Application of isotachophoresis in food analysis

Kvasnička, František

doi:10.1002/1522-2683(20000801)21:14<2780::aid-elps2780>3.0.co;2-w

Cited by 35 publications

(15 citation statements)

References 49 publications

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“…A Fibertec Fresh plant and silage pH were determined from aqueous extract (mixture of 100 g of material with distilled water up to volume of 1000 ml) using an InoLab pH 730 pH meter (WTW, Germany). Lactic acid, acetic acid, and butyric acid were analyzed according to Kvasnicka (2000) on an Ionosep 2003 analyser (RECMAN -laboratory equipment, Czech Republic). Titratable acidity (TA) of aqueous extract was detected by alkalimetric titration to pH 8.5 with 0.1 M potassium hydroxide in the presence of formaldehyde.…”

Section: Methodsmentioning

confidence: 99%

Effects of biological and chemical additives on fermentation progress in maize silage

Tyrolová¹,

Bartoň²,

Loučka³

2017

Czech J. Anim. Sci.

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Tyrolová Y., Bartoň L., Loučka R. (2017): Effects of biological and chemical additives on fermentation progress in maize silage. Czech J. Anim. Sci., 62, 306-312.The objective of this study was to evaluate the effects of bacterial and chemical additives on the number of lactic acid bacteria (LAB) and on fermentation indicators in whole maize silage at 1, 3, 5, 10, and 90 days of fermentation. Maize forage was harvested at approximately 34% dry matter (DM) and treated with (1) no additive (control; C); (2) bacterial inoculant (2 g/t of forage; B) containing the homofermentative LAB Lactobacillus plantarum, Lactobacillus paracasei, and Pediococcus pentosaceus (1.5 × 10 11 cfu/g of inoculant); and (3) chemical additive (4 l/t of forage; CH) containing formic acid, propionic acid, ammonium formate, and benzoic acid. Both treatments decreased pH of silage at day 1 of ensiling (P < 0.05), and the lowest value of 4.34 was observed in the CH-treated silage. All silages were well fermented and had pH < 4.0 by day 10 of fermentation. The concentration of lactic acid and the lactic acid : acetic acid ratio increased over time in all treatment groups, and the highest values were 87.5 and 3.62 g/kg of DM, respectively, observed for group B at day 90 (P < 0.05). The concentrations of water-soluble carbohydrates were higher (P < 0.05) for CH compared to C and B at days 3, 5, 10, and 90 of fermentation. The CH silage had fewer LAB (P < 0.05) than did either C or B silages regardless of the days of fermentation. Both additives used in the present study improved fermentation dynamics of the whole crop maize silage.

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Section: Methodsmentioning

confidence: 99%

Effects of biological and chemical additives on fermentation progress in maize silage

Tyrolová¹,

Bartoň²,

Loučka³

2017

Czech J. Anim. Sci.

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“…At the opening the silages were analyzed for fermentation quality, i.e. pH (measured with a pH meter, WTW Level 1) and concentration of lactic, acetic and butyric acids, determined by isotachophoresis (Kvasnička, 2000) using an Ionosep 2001 apparatus (RECMANLaboratory Systems, Ostrava, Czech Republic). Isotachophoresis is one of the basic electrophoretic techniques used based on injecting a sample solution between leading and terminating solutions present in the separation compartment.…”

Section: Methodsmentioning

confidence: 99%

“…The terminating electrolyte solution contains an anion or cation of a lower effective mobility. The electric field was applied to separate the sample constituents (Kvasnička, 2000). The silage samples were subsequently oven-dried at 50 °C for 48 h and milled to pass a 1 mm screen.…”

Section: Methodsmentioning

confidence: 99%

Comparison of analyses to predict ruminal fibre degradability and indigestible fibre in temperate grass silages

Jančík¹,

Koukolová²,

Homolka³

et al. 2011

SA J. An. Sci.

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________________________________________________________________________________ AbstractThe objective of this study was to compare the ruminal degradability of neutral detergent fibre (NDF) and indigestible NDF (INDF) between silages (n = 24) that originated from three different temperate grass species, i.e. Dactylis glomerata L., Festuca arundinacea L. and hybrid, Felina -Lolium multiflorum L. × Festuca arundinacea S. The data is used to create prediction equations for the effective degradability of NDF (ED NDF ), assuming ruminal solid outflow rates of 0.02 (low), 0.05 (medium) and 0.08/h (high), and INDF. The highest values for the potentially degradable fraction of NDF (b), ED NDF2 , ED NDF5 and ED NDF8 (874, 560, 366 and 272 g/kg NDF, respectively) and the lowest for INDF (73.1 g/kg DM) were found for F. arundinacea. These parameters were significantly different from the hybrid Felina, which presented the lowest rate of NDF degradation (c), ED NDF5 and ED NDF8 (0.033/h, 341 and 250 g/kg NDF, respectively). The highest for c (0.038/h) and INDF (86.9 g/kg DM) and the lowest for b (847 g/kg NDF) were detected for D. glomerata. The combination of acid detergent fibre (ADF) and acid detergent lignin (ADL) (R 2 = 0.844) were identified by a backward, stepwise, multiple regression analysis as the most accurate to predict INDF. Crude protein, ADF and NDF were found as the most suitable combination for predicting ED NDF2 (R 2 = 0.715), ED NDF5 (R 2 = 0.669) and ED NDF8 (R 2 = 0.648). Calculated equations found practical application in laboratory analyses to evaluate the nutritional quality of feeds for ruminants. ________________________________________________________________________________

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“…Silage pH was measured in 100 g of fresh silage diluted in 1000 ml demineralised water with 2 ml toluene added using an InoLab pH 730 pH meter (WTW GmbH, Weilheim, Germany). Lactic acid, acetic acid, propionic acid and butyric acid were analysed according to Kvasnička (2000) by an Ionosep 2003 analyser (RECMAN -laboratory equipment, Ostrava, Czech Republic). Water-soluble carbohydrate (WSC) content was determined according to the Luff Schoorl EEC official method (AOAC, 2005), and ammonia was analysed spectrophotometrically by Libra S 22 (Biochrom Ltd., Cambridge, UK) using Nessler's reagent (AOAC, 2005).…”

Section: Methodsmentioning

confidence: 99%

The effects of wilting and biological and chemical additives on the fermentation process in field pea silage

Tyrolová¹,

Výborná²

2011

CZECH J ANIM SCI

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ABSTRACT:The objectives of the study were to evaluate the effects of wilting and additives on the fermentation quality of field pea silage, and to determine the rumen degradability of organic matter of pea silage. The following additives were used: commercial bacterial inoculant (1 g/t) containing homofermentative lactic acid bacteria -Lactobacillus rhamnosus (NCIMB 30121) and Enterococcus faecium (NCIMB 30122) and chemical additive containing formic acid, propionic acid, ammonium formate and benzoic acid (4 l/t). Compared to the control and chemical additive, the addition of the inoculant to wilted silage increased the lactic acid content (P < 0.05) and lactic:acetic ratio (P < 0.001). Both bacterial and chemical additives decreased (P < 0.001) the pH value of wilted silage. Differences between the control and chemically treated unwilted silage were also significant (P < 0.01). The pH value of silage with chemical additive was lower compared to the control. Proteolysis determined in wilted silage was lower compared to unwilted silage. Rumen degradability of organic matter in wilted silage treated with the chemical additive was found to be higher (P < 0.05) than in control and inoculant treated silages.

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Application of isotachophoresis in food analysis

Abstract: This review summarizes possibilities of capillary isotachophoresis for the determination of important analytes in food. The emphasis is on quantitative determinations in real food samples. This article covers papers published from 1980 to 1999.

Cited by 35 publications

References 49 publications

Effects of biological and chemical additives on fermentation progress in maize silage

Effects of biological and chemical additives on fermentation progress in maize silage

Comparison of analyses to predict ruminal fibre degradability and indigestible fibre in temperate grass silages

The effects of wilting and biological and chemical additives on the fermentation process in field pea silage

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