Application of HPLC, Gel electrophoresis and size exclusion chromatography for separation and characterization of storage proteins of soybean and peanut

Abstract

Summary. Certain practical aspects in the separation of large biomolecules have been discussed with the chosen examples of storage proteins from two important crops, viz, soybean and peanut. Importance of seeds and storage proteins along with classification and nomenclature of plant proteins is briefly mentioned. The present paper describes an updated knowledge and status on structural features of glycinin (major storage protein of soybean) and arachin (major storage protein of peanut) with respect to their molecular species, subunit structure, and their association-dissociation behavior by using techniques of high-performance liquid chromatography (HPLC), electrophoresis and size exclusion chromatography (SEC) which supplemented each other.

Role of Antihypertensive Peptides from Plant (sesame, almond and pumpkin) Seed Storage Protein

“…The two main reserve proteins of soybean that are used in the food industry are 7S β-conglycinin and 11S glycinin globulins. The 7S globulin is a trimeric glycoprotein KDa) composed of three subunits, α (57 kDa), α' (58 kDa) and β (42 kDa), associated by hydrophobic interactions (Tan-Wilson and Wilson, 2012;Marambe et al, 2012;Bhushan and Dixit, 2012;Jyothi, 2007). Sesame (Sesamum indicum) is the most ancient oil seed known and used by humans as a food source and it is mainly cultivated for its oil, whole or dehulled seeds are used in confectionery foods (Sumaya Hassan Abdel Rahim, 2015).…”