Application of high‐performance liquid chromatography with photodiode array detection to the metabolic profiling of plant isoprenoids

Fraser, Paul D.; Pinto, M. E. S.; Holloway, Daniel E.; Bramley, Peter M.

doi:10.1111/j.1365-313x.2000.00896.x

Cited by 108 publications

(121 citation statements)

References 33 publications

Supporting

Mentioning

118

Contrasting

Order By: Relevance

“…Carotenoid and chlorophyll analysis. The extraction of carotenoids and chlorophylls was performed on lyophilized tissue that had been homogenized (Fraser et al, 2000). Separation and identification by Thin Layer Chromatography was performed using HPTLC silca gel 60 plates, developed in hexane:ethylacetate 60:40 (v/v) with authentic standards for reference.…”

Section: Biochemical Characterizationmentioning

confidence: 99%

“…De-esterification of ketocarotenoid esters was performed using cholesterol esterase from Pseudomonas (Sigma, UK) as described in Nogueira et al (2017). Identification was carried out by co-chromatography and comparison of spectral properties with authentic standards and reference spectra (Fraser et al, 2000). Quantitative determination of carotenoids was performed by comparison with dose-response curves (0.2-1.0 lg) constructed from authentic standards.…”

Section: Biochemical Characterizationmentioning

confidence: 99%

See 1 more Smart Citation

The road to astaxanthin production in tomato fruit reveals plastid and metabolic adaptation resulting in an unintended high lycopene genotype with delayed over‐ripening properties

Enfissi

Nogueira

D’Ambrosio³

et al. 2019

Plant Biotechnology Journal

Self Cite

View full text Add to dashboard Cite

Summary Tomato fruit are an important nutritional component of the human diet and offer potential to act as a cell factory for speciality chemicals, which are often produced by chemical synthesis. In the present study our goal was to produce competitive levels of the high value ketocarotenoid, astaxanthin, in tomato fruit. The initial stage in this process was achieved by expressing the 4, 4′ carotenoid oxygenase ( crtW ) and 3, 3′ hydroxylase ( crtZ ) from marine bacteria in tomato under constitutive control. Characterization of this genotype showed a surprising low level production of ketocarotenoids in ripe fruit but over production of lycopene (~3.5 mg/g DW ), accompanied by delayed ripening. In order to accumulate these non‐endogenous carotenoids, metabolite induced plastid differentiation was evident as well as esterification. Metabolomic and pathway based transcription studies corroborated the delayed onset of ripening. The data also revealed the importance of determining pheno/chemotype inheritance, with ketocarotenoid producing progeny displaying loss of vigour in the homozygous state but stability and robustness in the hemizygous state. To iteratively build on these data and optimize ketocarotenoid production in this genotype, a lycopene β‐cyclase was incorporated to avoid precursor limitations and a more efficient hydroxylase was introduced. These combinations resulted in the production of astaxanthin (and ketocarotenoid esters) in ripe fruit at ~3 mg/g DW . Based on previous studies, this level of product formation represents an economic competitive value in a Generally Regarded As Safe ( GRAS ) matrix that requires minimal downstream processing.

show abstract

Section: Biochemical Characterizationmentioning

confidence: 99%

Section: Biochemical Characterizationmentioning

confidence: 99%

The road to astaxanthin production in tomato fruit reveals plastid and metabolic adaptation resulting in an unintended high lycopene genotype with delayed over‐ripening properties

Enfissi

Nogueira

D’Ambrosio³

et al. 2019

Plant Biotechnology Journal

Self Cite

View full text Add to dashboard Cite

show abstract

“…Injections (20 ll) were made, and separations performed on a reverse phase (RP) C 30 5-lm column (250 · 4AE6 mm i.d.) coupled to a 20 · 4AE6 mm C 30 guard column (YMC Inc., Wilmington, NC, USA) maintained at a constant temperature of 25°C as described previously (Fraser et al 2000). The mobile phases used were methanol (solvent A), MeOH : H 2 O (80 : 20) (v ⁄ v) containing 0AE2% (w ⁄ v) ammonium acetate (solvent B) and tert-butyl methyl ether (solvent C).…”

Section: Pigment Analysismentioning

confidence: 99%

Carotenoids found inBacillus

Khaneja

Pérez-Fons

Fakhry

et al. 2009

Journal of Applied Microbiology

102

View full text Add to dashboard Cite

show abstract

“…HPLC analyses were carried out using an Agilent 1100 Series HPLC system as described by Fraser et al (2000) but slightly modified. Carotenoids were separated using a reverse-phase C30 column (5 μm, 250×4.6 mm) (YMC Inc., Wilmington, NC, USA) with mobile phases consisting of methanol (A), 0.2 % ammonium acetate aqueous solution/methanol (20/80 v/v) (B), and tert-methyl butyl ether (C).…”

Section: Hplc Analysis Of E Vitamin and Carotenoidsmentioning

confidence: 99%

Posidonia residues can be used as organic mulch and soil amendment for lettuce and tomato production

Grassi

Mastrorilli²,

Mininni

et al. 2014

Agron. Sustain. Dev.

View full text Add to dashboard Cite

There is a need for sustainable fertilizers because common mineral fertilizers are increasingly costly and often induce water and air pollution. For instance, seagrass compost could be used as fertilizer in the coastal areas of the Mediterranean, thus also solving the issue of beached residues. Here, we studied organic fertilization by application of seagrass-based compost in a tomato and lettuce crop succession.\ud Composts were made of posidonia and yard wastes at 1/4 w/w fresh weight ratio. Compost was applied at two concentrations: 10 and 20 Mg/ha fresh weight. Fresh leaves of posidonia were also tested directly as organic mulch in plots treated with conventional fertilizers. A control treatment involved mineral fertilization without mulching soil. Herbicide applications were performed in non-mulched plots immediately before transplanting of tomato and lettuce for controlling the weeds, while mulched treatments did not receive any herbicide application. Results show that 20 Mg ha−1 of posidonia-based compost is equivalent to the inorganic fertilization because the nutritional status and yield of tomato and lettuce were statistically similar. No phytotoxicity symptoms were observed in any of the tomato and lettuce plants mulched with posidonia. The same growth rate was observed for tomato and lettuce growing under conventional management practices: mineral fertilizer and herbicide applications. Overall, our findings show that seagrass-based compost is a promising, sustainable fertilizer for tomato and lettuce

show abstract

Application of high‐performance liquid chromatography with photodiode array detection to the metabolic profiling of plant isoprenoids

Cited by 108 publications

References 33 publications

The road to astaxanthin production in tomato fruit reveals plastid and metabolic adaptation resulting in an unintended high lycopene genotype with delayed over‐ripening properties

The road to astaxanthin production in tomato fruit reveals plastid and metabolic adaptation resulting in an unintended high lycopene genotype with delayed over‐ripening properties

Carotenoids found inBacillus

Posidonia residues can be used as organic mulch and soil amendment for lettuce and tomato production

Contact Info

Product

Resources

About