Application of LC-DAD Metabolic Fingerprinting in Combination with PCA for Evaluation of Seasonality and Extraction Method on the Chemical Composition of Accessions from Lippia alba (Mill) N. E. Brown and Biological Activities

Jesus, Raphael Amancio de; Prado, Vilma M. J.; Pinto, Vanderson dos Santos; Silva, Valdenizia R.; Santos, Luciano Soares dos; Nogueira, Paulo C.L.; Navickiene, Sandro; Pereira-Filho, Edenir Rodrigues; Blank, Arie Fitzgerald; Bezerra, Daniel P.; Soares, Milena Botelho Pereira; Seidl, Claudia; Cardoso, Carmen L.; Moraes, Valéria R. S.

doi:10.21577/0103-5053.20180244

Cited by 4 publications

(6 citation statements)

References 28 publications

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“…On-flow immobilized acetylcholinesterase inhibition studies -Activity and inhibition studies using immobilized acetylcholinesterase from Electrophorus electricus (electric eel) were performed as described by Jesus et al (2019). Stock solutions (5 mg mL −1 ) of each sample were prepared in the appropriate solvents, depending on the method of extraction, as follows: ultrapure water (decoction and infusion), 50% ethanol/water (hydroethanolic solution), methanol (methanolic extracts and methanol partitions), methanol (ethanolic extracts), and hexane (hexane partitions).…”

Section: Methodsmentioning

confidence: 99%

Biological studies and chromatograms aided by chemometric analysis in evaluation of seasonality and extraction method of Croton grewioides extracts

et al. 2022

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The genus Croton is the second largest genus of Euphorbiaceae family, comprising more than 1300 registered species. The species Croton grewioides is endemic in the northeast of Brazil and distributed mainly throughout the caatinga biome presenting, in its essential oils, various compounds with biological activities such as pupicidal, larvicidal, insecticidal, and bactericidal. The secondary metabolites commonly found in their extracts include diterpenes, phenols, steroids, and aglycone and glycoside flavonoids derivatives, which can undergo variations based on climatic changes that modify processes such as photosynthesis, transpiration and nutrient cycle. To understand the effects of seasonal variability and extraction method on the biological activities of polar extracts from C. grewioides, fingerprint chromatograms were analyzed aided by chemometric analysis. In vitro assays of cytotoxicity against HL-60 and HepG2 cancer cells and anti-acetylcholinesterase activity were performed. Seasonal effects could only be observed in the chemical profiles of the methanolic and ethanolic extracts from accessions 113 and 126, respectively. Considering the extraction method, the chromatograms of aqueous and hydroalcoholic extracts of all the accessions were similar, but differed from those for the corresponding methanolic and ethanolic extracts. Five hexane partitions were active against HL-60 cell line, displaying inhibition values between 81 and 93%. Only the ethanolic extract of accession 101 (winter), the methanolic partition from the methanolic extract, and the decoction of accession 113 (winter) presented high inhibition values against the acetylcholinesterase enzyme. This study enabled elucidation of the effects of seasonality and extraction method on the biological activities presented by the C. grewioides extracts.

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Section: Methodsmentioning

confidence: 99%

Biological studies and chromatograms aided by chemometric analysis in evaluation of seasonality and extraction method of Croton grewioides extracts

et al. 2022

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“…The aqueous extracts prepared by infusion were obtained following the methodology described by Jesus et al, 14 with some modifications.…”

Section: Infusion Extractionmentioning

confidence: 99%

“…[6][7][8][9][10][11][12] The main sources of phenolic compounds include medicinal plants traditionally used in countries all over the world for the treatment of diseases. 4,13,14 Previous studies carried out with species of the genus Lantana (family Verbenaceae) have highlighted their great chemical diversity and their important biological and economic potentials. 7,15,16 The species Lantana camara, known in Brazil as camará-de-cheiro, is used in folk medicine for the treatment of measles, stomach pain, tumors, rheumatism, and bronchitis.…”

Section: Introductionmentioning

confidence: 99%

“…The main sources of phenolic compounds include medicinal plants traditionally used in countries all over the world for the treatment of diseases 4,13,14 . Previous studies carried out with species of the genus Lantana (family Verbenaceae) have highlighted their great chemical diversity and their important biological and economic potentials 7,15,16 …”

Section: Introductionmentioning

confidence: 99%

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Microwave and ultrasound extraction of antioxidant phenolic compounds from Lantana camara Linn. leaves: Optimization, comparative study, and FT‐Orbitrap MS analysis

de Jesus,

da Silva,

Wisniewski

et al. 2024

Phytochemical Analysis

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IntroductionThe species Lantana camara is used in folk medicine. The biological activities of this medicinal plant are attributable to the presence of various derivatives of triterpenoids and phenolic compounds present in its preparations, indicating excellent economic potential.ObjectiveIn this study, the operational conditions of ultrasound‐assisted extraction (UAE) and microwave‐assisted extraction (MAE) were optimized using Box–Behnken design to improve the total phenolic content (TPC) recovered in hydroethanolic extracts of L. camara leaves.Material and methodsThe TPC, total flavonoid content (TFC), and antioxidant activities of the hydroalcoholic extracts of L. camara, prepared by UAE and MAE under the optimized extraction conditions, were compared with those of the extracts obtained by conventional extraction methods.ResultsUnder the optimal conditions, the extracts obtained by UAE (35% ethanol, 25 min, and a solvent‐to‐solid ratio of 60:1 mL/g) and by MAE (53% ethanol, 15 min, and 300 W) provided high yields of 32.50% and 38.61% and TPC values of 102.89 and 109.83 mg GAE/g DW, respectively. The MAE extract showed the best results with respect to TPC, TFC, and antioxidant activities, followed by extracts obtained by UAE, Soxhlet extraction, decoction, maceration, and infusion, in that order.ConclusionThe results obtained indicate that L. camara may be used as an important source of antioxidant phenolic compounds to obtain products with high biological and economic potential, especially when the extraction process is performed under appropriate conditions using MAE and/or UAE, employing environmentally friendly solvents such as water and ethanol.

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“…In addition, statistical analysis of the complex data obtained is also a powerful tool to the metabolomics field. 10,11 The objective of this work was to study the metabolomic differences of ucuuba (Virola surinamensis) raw seed butter from three distinct Amazonian regions in order to characterize the chemical differences between samples of the same species provided from different environments. Advanced analytical techniques (GC-FID and UPLC-ESI-QToF-MS) were used to provide information about primary and secondary metabolites present in the samples.…”

mentioning

confidence: 99%

Metabolomic study of Virola surinamensis (Rol.) Warb raw seed butter from different Amazonian regions

Takano¹,

Reigada²,

Domenico³

et al. 2019

Int J Phytocos Nat Ingred

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Introduction Ucuuba is a tupi denomination that means "tree that produces fatty". This designation is usually applied in the Brazilian Amazon for most species of the botanical genus Virola. 1 Virola surinamensis (Rol.) Warb, known as ucuuba, is considered as a typically Amazonian species and its habitat is the lowlands and igapós. The population thrives in flooded areas, river banks, igarapés and furos, or areas that might be reached by river flooding. 2 Thriving species in this environment are açaí, murumuru, patawa and buriti palms, and ucuuba, samaúba, munguba and rubber trees. It is considered as an important biological, social and economic resource for Amazon, as a key species for biological maintenance of flooded areas, due to its environmental interrelations. 3 In the 18th and 19th centuries, butter extracted from ucuuba seeds was initially used as a lighting fuel by Amazonian riverside communities. 4 However, it was in the 1970s and 1980s that the tree was intensely explored for national wood production. Until 1998, ucuuba was the third most exported and commercialized species, representing almost 50% of riverside communities' family income. 5 This factor led ucuuba to be in the international endangered species list of IUCN. 6 Despite the legal measures in Brazil, in the 1990s and the beginning of 2000s, ucuuba was still the third most exported tree species for the international market of tropical woods. 7 Currently, the species is once more in the spotlight, due to the non-timber potential. Butter extracted from the seeds has high moisturizing potential and, at the same time, a soft texture, rapid absorption and velvet dry touch. These characteristics make the ucuuba butter an interesting ingredient for cosmetic formulations.

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Application of LC-DAD Metabolic Fingerprinting in Combination with PCA for Evaluation of Seasonality and Extraction Method on the Chemical Composition of Accessions from Lippia alba (Mill) N. E. Brown and Biological Activities

Cited by 4 publications

References 28 publications

Biological studies and chromatograms aided by chemometric analysis in evaluation of seasonality and extraction method of Croton grewioides extracts

Biological studies and chromatograms aided by chemometric analysis in evaluation of seasonality and extraction method of Croton grewioides extracts

Microwave and ultrasound extraction of antioxidant phenolic compounds from Lantana camara Linn. leaves: Optimization, comparative study, and FT‐Orbitrap MS analysis

Metabolomic study of Virola surinamensis (Rol.) Warb raw seed butter from different Amazonian regions

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