Application of Induced Pluripotent Stem Cell Technology to the Study of Hematological Diseases

Li, Mailin; Cascino, Pasquale; Ummarino, Simone; Ruscio, Annalisa Di

doi:10.3390/cells6010007

Cited by 14 publications

(12 citation statements)

References 64 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The ratio of chl a/chl b was decreased, while an increase was observed in total chl/carotenoids ratio (Cheng & Cheng, 2012). The increase in chl a/chl b was reported as an indicator of chlorophyll b degradation and its conversion to chl a under stressed conditions (Folly & Engel, 1999; Gomes et al, 2017; Longo et al, 2017).…”

Section: Discussionmentioning

confidence: 99%

Modulation of biochemical and physiological parameters in Hordeum vulgare L. seedlings under the influence of benzyl-butyl phthalate

Kumari

Kaur

2019

PeerJ

View full text Add to dashboard Cite

Background Phthalates are man-made chemical compounds with numerous applications especially known for their use as plasticizers. They have weak bonding to the polymeric matrix or products in which they are used. Owing to this reason, they are readily released into the environment which makes them ubiquitous. The agricultural soils are also reported to be polluted with phthalates up to a considerable extent which causes adverse effects on flora and fauna. A few studies have been conducted on phthalate-induced phytotoxicity, which has revealed that phthalates affect the quality and yield of edible plants. In the last decades, some crops were analyzed for phthalate-induced adversities; among them, barley was the least explored. Methods The present study has investigated the impact of benzyl-butyl phthalate (BBP) on barley (Hordeum vulgare L.) seedlings to address the biochemical, physiological consequences, and toxicological implications. After the exogenous exposure of BBP (viz. 0, 25, 50, 100, 200, 400, 800, 1,600 mg/L) for 7 days, barley seedlings were analyzed for different indices. Results The exposure of BBP mediated a significant (p ≤ 0.05, 0.01) overall elevation in the contents of pigment, proline, soluble protein, carbohydrate, hydrogen peroxide (H2O2), and malondialdehyde (MDA) in shoots and roots of barley seedlings. The activities of superoxide dismutase (SOD), guaiacol peroxidase (POD), catalase (CAT), ascorbate peroxidase (APX), and glutathione reductase (GR) were also stimulated significantly in shoots and roots of seedlings against BBP stress except for SOD activity which declined in the roots. The polyphenols (non-enzymatic antioxidants) content was also altered in all the treated concentrations as compared to the control. Furthermore, BBP caused stomatal abnormalities, induced cytotoxicity, and loss of plasma membrane integrity. Conclusions BBP disturbed the normal physiology of barley which could also affect the yield of the crop under field conditions.

show abstract

Section: Discussionmentioning

confidence: 99%

Modulation of biochemical and physiological parameters in Hordeum vulgare L. seedlings under the influence of benzyl-butyl phthalate

Kumari

Kaur

2019

PeerJ

View full text Add to dashboard Cite

show abstract

“…Stem cells are undifferentiated cells defined by their properties of self-renewal and potency and are rare in nature [ 45 ]. Breast cancers contain CSCs, and these cells are thought to be involved in tumor initiation, progression, evolution, and metastasis [ 46 ].…”

Section: Biology and Biomarkers Of Breast Cscsmentioning

confidence: 99%

Heterogeneity of Tumors in Breast Cancer: Implications and Prospects for Prognosis and Therapeutics

Tuasha

Petros

2020

Scientifica

View full text Add to dashboard Cite

Breast cancer is the most commonly diagnosed form of cancer in women comprising 16% of all female cancers. The disease shows high intertumoral and intratumoral heterogeneity posing diagnostic and therapeutic challenges with unpredictable clinical outcome and response to existing therapy. Mounting evidence is ascertaining that breast cancer stem cells (CSCs) are responsible for tumor initiation, progression, recurrence, evolution, metastasis, and drug resistance. Therapeutics selectively targeting the CSCs based on distinct surface molecular markers and enhanced intracellular activities of these cells continue to evolve and hold significant promise. Having plethora of heterogeneity accompanied with failure of existing conventional therapeutics and poor prognosis, the present review focuses on elucidating the main signaling pathways in breast CSCs as major therapeutic targets. The role of developments in nanomedicine and miRNA as targeted delivery of therapeutic anticancer agents is also highlighted.

show abstract

“…Due to transformation concerns, many groups later replaced the c-Myc proto-oncogene [ 12 , 13 ] with less dangerous and programmable genes, such as PARP1 [ 14 , 15 , 16 , 17 , 18 , 19 ]. Since the landmark finding that lineage-restricted cells can be converted to a pluripotent state, several iPS cell lines have been obtained from patients affected by congenital and acquired hematological diseases, including leukemia, with the purpose to establish disease modeling and identify novel therapeutic targets [ 20 , 21 , 22 ].…”

Section: Ips Cells Technologymentioning

confidence: 99%

Induced Pluripotent Stem Cell-Derived Red Blood Cells and Platelet Concentrates: From Bench to Bedside

Focosi¹,

Amabile

2017

Cells

View full text Add to dashboard Cite

Red blood cells and platelets are anucleate blood components indispensable for oxygen delivery and hemostasis, respectively. Derivation of these blood elements from induced pluripotent stem (iPS) cells has the potential to develop blood donor-independent and genetic manipulation-prone products to complement or replace current transfusion banking, also minimizing the risk of alloimmunization. While the production of erythrocytes from iPS cells has challenges to overcome, such as differentiation into adult-type phenotype that functions properly after transfusion, platelet products are qualitatively and quantitatively approaching a clinically-applicable level owing to advances in expandable megakaryocyte (MK) lines, platelet-producing bioreactors, and novel reagents. Guidelines that assure the quality of iPS cells-derived blood products for clinical application represent a novel challenge for regulatory agencies. Considering the minimal risk of tumorigenicity and the expected significant demand of such products, ex vivo production of iPS-derived blood components can pave the way for iPS translation into the clinic.

show abstract

Application of Induced Pluripotent Stem Cell Technology to the Study of Hematological Diseases

Cited by 14 publications

References 64 publications

Modulation of biochemical and physiological parameters in Hordeum vulgare L. seedlings under the influence of benzyl-butyl phthalate

Modulation of biochemical and physiological parameters in Hordeum vulgare L. seedlings under the influence of benzyl-butyl phthalate

Heterogeneity of Tumors in Breast Cancer: Implications and Prospects for Prognosis and Therapeutics

Induced Pluripotent Stem Cell-Derived Red Blood Cells and Platelet Concentrates: From Bench to Bedside

Contact Info

Product

Resources

About