Application of <i>in vitro</i> digestibility methods in aquaculture: constraints and perspectives

Moyano, Francisco Javier; Rodrigáñez, Miguel Ángel Saénz de; Díaz, Manuel; Tacon, Albert G. J.

doi:10.1111/raq.12065

Cited by 53 publications

(70 citation statements)

References 134 publications

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“…These in vivo ADC data are considerably higher than the in vitro crude protein digestibility determined for H. illucens and T. molitor determined by Marono et al (2015), which ranged from 65.8 to 68.7%. This indicates that the in vitro method may not be adequate for estimating the ADC of protein for fish (Gomes et al 1998;Moyano et al, 2015). Digestibility of HM was also determined for turbot, showing low/moderate digestibility for organic matter, crude protein, crude lipid, and gross energy (Kroeckel et al, 2012).…”

Section: Accepted Manuscriptmentioning

confidence: 99%

Black soldier fly (Hermetia illucens) pre-pupae meal as a fish meal replacement in diets for European seabass (Dicentrarchus labrax)

et al. 2017

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Section: Accepted Manuscriptmentioning

confidence: 99%

Black soldier fly (Hermetia illucens) pre-pupae meal as a fish meal replacement in diets for European seabass (Dicentrarchus labrax)

et al. 2017

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“…The pH-stat in vitro method has been widely used in the evaluation of the nutritional quality of raw materials and processed feeds, because it is simple, fast, and with high reproducibility (Moyano et al, 2014). The use of pHstat system has proved to be suitable for checking the quality of the protein sources, for marine shrimp feed formulation (Ezquerra et al, 1998;Lemos et al, 2004Lemos et al, , 2009.…”

Section: Introductionmentioning

confidence: 99%

In vitro protein digestibility of animal, vegetal and microbial feed ingredients for Macrobrachium tenellum

Montoya-Martínez

Nolasco-Soria

Vega-Villasante

et al. 2018

LAJAR

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ABSTRACT. Due to the cost of raw materials, the need to formulate balanced feeds with highly digestible ingredients is indispensable for the aquaculture feed industry. For this reason, the protein in vitro digestibility, assessed by the pH-stat method, of ingredients with potential of using them on the balanced feed for Macrobrachium tenellum, were evaluated. The relative protein digestibility was assessed in twelve feed ingredients, including animal (pork meal, feather poultry meal, prime poultry meal, turkey meal, fish meal, shrimp meal), vegetal (coconut paste, chickpea meal, soybean meal, wheat gluten) and microbial (yeast and Spirulina meal); casein (Hammerstein grade) was used as the reference protein. The highest relative protein digestibility was found in: Spirulina meal (52.6%); following by pork meal (45.6%), and feather poultry meal (39.6%). The lowest digestibilities were found in soybean meal (15.9%), chickpea meal (12.1%), and fish meal (11.6%). The protein digestibility value should be considered for selecting potential ingredients for the formulation of balanced feeds for M. tenellum.

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“…AOAC International (1997;Swaisgood and Catignani, 1991). This method imitates the digestive function of livestock (Moyano et al, 2015). In this research, the determination of IvPD using pepsin method.…”

Section: Research Design and Data Analysismentioning

confidence: 99%

Characteristics of Feather Protein Concentrates Hydrolyzed Using Bacillus subtilis FNCC 0059

Said¹,

Abustam²,

Yuliati³

et al. 2018

OnLine Journal of Biological Sciences

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Fermentation technology is one technology that can be applied in the process of hydrolysis and decomposition of keratin protein in chicken feather waste. The fermentation technology can use microbial bacteria as a decomposer to improve the digestibility of the Feather Waste (FW). The purpose of this study was to evaluate the properties of chicken feather waste and Feather Protein Concentrate (FPC) fermented using Bacillus subtilis FNCC 0059. The properties of the feather-waste as control and FPC were studied specifically related to the dry matter protein digestibility properties performed in-vitro, water content, protein content, fat content, fiber content, NDF levels and amino acid profile. A total of 2 types of samples are compared the properties, namely FPC and FW. Bacterial isolates of Bacillus subtilis FNCC 0059 enriched with nutrient broth and propagated in a mixture of molasses and nutrient broth solution as a fermentation agent. The fermentation process was carried out for 4 weeks in the incubator at 35 °C. The data were processed statistically by using Ttest to compare the properties of FW with FPC fermentation for 4 weeks. The results showed that the properties of In-vitro Protein Digestibility (IvPD), moisture content, fiber content, NDF significantly differed (p>0.05) between FW and FPC, while protein and fat content were not significantly different (p>0.05). The amino acid profile in both samples has similarities.

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Application of in vitro digestibility methods in aquaculture: constraints and perspectives

Cited by 53 publications

References 134 publications

Black soldier fly (Hermetia illucens) pre-pupae meal as a fish meal replacement in diets for European seabass (Dicentrarchus labrax)

Black soldier fly (Hermetia illucens) pre-pupae meal as a fish meal replacement in diets for European seabass (Dicentrarchus labrax)

In vitro protein digestibility of animal, vegetal and microbial feed ingredients for Macrobrachium tenellum

Characteristics of Feather Protein Concentrates Hydrolyzed Using Bacillus subtilis FNCC 0059

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