Application of host‐specific source‐tracking tools for rapid identification of fecal contamination in fresh produce by humans and livestock

Lee, Chang‐Soo; Lee, Ji-Young

doi:10.1002/jsfa.5855

Cited by 3 publications

(2 citation statements)

References 36 publications

(47 reference statements)

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“…Using FST technologies in tracking fecal pollution sources in foods has attracted increasing interest recently [73][74][75]. Pathogens carried by feces can enter the food production chain through various paths, from farm to table.…”

Section: Seafoodmentioning

confidence: 99%

“…The work of Ravaliya et al [74] in Northern Mexico has provided evidence that Bacteroidales 16S rDNA markers may serve to distinguish both general and humanfeces contamination in the production environment. Furthermore, FST can be used for the rapid identification of human or livestock fecal contamination in fresh produce [73]. It is generally believed that FST technologies can be a powerful tool to manage food safety in produce production.…”

Section: Fresh Producementioning

confidence: 99%

See 1 more Smart Citation

Host-specific Genetic Markers of Fecal Bacteria for Fecal Source Tracking in Food and Water

Zheng¹,

Shen²

2018

J Food Microbiol Saf Hyg

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Fecal pollution in food and water is the major cause of disease outbreaks, so accurate identification of this fecal pollution in these locations is critical to prevent such outbreaks. Fecal source tracking (FST) is an effective tool to identify the sources (human vs. animal) of any particular instance of fecal pollution. Many FST technologies were developed over the past two decades and used mainly to monitor and manage water quality, but their applications have recently attracted more attention in food systems. With the great advances of technologies in DNA sequencing and related bioinformatic tools, a significant number of novel genetic markers of fecal indicator bacteria (FIB) have been identified to be associated with particular host species, and thus, suitable for FST. The host-specific genetic markers were originally limited to 16S rDNA sequences of FIB, which are relatively conserved. Novel genetic markers include genes of the host-bacterium interaction and intervening sequences within the 16S-and 23S-rDNA of fecal bacteria. However, most of the genetic markers were only evaluated in laboratory settings, and their compete values in the monitoring and management of food and water safety need further assessments in the field.

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Section: Seafoodmentioning

confidence: 99%

Section: Fresh Producementioning

confidence: 99%