Application of immunohistochemistry for detection of metastases in sentinel lymph nodes of non-advanced breast cancer patients

Nowikiewicz, Tomasz; Śrutek, Ewa; Zegarski, Wojciech

doi:10.5114/pjp.2015.51149

Cited by 6 publications

(5 citation statements)

References 22 publications

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“…According to international experts' recommendations, in specific clinical situations, a withdrawal from the auxiliary axillary lymphadenoctomy is currently the standard procedure [16]. This is confirmed by our earlier studies, in which complete safety of implementation of the above changes was demonstrated [28][29][30]. However, a comprehensive discussion of them exceeds the framework of this study.…”

Section: Discussionsupporting

confidence: 72%

Analysis of sparing surgical procedures use in breast cancer patients at the Oncology Centre in Bydgoszcz in 2002–2017

Nowikiewicz¹,

Zegarski²

2018

Nowotwory. Journal of Oncology

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Section: Discussionsupporting

confidence: 72%

Analysis of sparing surgical procedures use in breast cancer patients at the Oncology Centre in Bydgoszcz in 2002–2017

Nowikiewicz¹,

Zegarski²

2018

Nowotwory. Journal of Oncology

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“…Diverse methods have been used to determine SLN status intraoperatively, e.g. frozen section histology (FS) [ 2 ], touch imprint cytology (TIC) [ 3 ], immunohistochemistry [ 4 ] and infrared spectroscopy [ 5 ]. TIC is considered a more tissue conserving and less expensive method compared to FS with comparable accuracy [ 6 ].…”

Section: Introductionmentioning

confidence: 99%

“…TIC is considered a more tissue conserving and less expensive method compared to FS with comparable accuracy [ 6 ]. The sensitivity of the TIC test on SLN in breast cancer is not consistent between studies, and seems to vary according to different tumor types, tumor size and the age of the patient [ 4 – 10 ]. Especially metastasis of invasive lobular carcinoma in the SLN is reported to be more difficult to identify on pathological examination compared to ductal carcinoma.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of intraoperative touch imprint cytology on axillary sentinel lymph nodes in invasive breast carcinomas, a retrospective study of 1227 patients comparing sensitivity in the different tumor subtypes

et al. 2018

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BackgroundIntraoperative evaluation of the axillary sentinel lymph node (SLN) in patients with breast carcinoma reduces the need of re-operations in cases where an axillary completion lymph node dissection (CLND) is indicated. Different methods have been used to determine the SLN status intraoperatively, e.g. frozen section histology (FS) and touch imprint cytology (TIC). The sensitivity of intraoperative TIC examination on SLN is not consistent between different studies and varies according to different tumor histologic subtypes, tumor size and the age of the patient. The aim of this study was to describe the specificity and sensitivity of TIC and to compare TIC sensitivity in the different histological subtypes of breast carcinoma.MethodsA retrospective review was performed of 1227 consecutive clinically node negative breast cancer patients treated with sentinel lymph node biopsy (SLNB) with intraoperative TIC between the years 2003 and 2008. The SLN was bisected and stained using the May-Grünwald-Giemsa method and immunocytochemically with the antibody MNF-116.ResultsThe overall sensitivity of the TIC test was 68.6% and the specificity was 99.8%. There was no statistically significant difference between the detection of SLN metastases from ductal carcinoma versus lobular carcinoma. The sensitivity improved over the period of the study.ConclusionTIC is highly specific with an acceptable overall sensitivity. The sensitivity increased under the period of the study and it was higher in cases with larger size of the primary tumor. There was no difference in TIC sensitivity between the different histological subtypes.

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“…Intraoperative analysis of frozen tissue sections using HE staining enables diagnosis within 20 min at many institutions, 28 although it can be difficult to diagnose with HE staining, especially for small tumor foci in lymph nodes 29,30 and surgical margins. 31 IHC analysis is sufficient to detect small numbers of tumor cells in lymph nodes, 29,30,32 although a standard IHC procedure commonly requires >30 min to complete, which makes its clinical application for intraoperative diagnosis impractical. Thus, the application of new methodology for intraoperative pathological evaluation is important.…”

Section: Discussionmentioning

confidence: 99%

Magnetically Promoted Rapid Immunofluorescence Staining for Frozen Tissue Sections

Onishi

Matsuda

Nakamura

et al. 2019

J Histochem Cytochem.

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Current immunohistochemistry methods for diagnosing abnormal cells, such as cancer cells, require multiple steps and can be relatively slow compared with intraoperative frozen hematoxylin and eosin staining, and are therefore rarely used for intraoperative examination. Thus, there is a need for novel rapid detection methods. We previously demonstrated that functionalized fluorescent ferrite beads (FF beads) magnetically promoted rapid immunoreactions. The aim of this study was to improve the magnetically promoted rapid immunoreaction method using antibody-coated FF beads and a magnet subjected to a magnetic field. Using frozen sections of xenograft samples of A431 human epidermoid cancer cells that express high levels of epidermal growth factor receptor (EGFR) and anti-EGFR antibody-coated FF beads, we reduced the magnetically promoted immunohistochemistry procedure to a 1-min reaction and 1-min wash. We also determined the optimum magnetic force for the antibody reaction (from 7.79 × 10−15 N to 3.35 × 10−15 N) and washing (4.78 × 10−16 N), which are important steps in this technique. Furthermore, we stained paraffin-embedded tissue arrays and frozen sections of metastatic breast cancer lymph nodes with anti-pan-cytokeratin antibody-coated FF beads to validate the utility of this system in clinical specimens. Under optimal conditions, this ultra-rapid immunostaining method may provide an ancillary method for pathological diagnosis during surgery. (J Histochem Cytochem 58:XXX–XXX, 2010)

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Application of immunohistochemistry for detection of metastases in sentinel lymph nodes of non-advanced breast cancer patients

Cited by 6 publications

References 22 publications

Analysis of sparing surgical procedures use in breast cancer patients at the Oncology Centre in Bydgoszcz in 2002–2017

Analysis of sparing surgical procedures use in breast cancer patients at the Oncology Centre in Bydgoszcz in 2002–2017

Evaluation of intraoperative touch imprint cytology on axillary sentinel lymph nodes in invasive breast carcinomas, a retrospective study of 1227 patients comparing sensitivity in the different tumor subtypes

Magnetically Promoted Rapid Immunofluorescence Staining for Frozen Tissue Sections

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