Our review provides a broad approach of the application of CNOs to the field, with emphasis in breakthroughs of the last 5 years.

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Laser-induced and catalyst-free formation of graphene materials for energy storage and sensing applications

Kumar,

Pandey,

Joanni

et al. 2024

Chemical Engineering Journal

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Application of graphene oxide nanosheet lactate biosensors in continuous assessment of athlete fitness

Cited by 4 publications

References 40 publications

L-Lactate Electrochemical Biosensor Based on an Integrated Supramolecular Architecture of Multiwalled Carbon Nanotubes Functionalized with Avidin and a Recombinant Biotinylated Lactate Oxidase

L-Lactate Electrochemical Biosensor Based on an Integrated Supramolecular Architecture of Multiwalled Carbon Nanotubes Functionalized with Avidin and a Recombinant Biotinylated Lactate Oxidase

The Promise of Carbon Nano‐Onions: Preparation, Characterization and Their Application in Electrochemical Sensing

Laser-induced and catalyst-free formation of graphene materials for energy storage and sensing applications

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