Application of intracytoplasmic sperm injection (ICSI) for fertilization and development in birds

Shimada, Kiyoshi; Ono, Tamao; Mizushima, Shusei

doi:10.1016/j.ygcen.2013.11.001

Cited by 8 publications

(4 citation statements)

References 25 publications

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“…Indeed, the volume of quail egg cytoplasm is more than 1-2 µL, whereas that of the mouse is approximately 200 pL. 9 Egg-activating proteins equivalent to 100-200 sperm are required for fertilization and full-term development to hatching in quails, which supports the strong correlation between egg size and polyspermy (Birkhead et al 1994;Mizushima 2012Shimada et al 2014;Kang et al 2015).…”

Section: Egg Size and Sperm Numbermentioning

confidence: 90%

“…This finding suggests that the fertilization rate increases when the number of sperm incorporated into an egg is higher. In addition, the direct injection of sperm into the cytoplasm of a mature quail egg, namely, an intracytoplasmic sperm injection (ICSI), directly revealed that a single sperm was insufficient for a high rate of fertilization and subsequent blastoderm development (approximately 20%; Hrabia et al 2003;Takagi et al 2007a;Mizushima 2008Mizushima , 2009Mizushima , 2012Shimada et al 2014;Kang et al 2015). It has been proposed that the purpose of polyspermy is to increase the opportunity of a sperm nucleus migrating to the center of the germinal disc and making contact with the female nucleus because the surface area of the avian germinal disc in relation to that of sperm is very large.…”

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confidence: 99%

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Fertilization 2: Polyspermic Fertilization

Mizushima¹

2017

Advances in Experimental Medicine and Biology

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During fertilization in animals, a haploid egg nucleus fuses with a haploid sperm nucleus to restore the diploid genome. In most animals including mammals, echinoderms, and teleostei, the penetration of only one sperm into an egg is ensured at fertilization because the entry of two or more sperm is prevented by polyspermy block systems in these eggs. On the other hand, several animals such as birds, reptiles, and most urodele amphibians exhibit physiological polyspermy, in which the entry of several sperm into one egg is permitted. However, in these polyspermic eggs, only one sperm nucleus is involved in zygotic formation with a female nucleus, thereby avoiding syngamy with multiple sperm nuclei. In the chicken, 20-60 sperm are generally found within the egg cytoplasm at fertilization and this number is markedly higher than that of other polyspermic species; however, avian-specific events such as the degeneration and mitosis of supernumerary sperm nuclei during early embryo development allow a polyspermic egg to develop normally. This chapter describes current knowledge on polyspermy-related events in avian eggs during fertilization, and is characterized by a comparison to the fertilization modes of other vertebrates. The close relationship between sperm numbers and egg sizes, and the movement of supernumerary sperm nuclei towards the periphery of the egg cytoplasm and their degeneration are summarized. The molecular mechanisms by which polyspermy initiates egg activation to start embryo development are also discussed.

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Section: Egg Size and Sperm Numbermentioning

confidence: 90%

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confidence: 99%

Fertilization 2: Polyspermic Fertilization

Mizushima¹

2017

Advances in Experimental Medicine and Biology

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“…In the chicken, CRISPR/Cas9 has been used to knock out genes in somatic cells of the developing embryo [ 16 ], but no germline modifications have been reported. In birds, direct injection of constructs into the zygote and SCNT approaches are impractical, owing to the structure of the zygote, the opacity of the oocyte, and the difficulty in retrieving, manipulating, and culturing early embryos to term [ 17 , 18 ]. Direct in vivo transfection of germline cells in embryos by lipofection is an alternative strategy to introduce constructs but the efficiency of transfection is low [ 19 , 20 ].…”

Section: Introductionmentioning

confidence: 99%

Germline Gene Editing in Chickens by Efficient CRISPR-Mediated Homologous Recombination in Primordial Germ Cells

Dimitrov¹,

Pedersen²,

Ching³

et al. 2016

PLoS ONE

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The CRISPR/Cas9 system has been applied in a large number of animal and plant species for genome editing. In chickens, CRISPR has been used to knockout genes in somatic tissues, but no CRISPR-mediated germline modification has yet been reported. Here we use CRISPR to target the chicken immunoglobulin heavy chain locus in primordial germ cells (PGCs) to produce transgenic progeny. Guide RNAs were co-transfected with a donor vector for homology-directed repair of the double-strand break, and clonal populations were selected. All of the resulting drug-resistant clones contained the correct targeting event. The targeted cells gave rise to healthy progeny containing the CRISPR-targeted locus. The results show that gene-edited chickens can be obtained by modifying PGCs in vitro with the CRISPR/Cas9 system, opening up many potential applications for efficient genetic modification in birds.

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“…Thus, the relatively simple straightforward one-cell embryo microinjection procedures of generating genome edited mammalian embryos or SCNT cannot be easily replicated in birds as the early stage embryo is not accessible. Recent advances, however, have been made manipulating the early stage of ovum using intracytoplasmic sperm injection (ICSI) for fertilisation 24 and a surrogate shell for incubation. 25 However, this ICSI method has yet to be used for GE as few hatchlings were obtained from the ICSI embryos.…”

Section: Ge Of Bird Speciesmentioning

confidence: 99%

Genome editing of avian species: implications for animal use and welfare

Panda

McGrew

2021

Lab Anim

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Avian species are used as model systems in research and have contributed to ground-breaking concepts in developmental biology, immunology, genetics, virology, cancer and cell biology. The chicken in particular is an important research model and an agricultural animal as a major contributor to animal protein resources for the global population. The development of genome editing methods, including CRISPR/Cas9, to mediate germline engineering of the avian genome will have important applications in biomedical, agricultural and biotechnological activities. Notably, these precise genome editing tools have the potential to enhance avian health and productivity by identifying and validating beneficial genetic variants in bird populations. Here, we present a concise description of the existing methods and current applications of the genome editing tools in bird species, focused on chickens, with attention on animal use and welfare issues for each of the techniques presented.

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Application of intracytoplasmic sperm injection (ICSI) for fertilization and development in birds

Cited by 8 publications

References 25 publications

Fertilization 2: Polyspermic Fertilization

Fertilization 2: Polyspermic Fertilization

Germline Gene Editing in Chickens by Efficient CRISPR-Mediated Homologous Recombination in Primordial Germ Cells

Genome editing of avian species: implications for animal use and welfare

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