Application of immunoproteomics in developing a Streptococcus iniae vaccine for olive flounder (Paralichthys olivaceus)

Immunoprotection of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) from Lactococcus garvieae against Lactococcosis in tilapia

“…In analyzing the immunogenicity of this protein, Western blotting of rabbit serum and L. garvieae-infected tilapia sera against GAPDH, respectively, revealed that both sera reacted strongly with purified recombinant GAPDH proteins. A similar observation was made when carried out using rabbit and olive flounder sera based on 2-DE (Shin et al, 2007(Shin et al, , 2009.…”

“…Proteomic analysis based on 2-DE revealed that the protein profiles of the L. garvieae strains differ from each other. Shin et al noted a similar phenomenon when using rabbit sera (Shin et al, 2007). Those studies also observed GAPDH and other common antigens found in L. garvieae stains (Shin et al, 2007(Shin et al, , 2009.…”

“…Shin et al noted a similar phenomenon when using rabbit sera (Shin et al, 2007). Those studies also observed GAPDH and other common antigens found in L. garvieae stains (Shin et al, 2007(Shin et al, , 2009. Moreover, despite studies citing GAPDH as a promising vaccine to protect against Edwardsiella tarda, Vibrio anguillarum and S. pneumonia (Ling et al, 2004;Liu et al, 2005Liu et al, , 2007, efficacy of the GAPDH vaccine from L. garvieae has not been investigated.…”

“…Protective in several ways, GAPDH may protect antigenicity against other pathogenic bacteria in fish . Shin et al (2007Shin et al ( , 2009 found that L. garvieae GAPDH, with its high antigenicity for inducing an immunity response, is highly promising for use in developing an effective vaccine against L. garvieae in fish (Shin et al, 2006(Shin et al, , 2007(Shin et al, , 2009). Our previous study noted a similar finding, indicating that GAPDH can protect fish against L. garvieae.…”

Comparison of the Efficacy of Selected Bacterins against Edwardsiella Tarda in Immunized Japanese eel (Anguilla japonica)

“…The problems of vaccination with formalin-killed vaccine against bacterial infection have been reviewed by a number of authors 37 . Until recently, they related to a limited understanding of the immune response -in particular the role of the systemic and mucosal adaptive immune responses -and the relationship of both the humoral and cell-mediated systems to the vaccination with low concentration of formalin with heat, citric acid, pressure and electric current-killed vaccine against E. tarda infection.…”