Application of immobilized invertase to continuous hydrolysis of concentrated sucrose solutions

Abstract

Invertase immobilized onto corn grits was utilized in the hydrolysis of highly concentrated sucrose solutions producting liquid sugar solutions containing glucose and fructose. Comparisons of conversion efficiencies of this immobilized invertase in a continuous stirredtank reactor and a plug-flow reactor indicated that the plug-flow reactor has an higher efficiency. Continuous sucrose hydrolysis was then performed in 0.1- and 1-L tubular reactors. This tenforld scaling-up was achieved without any noticeable loss in efficiency. This process thus was scaled-up to a 17.6-L pilot reactor set in a cane sugar refinery. This reactor was fed with highly concentrated sucrose solutions [71% (w/w)] to produce invert sugar syrup with the desired inversion degree. It allows a productivity equal to 9.1 kg sucrose hydrolyzed/h in the case of a 69% (w/w) sucrose initial concentration with a 72% conversion rate.

Invertase immobilized on spacer-arm attached poly(hydroxyethyl methacrylate) membrane: Preparation and properties

“…The product (sugar mixture) obtained by invertase has the advantage of being colorless compared to the colored version obtained through acid hydrolysis. [12][13][14][15] Immobilization of invertase on natural polymers such as corn grits, 16 gelatin, 17 and various cellulose derivatives 18 has already been achieved, while its immobilization onto hydrogel polymers is limited. The latter has good chemical and mechanical stability, and is not susceptible to microbial attack.…”

Invertase immobilised on montmorillonite: reusability enhancement and reduction in leaching

“…Soluble invertase is used in the sweet industry in the production of artificial honey. Immobilisation of invertase on corn grits [3], polymer matrices [4][5][6][7], calcium alginate [8], composite gel fibres [9] etc., has already been reported. Studies on reusability of the immobilised enzymes are also reported.…”

Immobilization of the recombinant invertase INVB from Zymomonas mobilis on Nylon-6

“…These data suggest that the kinetic parameters of re-INVB were affected, when immobilized on Nylon-6, probably due to steric hindrance between the immobilized enzyme and the insoluble support [23]. A decline in catalytic activity for the invertase and other glycoenzymes upon immobilization has been reported [15,24,25]. Immobilization of invertase of yeast in poly(maleic anhydride-hexen-1) membrane [26] or in poly(vinyl alcohol) membrane suggested that the formation of the enzyme-substrate complex was more difficult for immobilized invertase, than for native invertase [27].…”