Application of Interspersed Repetitive Sequence Polymerase Chain Reaction for Construction of Yeast Artificial Chromosome Contigs

Hunter, Kent W.

doi:10.1006/meth.1997.0541

Cited by 2 publications

(4 citation statements)

References 31 publications

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“…IRS is a widely distributed and highly conserved interval repeat sequence in gDNA. 47 The primer synthesized IRS‐PCR amplifies DNA between two IRS sequences. Alu sequence is a human unique IRS sequence, which contains about 900,000 copies in the human haploid genome, with an average interval of 4 kb.…”

Section: Pcr Wga Technologymentioning

confidence: 99%

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Recent advances and application of whole genome amplification in molecular diagnosis and medicine

Wang

Liu

et al. 2022

MedComm

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Whole genome amplification (WGA) is a technology for non‐selective amplification of the whole genome sequence, first appearing in 1992. Its primary purpose is to amplify and reflect the whole genome of trace tissues and single cells without sequence bias and to provide sufficient DNA template for subsequent multigene and multilocus analysis, along with comprehensive genome research. WGA provides a method to obtain a large amount of genetic information from a small amount of DNA and provides a valuable tool for preserving limited samples in molecular biology. WGA technology is especially suitable for forensic identification and genetic disease research, along with new technologies such as next‐generation sequencing (NGS). In addition, WGA is also widely used in single‐cell sequencing. Due to the small amount of DNA in a single cell, it is often unable to meet the amount of samples needed for sequencing, so WGA is generally used to achieve the amplification of trace samples. This paper reviews WGA methods based on different principles, summarizes both amplification principle and amplification quality, and discusses the application prospects and challenges of WGA technology in molecular diagnosis and medicine.

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Section: Pcr Wga Technologymentioning

confidence: 99%

“…IRS is a widely distributed and highly conserved interval repeat sequence in gDNA 47 . The primer synthesized IRS‐PCR amplifies DNA between two IRS sequences.…”

Section: Pcr Wga Technologymentioning

confidence: 99%

Recent advances and application of whole genome amplification in molecular diagnosis and medicine

Wang

Liu

et al. 2022

MedComm

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show abstract

“…2A, lanes [14][15][16][17], but was polymorphic among three golden retrievers and nine Doberman pinschers ( Fig. 2A, compare lanes 2-4 and lanes [5][6][7][8][9][10][11][12][13]. Marker COLIP was homozygous in all Doberman pinscher samples tested (Fig.…”

Section: Assessment Of Can-sine Informativenessmentioning

confidence: 99%

“…IRS-PCR using the Alu repeats has found wide application in human genetics. For example, in isolation of chromosome region-specific polymorphic markers (2), identification of chromosome-specific YACs (3), linkage mapping (40), and contig assembly (18,11). Utilizing B1/B2 repeats (9), IRS-PCR technology has supported the construction of integrated physical and genetic maps of the murine genome (10).…”

mentioning

confidence: 99%

A set of canine interrepeat sequence PCR markers for high-throughput genotyping

Das¹,

Sakul²,

Kong³

et al. 2000

Physiological Genomics

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One hundred and sixteen interspersed repetitive DNA sequence (IRS)-PCR markers have been developed and characterized from Canis familiaris for high-throughput filter-based genotyping. We present a detailed analysis of markers produced by amplification using primers directed to the conserved regions of the C. familiaris short interspersed nuclear element (Can-SINE). The majority of IRS-PCR markers developed were moderately to highly polymorphic with mean heterozygosity (HET) and polymorphism information content (PIC) values of approximately 0.6. The HET value for 22.3% of the markers exceeded 0.7. We also demonstrate that sequence variation of Can-SINEs between breeds is significant and also represents a rich source of polymorphisms. Mapping of 73 of the markers to the existing integrated linkage-radiation hybrid map enriches the map as well as establishes the utility of the markers. The significance and utility of this new class of IRS-PCR Can-SINE-based markers for high-throughput genotyping is discussed. This method can also be extended to other species that are currently map-poor but have a sufficiently high density of SINEs to allow IRS-PCR.

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Application of Interspersed Repetitive Sequence Polymerase Chain Reaction for Construction of Yeast Artificial Chromosome Contigs

Cited by 2 publications

References 31 publications

Recent advances and application of whole genome amplification in molecular diagnosis and medicine

Recent advances and application of whole genome amplification in molecular diagnosis and medicine

A set of canine interrepeat sequence PCR markers for high-throughput genotyping

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