Application of <i>RHD</i> and <i>RHCE</i> genotyping for correct blood group determination in chronically transfused patients

Legler, Tobias J.; Eber, Stefan; Lakomek, M.; Lynen, R.; Maas, Jens-Holger; Pekrun, Arnulf; Repas‐Humpe, M.; Schröter, W.; Köhler, Michael

doi:10.1046/j.1537-2995.1999.39080852.x

Cited by 56 publications

(47 citation statements)

References 28 publications

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“…The need for an easy and reliable method of identifying original blood group antigen status has led several groups to use DNA from blood as template. [8][9][10][11][12] These studies show a high degree of concordance between genotype and phenotype indicating that DNA typing is sufficient in most cases. There is, however, an advantage with our protocol that the RHC/c detection can be simultaneously carried out in one single PCR reaction without the need for RFLP and gel electrophoresis.…”

Section: Resultsmentioning

confidence: 74%

“…There is, however, an advantage with our protocol that the RHC/c detection can be simultaneously carried out in one single PCR reaction without the need for RFLP and gel electrophoresis. 8,10,13 The epitope difference between RHC and RHc is encoded in exon 2 and what identifies the RHC allele is identical to the RHD genotype. The difference between Rhc and RhC/D is enclosed in a 4247 basepair stretch of near nucleotide identity between the two genes making specific DNA detection of the RHC allele by simple PCR virtually impossible in RHD positive individuals.…”

Section: Resultsmentioning

confidence: 99%

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Accurate Rh phenotype determination by reticulocyte mRNA typing shortly after multiple transfusions

Randen¹,

Sørensen²,

Hauge³

et al. 2008

Haematologica

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Alloimmunization is a common phenomenon after transfusion, with an estimated incidence of 0.5% increasing to 20-60% in chronically transfused patients. In recently transfused patients, serological typing can be hampered by mixed field agglutination. We established RT-PCR methods for RHD, RHC/c and RHE/e typing using mRNA from reticulocytes. Molecular typing was performed soon after 51 separate mismatched transfusion events involving 30 patients. Accurate identification of the transfused patients' phenotype was confirmed in all cases. Reticulocyte maturation studies revealed that temperature is a crucial parameter for transition into mature red blood cells.

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Section: Resultsmentioning

confidence: 74%

Section: Resultsmentioning

confidence: 99%

Accurate Rh phenotype determination by reticulocyte mRNA typing shortly after multiple transfusions

Randen¹,

Sørensen²,

Hauge³

et al. 2008

Haematologica

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show abstract

“…Therefore, molecular techniques are increasingly applied to predict RhCE antigens [22]. However, because different ethnic groups have different genetic background and especially numerous variant RHCE alleles, many DNA genotyping techniques were found not reliable.…”

Section: Discussionmentioning

confidence: 99%

Rh-Matched Transfusion through Molecular Typing for β-Thalassemia Patients Is Required and Feasible in Chinese

Shao

Zhao

et al. 2018

Transfus Med Hemother

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Background: Molecular typing for RHCE blood group alleles has been established in many countries for patients and blood donors. In the Chinese literature nearly 80% of transfused patients with alloimmunization have antibodies specific for antigens of the Rh blood group system. We investigated if it is feasible to match packed red blood cells (RBCs) for Chinese β-thalassemia patients by RHCE genotyping. Methods: In this study, 481 patients with β-thalassemia were enrolled. They were genotyped for RHCE alleles by a simple PCR method with sequence-specific primers (PCR-SSP). Among these patients, 203 continuously received RBCs of the identical Rh subgroups according to the genotyping results for at least 3 months. Subsequently, their phenotypes were tested through a micro-column gel card method. For validation purposes, 400 donors were serologically typed with the same technology, of which 164 were genotyped too. Finally, the C, c, E, and e frequencies and the feasibility of the simple genotyping method were analyzed. Results: All patients showed mixed-field agglutination in the Rh subgroup gel cards before the same Rh subgroups in blood donors were selected for blood transfusion. The results, however, lacked mixed-field agglutination in all 203 cases after transfusion with RBC concentrates selected for the patient's C, c, E, and e antigens for at least 3 months. The genotyping results of 164 donors were all consistent with the serological results. Whole coding regions of RHCE were sequenced in 7 individuals with weak c, E, or e antigens. In only one sample we observed a 1059G>A nucleotide mutation coding for a truncated RhCE polypeptide (GenBank KT957625), in the other 6 samples no sequence variant was found. Both patients and donors were predominantly CcEe and CCee, with a prevalence of 55.3% and 24.9% for patients or 49.3% and 31.3% for donors, respectively. It revealed that about 80% of Chinese could receive Rh-matched RBCs easily. Conclusion: A simple RHCE genotyping technique is safe enough for Rh-matched transfusion of β-thalassemia patients in Chinese Han.

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“…Pilot programs incorporating blood group genotyping followed by large scale interventional studies will need to be conducted to address the ever increasing cost of alloimmunization in chronically transfused patients [11,12].…”

Section: Discussionmentioning

confidence: 99%

Lifetime risk and characterization of red blood cell alloimmunization in chronically transfused patients with sickle cell disease

Woldie

Swerdlow²,

M.H.³

et al. 2015

Int J Blood Transfus Immunohematol

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Application of RHD and RHCE genotyping for correct blood group determination in chronically transfused patients

Cited by 56 publications

References 28 publications

Accurate Rh phenotype determination by reticulocyte mRNA typing shortly after multiple transfusions

Accurate Rh phenotype determination by reticulocyte mRNA typing shortly after multiple transfusions

Rh-Matched Transfusion through Molecular Typing for β-Thalassemia Patients Is Required and Feasible in Chinese

Lifetime risk and characterization of red blood cell alloimmunization in chronically transfused patients with sickle cell disease

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