Application of laser scanning speckle-microscopy for high-resolution express diagnostics of chlamydial infection

Amplification of speckle-microscope signal by using gold nanoparticle stable of contents

“…Specific output characteristics of speckle-microscope with the implication to the scanning of C. trachomatis bacteria inclusions inside the human epithelial cells have been analyzed (27). In the citing papers (27,28), it has been shown that: (i) in the scanning of a thin sample of cellular suspension fixed between two glasses the influence of Brownian motion of CT Elementary Bodies (here as CT cells) on the formation of output signal of speckle-microscopy is negligible and (ii) the signal from scanning CT cells can be reliably and stably detected. But signal-tonoise ratio of output signal of speckle-microscope is not so high and the useful part of output signal generated by scattering of light from CT cell should be enhanced.…”

“…General output characteristics of speckle microscope have been studied (26). It is important to note that very recently speckle-microscopy has been applied (27)(28)(29) in the investigation of a unique biological object -Chlamydia trachomatis (CT), the causative agent of chlamydial genital infection that is known as one of the greatest challenges for both Public Human and Animal Health worldwide. A specific feature of these bacterial cells is that they can live only inside other mammalian cells of larger size, for example epithelial cells.…”