Application of iChemExplorer in pharmaceutical pH stress testing

Qiu, Fenghe; Du, Lily; Soman, Ashish; Jankovsky, Corinne; Li, Chan

doi:10.1016/j.jpba.2012.12.027

Cited by 2 publications

(1 citation statement)

References 6 publications

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“…The iChemExplorer has been used with success in preparative chemistry to monitor a reaction in real-time 8 and also in pharmaceutical pH stress testing for the elucidation of degradation products and pathways. 9 It occurred to us that this simple, inexpensive, yet innovative alternative to other tools may be valuable for studying enzyme-catalysed protein digestion and organic chemistry. We present our findings here.…”

Section: Introductionmentioning

confidence: 99%

Application of a new interface for rapid optimisation of bio-catalysed processes: proteolytic digestion and an enzyme-catalysed transesterification as examples

Stencel

Leadbeater

2014

New J. Chem.

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The results of an evaluation of the iChemExplorer for the study of bio-catalysed processes are reported.The iChemExplorer comprises of a specially-designed sample tray and a control unit, the former of which replaces a traditional tray in an HPLC autosampler assembly. It can be heated and reaction mixtures can be agitated. The system has been used to study the trypsin digestion of insulin chain B, cytochrome c and bovine serum albumin as well as the lipase-catalysed transesterification reaction between ethyl benzoate and 1-butanol. Results and discussionWe decided to focus on trypsin digestion of proteins and a lipase-catalysed transesterification reaction. The goal for trypsin digestion was to begin with a small protein and prove the Fig. 1 The iChemExplorer module and sample tray.

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Section: Introductionmentioning

confidence: 99%

Application of a new interface for rapid optimisation of bio-catalysed processes: proteolytic digestion and an enzyme-catalysed transesterification as examples

Stencel

Leadbeater

2014

New J. Chem.

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A Tool for Reaction Monitoring in Real Time, the Development of a “Walk-Up Automated Reaction Profiling” System

Alimuddin,

Bernier,

Braganza

et al. 2024

J. Org. Chem.

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Optimization of chemical reactions requires a thorough analysis of reaction products and intermediates over a given time course. Chemical reactions are often analyzed by liquid chromatography-mass spectrometry (LC-MS), but generating LC-MS samples and data analysis is time-consuming and produces a significant amount of waste. We sought to remove the sample preparation and data analysis steps by implementing an iChemExplorer/Agilent LC-MS instrument as our reactor and analysis tool, coupled with an automated report generator of reaction progress over time. Herein, we show that our easy-to-use walkup automated reaction profiling (WARP) system can sample chemical reactions multiple times to produce a data-rich report of reaction progress over time.

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Application of iChemExplorer in pharmaceutical pH stress testing

Cited by 2 publications

References 6 publications

Application of a new interface for rapid optimisation of bio-catalysed processes: proteolytic digestion and an enzyme-catalysed transesterification as examples

Application of a new interface for rapid optimisation of bio-catalysed processes: proteolytic digestion and an enzyme-catalysed transesterification as examples

A Tool for Reaction Monitoring in Real Time, the Development of a “Walk-Up Automated Reaction Profiling” System

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