Application of lanthanum stearate monolayers as a metal-affinity sorbent for the selective sorption of soman adducts to human serum albumin

Babakov, Vladimir; Shreiner, E. V.; Keltsieva, Olga A.; Dubrovskii, Yaroslav A.; Shilovskikh, Vladimir V.; Зорин, И. М.; Суходолов, Н. Г.; Зенкевич, И. Г.; Podolskaya, E.P.; Selyutin, A. A.

doi:10.1016/j.talanta.2018.11.103

Cited by 11 publications

(8 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In general, it gives access to unique structures, characterized with a high regularity at the molecular level [ 26 ]. This can be exemplified by formation of monolayers, accompanying the reaction of stearic acid with a trivalent metal salt (e.g., lanthanum trinitrate) at the interface of organic and aqueous phases [ 23 ]. Such monolayers are well-accessible under standard laboratory conditions.…”

Section: Experimental Designmentioning

confidence: 99%

“…These bottlenecks of GC-MS- and LC-MS-based approaches for FA analysis can be efficiently overcome by implementation of matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF-MS) in combination with Langmuir–Blodgett technology [ 21 ]. Recently, we have shown that Langmuir–Blodgett films can be not only efficiently implemented in selective enrichment of phosphopeptides [ 22 ], protein adducts of organophosphorous compounds [ 23 ] and chlorinated insecticides [ 24 ], but also proved to be an efficient tool for high-throughput and sensitive fingerprinting of free fatty acids as their barium monocarboxylates in positive ion mode [ 25 ]. Therefore, here we implement this approach in a newly developed protocol for the analysis of FFA composition in rhizobial bacteria and propose a comprehensive quantitative solution for fingerprinting of FFAs in cultured cells by MALDI-TOF-MS.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

High-Throughput Fingerprinting of Rhizobial Free Fatty Acids by Chemical Thin-Film Deposition and Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry

Gladchuk

Shumilina

Kusnetsova

et al. 2020

MPs

View full text Add to dashboard Cite

Fatty acids (FAs) represent an important class of metabolites, impacting on membrane building blocks and signaling compounds in cellular regulatory networks. In nature, prokaryotes are characterized with the most impressing FA structural diversity and the highest relative content of free fatty acids (FFAs). In this context, nitrogen-fixing bacteria (order Rhizobiales), the symbionts of legumes, are particularly interesting. Indeed, the FA profiles influence the structure of rhizobial nodulation factors, required for successful infection of plant root. Although FA patterns can be assessed by gas chromatography—(GC-) and liquid chromatography—mass spectrometry (LC-MS), sample preparation for these methods is time-consuming and quantification suffers from compromised sensitivity, low stability of derivatives and artifacts. In contrast, matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF-MS) represents an excellent platform for high-efficient metabolite fingerprinting, also applicable to FFAs. Therefore, here we propose a simple and straightforward protocol for high-throughput relative quantification of FFAs in rhizobia by combination of Langmuir technology and MALDI-TOF-MS featuring a high sensitivity, accuracy and precision of quantification. We describe a step-by-step procedure comprising rhizobia culturing, pre-cleaning, extraction, sample preparation, mass spectrometric analysis, data processing and post-processing. As a case study, a comparison of the FFA metabolomes of two rhizobia species—Rhizobium leguminosarum and Sinorhizobium meliloti, demonstrates the analytical potential of the protocol.

show abstract

Section: Experimental Designmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

High-Throughput Fingerprinting of Rhizobial Free Fatty Acids by Chemical Thin-Film Deposition and Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry

Gladchuk

Shumilina

Kusnetsova

et al. 2020

MPs

View full text Add to dashboard Cite

show abstract

“…These bottle necks of GC-MS-and LC-MS-based approaches for FA analysis can be efficiently overcome by implementation of matrix-assisted laser desorption/ionization time-of flight mass spectrometry (MALDI-TOF-MS) in combination with Langmuir-Blodgett technology [21]. Recently, we have shown that Langmuir-Blodgett films can be not only efficiently implemented in selective enrichment of phosphopeptides [22], protein adducts of organophosphorous compounds [23] and chlorinated insecticides [24], but also proved to be an efficient tool for high-throughput and sensitive fingerprinting of free fatty acids as their barium monocarboxylates in positive ion mode [25]. Therefore, here we extend this approach to analysis of FFA composition of rhizobial bacteria and propose a comprehensive protocol for fingerprinting of FFAs in cultured cells by MALDI-TOF-MS.…”

Section: Figure 1 Structural Class Of Bacterial Fatty Acidsmentioning

confidence: 99%

“…This can be exemplified by formation of monolayers, accompanying the reaction of stearic acid with a trivalent metal salt (e.g. lanthanium trinitrate) at the interface of organic and aqueous phases [23]. Such monolayers are well-accessible under standard laboratory conditions.…”

Section: Experimental Designmentioning

confidence: 99%

A High-throughput Method for Fingerprinting of Rhizobial Free Fatty Acids by the Combination of a Thin Film Chemical Deposition Technique and Matrix-assisted Laser Desorption/ionization Time-of Flight Mass Spectrometry

Gladchuk¹,

Schumilina²,

Kusnetsova³

et al. 2020

Preprint

View full text Add to dashboard Cite

Fatty acids (FAs) represent an important class of metabolites, impacting on membrane building blocks and cellular regulatory networks. In nature, prokaryotes are characterized with the most impressing FA structural diversity and the highest relative contents of free fatty acids (FFAs). Thereby, nitrogen-fixing bacteria (order Rhizobiales), often found in symbiosis with legumes, attract a special interest. Indeed, FAs impact on the structure of rhizobial nodulation factors, required for successful infection of plant root. Although the FA patterns can be addressed by GC- and LC-MS, these methods are time-consuming and suffer from compromised sensitivity, low stability of derivatives and artifacts. In contrast, MALDI-TOF-MS represents an excellent platform for high-efficient metabolite fingerprinting, also applicable to FFAs. Therefore, here we propose a simple and straightforward protocol for high-throughput relative quantification of FFAs in rhizobia by the combination of Langmuir technology and MALDI-TOF-MS, which is featured with high sensitivity, accuracy and precision of quantification. Here we propose a step by step procedure comprising rhizobia culturing, pre-cleaning, extraction, sample preparation, mass spectrometric analysis, data processing and post-processing. To demonstrate the analytical potential of the protocol we illustrate it by a case study – comparison the FFA metabolomes of two rhizobia species - Rhizobium leguminosarum and Sinorhizobium meliloti.

show abstract

“…The main advantages of optical polymer fibers in comparison with glass are low weight, low price, and ease of use. , Apropos, recent works have appeared in which monolayers containing ions of transition metals and lanthanides are used as sorption materials. In particular, these works investigate similar materials as adsorbents for bioorganic analysis. − Thin films transferred onto a substrate can act as metal-affinity sorbents. Such sorbents have an advantage because metals with an increased affinity for sites of biologically active macromolecules are accessible.…”

Section: Introductionmentioning

confidence: 99%

Thin Films of Lanthanide Stearates as Modifiers of the Q-Sense Device Sensor for Studying Insulin Adsorption

et al. 2022

View full text Add to dashboard Cite

This article presents new possibilities of using thin films of lanthanide stearates as sorbent materials. Modification of the Q-sense device resonator with monolayers of lanthanide stearates by the Langmuir–Schaeffer method made it possible to study the process of insulin protein adsorption on the surface of new thin-film sorbents. The resulting films were also characterized by compression isotherms, chemical analysis, scanning electron microscopy, and mass spectrometry. The transition of stearic acid to salt was recorded by IR spectroscopy. Using the LDI MS method, the main component of thin films, lanthanide distearate, was established. The presence of Eu 2+ in thin films was revealed. In the case of europium stearate, the maximum value of insulin adsorption was obtained, −1.67·10 −10 mole/cm 2 . The findings suggest the possibility of using thin films of lanthanide stearates as a sorption material for the proteomics determination of the quantitative protein content in complex fluid systems by specific adsorption on modified surfaces and isolation of such proteins from complex mixtures.

show abstract

Application of lanthanum stearate monolayers as a metal-affinity sorbent for the selective sorption of soman adducts to human serum albumin

Cited by 11 publications

References 19 publications

High-Throughput Fingerprinting of Rhizobial Free Fatty Acids by Chemical Thin-Film Deposition and Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry

High-Throughput Fingerprinting of Rhizobial Free Fatty Acids by Chemical Thin-Film Deposition and Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry

A High-throughput Method for Fingerprinting of Rhizobial Free Fatty Acids by the Combination of a Thin Film Chemical Deposition Technique and Matrix-assisted Laser Desorption/ionization Time-of Flight Mass Spectrometry

Thin Films of Lanthanide Stearates as Modifiers of the Q-Sense Device Sensor for Studying Insulin Adsorption

Contact Info

Product

Resources

About