Application of inkjet printing technique for biological material delivery and antimicrobial assays

Zheng, Qiang; Lu, Jiangang; Chen, Hao; Huang, Lei; Cai, Jin; Xu, Zhinan

doi:10.1016/j.ab.2010.10.024

Cited by 58 publications

(42 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…For instance, DOD mode micro-droplet deposition has been employed for high-throughput screening (HTS) applications to increase the efficiency of drug screening and delivery [20][21][22].…”

Section: Introductionmentioning

confidence: 99%

Alternating Force Based Drop-on-Demand Microdroplet Formation and Three-Dimensional Deposition

Zhao

Yan

Yao

et al. 2015

Journal of Manufacturing Science and Engineering

View full text Add to dashboard Cite

AbstractsDrop-on-demand (DOD) micro-droplet formation and deposition play an important role in additive manufacturing, particularly in printing of 3D in vitro biological models for pharmacological and pathological studies, for tissue engineering and regenerative medicine applications, and for building of cell-integrated micro-fluidic devices. In development of a DOD based micro-droplet deposition process for 3D cell printing, the droplet formation, controlled on-demand deposition and at the single cell level, and most importantly, maintaining the viability and functionality of the cells during and after the printing, are all remaining to be challenged. This report presents our recent study on developing a novel DOD based micro-droplet deposition process for 3D Printing by utilization of an alternating viscous & inertial force jetting mechanism. The results include an analysis of droplet formation mechanism, the system configuration, and experimental study of the effects of process parameters on micro-droplet formation. Sodium Alginate solutions are used for micro-droplet formation and deposition. Key process parameters include actuation signal waveforms, nozzle dimensional features, and solution SUN A c c e p t e d M a n u s c r i p t N o t C o p y e d i t e dviscosity. Sizes of formed micro-droplets are examined by measuring the droplet diameter and velocity. Resultsshow that by utilizing a nozzle at a 45μm diameter, the size of the formed micro-droplets are in the range of 52~72μm in diameter and 0.4~2.0m/s in jetting speed, respectively. Reproducibility of the system is also examined and the results show that the deviation of the formed micro-droplet diameter and the droplet deposition accuracy are within 6% and 6.2μm range, respectively. Experimental results demonstrate a high controllability and precision for the developed DOD micro-droplet deposition system with a potential for precise cell printing.

show abstract

“…For instance, DOD mode micro-droplet deposition has been employed for high-throughput screening (HTS) applications to increase the efficiency of drug screening and delivery [20][21][22].…”

Section: Introductionmentioning

confidence: 99%

Alternating Force Based Drop-on-Demand Microdroplet Formation and Three-Dimensional Deposition

Zhao

Yan

Yao

et al. 2015

Journal of Manufacturing Science and Engineering

View full text Add to dashboard Cite

show abstract

“…The methodology requires a labeled biomolecule, a sophisticated camera, and a label-free detection device such as a quartz crystal microbalance (QCM). Recently, several researchers have used specifically labeled materials to construct three dimensional patterns in a layer-by-layer manner to generate artificial cells that mimic body tissue (Zheng et al 2011;Matsusaki et al 2011;Kettle, Lamminmäki, and Gane 2010). Although fluorescent labeling reagents are useful for approximating the amount of liquid deposited, light interference may prevent accurate determination.…”

Section: Introductionmentioning

confidence: 99%

“…Inkjet patterning has been developed to deposit micro amounts of biomedical fluids and to print three-dimensional structures (Zheng et al 2011;Xu et al 2005;Umezu et al 2011;Setti et al 2005;Kim et al 2010;Desai et al 2010;Abe, Suzuki, and Citterio 2008;Yatsushiro et al 2011). Picoliter quantities may be deposited and patterned in a single process with drop-on-demand control.…”

Section: Introductionmentioning

confidence: 99%

Inexpensive and Reliable Monitoring of the Microdeposition of Biomolecules

Fuchiwaki¹,

Tanaka²,

Ooie³

2015

Analytical Letters

View full text Add to dashboard Cite

Anti-C-reactive protein antibody is used to quantify C-reactive protein in diagnostic tests for assessing inflammation levels. In order to develop a practical chip for diagnostic tests, anti-C-reactive protein antibody was continuously ejected using a piezoelectric injector and microdeposition was monitored on a shot-by-shot basis using a 30 MHz quartz crystal microbalance. Background noise due to external sources such as air flow and temperature fluctuations were largely eliminated by covering the quartz crystal microbalance with a stainless-steel cover and maintaining a fixed distance between the optical microscope lamp and the piezoelectric injector. Each shot of a 1 mg ml À1 solution of anti-C-reactive protein antibody solution delivered approximately 30 pl to the quartz crystal microbalance. The frequency change per shot was 2.21 AE 0.97 Hz and the injected mass was estimated to be 44.2 pg using the Sauerbrey equation. This approach simplifies the experimental evaluation of inkjet bioprinting by allowing the sensitive determination of shot ejection mass and volume, and realizes label-free detection, continuous monitoring, and simple temperature control. Measurements can be performed at atmospheric pressure and at room temperature. Furthermore, the system uses only commercially available devices.

show abstract

“…Thermal inkjet printers use a heating element to vaporize a small volume of fluid, generating a bubble that expands and ejects a controlled volume of fluid as a single droplet. The main concern associated to thermal inkjet relies on the high temperatures generated close to the heating element during the printing process, which may affect the biomolecules and cells to be printed [170,171,213]. However, it has been demonstrated that, due to the short duration of the heating pulses (*2 ls), the increase in temperature of the bioink is only a few degrees, allowing the printing of viable cells with a low percentage of cell dead [35].…”

Section: Inkjet Bioprintingmentioning

confidence: 99%

Advances in bioprinted cell-laden hydrogels for skin tissue engineering

et al. 2017

View full text Add to dashboard Cite

Bioprinting technologies are powerful additive biofabrication techniques to produce cellular constructs for skin tissue engineering owing to their unique ability to precisely pattern living and non-living materials in predefined spatial locations. This unique feature, combined with the computer controlled printing and medical imaging techniques, enable researchers and clinicians to generate patient specific constructs partly replicating the intricate compositional and architectural organization of the skin. Bioprinting has been used to automatically dispense hydrogels with skin cells located in prescribed sites that promote skin formation in vitro and in vivo. Current skin bioprinting approaches mostly rely on the sequential printing of fibroblasts and keratinocytes embedded within a homogeneous hydrogel. Although such approaches have already been translated to pre-clinical scenarios, they still present limitations in terms of fully replicating the cellular and extracellular matrix (ECM) heterogeneity in native skin. The success of bioprinting for skin repair strongly depends on the design of printable bioinks capable of supporting the function of printed cells and stimulating the production of new ECM components. To better mimic the human skin, novel developments in dedicated bioprinting technologies, in the design of bioinks, as well as in the printing of vascularised constructs are necessary. This paper presents an overview regarding the use of bioprinting for skin tissue engineering applications. The operating principles of bioprinting technologies are outlined along with requirements of printed skin constructs. Finally, preclinical results are summarized and future perspectives for the field are highlighted.

show abstract

Application of inkjet printing technique for biological material delivery and antimicrobial assays

Cited by 58 publications

References 13 publications

Alternating Force Based Drop-on-Demand Microdroplet Formation and Three-Dimensional Deposition

Alternating Force Based Drop-on-Demand Microdroplet Formation and Three-Dimensional Deposition

Inexpensive and Reliable Monitoring of the Microdeposition of Biomolecules

Advances in bioprinted cell-laden hydrogels for skin tissue engineering

Contact Info

Product

Resources

About