Application of Ion Chromatography for the Determination of Inorganic Ions, Especially Thiocyanates, in Human Semen Samples as Biomarkers of Environmental Tobacco Smoke Exposure

Demkowska, Ilona; Polkowska, Żaneta; Kiełbratowska, Bogumiła; Namieśnik, Jacek

doi:10.1093/jat/34.9.533

Cited by 11 publications

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“…The electroactive compounds commonly found in human saliva samples (ascorbic acid and uric acid) [50,51] were evaluated as possible interferents. As can be observed in Figure 7, both ascorbic acid (+0.90 V) and uric acid (+1.17 V) are oxidized at more negative potentials than TIB (>+1.20 V).…”

Section: Resultsmentioning

confidence: 99%

Electrochemical Determination of the Steroid Tibolone and Its Metabolites in Saliva Samples

et al. 2020

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Tibolone (TIB) is a synthetic steroid banned by the World Anti‐Doping Agency (WADA) due to its similar properties to estrogen and progesterone. In this work, a simple and low‐cost electrochemical method for the determination of TIB and its metabolites (3‐α hydroxytibolone and 3‐β hydroxytibolone) in authentic human saliva is reported. Square‐wave voltammetry (SWV) with an unmodified boron‐doped diamond (BDD) electrode and phosphate buffer 0.1 mol L−1 were selected as the electrochemical technique, working electrode, and supporting electrolyte, respectively. Analytical characteristics of the proposed method include good repeatability (RSD <2.4 %), wide linear range (1 to 110 μmol L−1; r=0.999) and low detection limit (0.3 μmol L−1). Average recovery values (104±3 % for TIB and 98±5 % for the metabolites) for direct analysis of spiked saliva samples (only diluted in supporting electrolyte) indicate that no significant matrix interference was observed. The proposed method was also efficient for the determination of TIB in pharmaceutical samples (statistically similar results to those obtained by HPLC).

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Section: Resultsmentioning

confidence: 99%

Electrochemical Determination of the Steroid Tibolone and Its Metabolites in Saliva Samples

et al. 2020

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“…Regarding the publications, it is possible to use other biological samples such as liver [15], semen [78], or nasal fluid [79] to determine these biomarkers.…”

Section: Biological Specimens and Cut-off Concentrationsmentioning

confidence: 99%

Recent Developments in the Determination of Biomarkers of Tobacco Smoke Exposure in Biological Specimens: A Review

Marques

Cruz-Vicente

Rosado

et al. 2021

IJERPH

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Environmental tobacco smoke exposure (ETS) and smoking have been described as the most prevalent factors in the development of certain diseases worldwide. According to the World Health Organization, more than 8 million people die every year due to exposure to tobacco, around 7 million due to direct ETS and the remaining due to exposure to second-hand smoke. Both active and second-hand exposure can be measured and controlled using specific biomarkers of tobacco and its derivatives, allowing the development of more efficient public health policies. Exposure to these compounds can be measured using different methods (involving for instance liquid- or gas-chromatographic procedures) in a wide range of biological specimens to estimate the type and degree of tobacco exposure. In recent years, a lot of research has been carried out using different extraction methods and different analytical equipment; this way, liquid–liquid extraction, solid-phase extraction or even miniaturized procedures have been used, followed by chromatographic analysis coupled mainly to mass spectrometric detection. Through this type of methodologies, second-hand smokers can be distinguished from active smokers, and this is also valid for e-cigarettes and vapers, among others, using their specific biomarkers. This review will focus on recent developments in the determination of tobacco smoke biomarkers, including nicotine and other tobacco alkaloids, specific nitrosamines, polycyclic aromatic hydrocarbons, etc. The methods for their detection will be discussed in detail, as well as the potential use of threshold values to distinguish between types of exposure.

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“…The previously conducted studies indicate that the thiocyanate ion can be determined in biological fluids using ion chromatography. It was determined in samples of saliva (concentration range: heavy smokers 0.015-0.847 mg/g, 0.046-0.7 mg/g moderate smokers, passive smokers 0.046-0.277 mg/g) and in samples of semen (concentration range: heavy smokers 0.00004-0.058 mg/g, moderate smokers 0.00008-0.043 mg/g, passive smokers 0.00004-0.028 mg/g) [22,23]. Moreover, the results on thiocyanate ion determination in the samples of saliva collected from the mothers, Pearson's correlation coefficient values for the relationships between ion concentrations in the samples of meconium and maternal saliva and F-Snedecor and t-students test to ion concentrations in meconium samples are reported.…”

Section: Sample Collection and Preparationmentioning

confidence: 99%

Meconium samples used to assess infant exposure to the components of ETS during pregnancy

Narkowicz

Polkowska

Kiełbratowska

et al. 2015

Int J Occup Med Environ Health

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International Journal of Occupational Medicine and EnvironmentalThe aim of the study was to use meconium samples to assess fetal exposure to compounds present in environmental tobacco smoke (ETS). Material and Methods: In order to assess fetal exposure to toxic tobacco smoke compounds, samples of meconium from the offspring of women with different levels of tobacco smoke exposure, and the samples of saliva from the mothers were analyzed. Thiocyanate ion as a biomarker of tobacco smoke exposure, and other ions that are indices of such exposure were determined by means of ion chromatography. Results: The results of ion chromatography analysis of the meconium and maternal saliva samples for the presence of cations and anions (including thiocyanate ion) indicate that the concentration level of specific ions depends on the intensity of environmental tobacco smoke exposure of pregnant women. Conclusions: Based on the results, it can be concluded that meconium samples can be used to determine the substances from tobacco smoke. The results confirm the effect of smoking during pregnancy on the presence and content of substances from tobacco smoke.

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Application of Ion Chromatography for the Determination of Inorganic Ions, Especially Thiocyanates, in Human Semen Samples as Biomarkers of Environmental Tobacco Smoke Exposure

Cited by 11 publications

References 0 publications

Electrochemical Determination of the Steroid Tibolone and Its Metabolites in Saliva Samples

Electrochemical Determination of the Steroid Tibolone and Its Metabolites in Saliva Samples

Recent Developments in the Determination of Biomarkers of Tobacco Smoke Exposure in Biological Specimens: A Review

Meconium samples used to assess infant exposure to the components of ETS during pregnancy

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