Application of high-speed counter-current chromatography for preparative separation of cyclic peptides from Vaccaria segetalis

Vaccaria segetalisactivates cellular lactogenesis signaling pathway of dairy cow mammary epithelial cells in vitro

“…The seed of this plant is used for the promotion of diuresis and milk secretion, activation of blood circulation and the relief of carbuncle. Some compounds were separated from Vaccaria segetalis (Qi et al 2014;Wang et al 2011), but to date the active compound has still not been known, and there are few reports on the lactogenic mechanism of Vaccaria segetalis. In our previous reports, we found that Vaccaria segetalis promotes lactogenesis by enhancing JAK-STAT5 and mTOR pathways in dairy cow mammary epithelial cells (DCMECs) Tong et al 2011), but how Vaccaria segetalis excerts its role is still unknown.…”

A sensitive and selective LC‐MS/MS method for the quantitative determination of segetalin A from the plasma of rats

“…) is a novel cyclic hexapeptide [cyclo‐(Gly–Val–Pro–Val–Trp–Ala)] and a chief cyclic peptide component of V. segetalis seeds (Morita et al ., ). According to the literature, very few analytical methods have been reported for the analysis of segetalin A in V. segetalis (Wang et al ., ; Dong et al ., ; Qi et al ., ). The first method involved a high‐speed counter‐current chromatography to separate cyclic peptides from the seeds of V. segetalis (Wang et al ., ).…”

“…Cyclic peptides are widely distributed natural products that exhibit a range of biological activities (Itokawa et al, 1997). Cyclic peptides isolated from V. segetalis have become a subject of interest because of their vasorelaxant and estrogen-like activities (Morita et al, 2006;Wang et al, 2011). A previous work reported that V. segetalis extract exerts ameliorative effects on osteopenia in ovariectomized rats (Shih et al, 2009).…”

Isolation and purification of seven lignans from Magnolia sprengeri by high-speed counter-current chromatography

“…High-speed counter-current chromatography (HSCCC) is a kind of liquid-liquid partition chromatography, which has been widely used in preparative isolation and purification of compounds from natural materials [10]. The well-known advantages of HSCCC such as no irreversible adsorption, high sample recovery and efficiency, low risk of sample denaturation, ease to scale-up and low cost were gradually confirmed by numerous reports on the isolation and purification of various lignans from natural plants effectively [10][11][12][13][14][15]. Nevertheless, there is no report on the application of HSCCC for the isolation and purification of lignans from M. sprengeri.…”