Application of high EPA-producing<i>Mortierella alpina</i>in laying hen feed for egg DHA accumulation

Tang, Xin; Chen, Haiqin; Ge, Chengfeng; Dong, Shijian; Si, Shaohong; Liu, Jiarong; Gu, Zhennan; Zhang, Hao; Chen, Yong Q.; Chen, Wei

doi:10.1039/c8ra06525j

Cited by 3 publications

(1 citation statement)

References 31 publications

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“…Several studies have been conducted to exploit M. alpina for specific fatty acid production, ,− and due to its high ARA yield (over 50% of total fatty acid content), M. alpina has already been used in food additives as well as in infant formula production. , …”

Section: Introductionmentioning

confidence: 99%

Role of Adenosine Monophosphate Deaminase during Fatty Acid Accumulation in Oleaginous Fungus Mortierella alpina

Chang

Tang

et al. 2019

J. Agric. Food Chem.

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In oleaginous micro-organisms, nitrogen limitation activates adenosine monophosphate deaminase (AMPD) and promotes lipogenesis via the inhibition of isocitrate dehydrogenase. We found that the overexpression of homologous AMPD in Mortierella alpina favored lipid synthesis over cell growth. Total fatty acid content in the recombinant strain was 15.0−34.3% higher than that in the control, even though their biomass was similar. During the early fermentation stage, the intracellular AMP level reduced by 40−60%, together with a 1.9−2.7-fold increase in citrate content compared with the control, therefore provided more precursors for fatty acid synthesis. Moreover, the decreased AMP level resulted in metabolic reprogramming, reflected by the blocked TCA cycle and reduction of amino acids, distributing more carbon to lipid synthesis pathways. By coupling the energy balance with lipogenesis, this study provides new insights into cell metabolism under nitrogen-limited conditions and targets the regulation of fatty acid accumulation in oleaginous micro-organisms.

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Section: Introductionmentioning

confidence: 99%

Role of Adenosine Monophosphate Deaminase during Fatty Acid Accumulation in Oleaginous Fungus Mortierella alpina

Chang

Tang

et al. 2019

J. Agric. Food Chem.

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Oleaginous fungi: a promising source of biofuels and nutraceuticals with enhanced lipid production strategies

Hassane,

Eldiehy,

Saha

et al. 2024

Arch Microbiol

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Advances in improving the biotechnological application of oleaginous fungus Mortierella alpina

Chang

Chen

Tang

et al. 2021

Appl Microbiol Biotechnol

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Application of high EPA-producingMortierella alpinain laying hen feed for egg DHA accumulation

Abstract: A high EPA-producing M. alpina was fermented and added to laying hen feed for egg yolk DHA accumulation.

Cited by 3 publications

References 31 publications

Role of Adenosine Monophosphate Deaminase during Fatty Acid Accumulation in Oleaginous Fungus Mortierella alpina

Role of Adenosine Monophosphate Deaminase during Fatty Acid Accumulation in Oleaginous Fungus Mortierella alpina

Oleaginous fungi: a promising source of biofuels and nutraceuticals with enhanced lipid production strategies

Advances in improving the biotechnological application of oleaginous fungus Mortierella alpina

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