Application of Liquid-Based Preparation to Fine Needle Aspiration Cytology in Breast Cancer

Komatsu, Kyoko; Nakanishi, Yoko; Seki, Toshimi; Yoshino, Atsuko; Fuchinoue, Fumi; Amano, Sadao; Komatsu, Akio; Sugitani, Masahiko; Nemoto, Norimichi

doi:10.1159/000325603

Cited by 26 publications

(39 citation statements)

References 9 publications

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“…Studies have shown that cellular aggregates are fragmented, shortened and less distinct in LBC preparations, resulting in smaller clusters and cellular dissociation [7,10,17]. In another study, even though cellular cohesiveness was not altered, a three-dimensional arrangement was found to be more pronounced in both benign and malignant breast lesions [16].…”

Section: General Cytomorphological Characteristicsmentioning

confidence: 99%

Liquid-Based Cytology in Fine-Needle Aspiration of Breast Lesions: A Review

Gerhard

Schmitt²

2014

Acta Cytologica

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Objective: Fine-needle aspiration (FNA) is a safe and cost-effective technique for the diagnosis of breast lesions, especially when correlated with clinical and imaging studies. However, the success of breast FNA is highly dependent on the adequate preparation of cytological conventional smears (CS). The liquid-based cytology (LBC) technique consists of an automated method for preparing thin-layer cytological samples from cell suspensions collected in alcohol-based preservative. LBC is designed to improve CS by avoiding limiting factors such as obscuring material, air-drying and smearing artifacts. Study Design: We performed a review of the published literature about LBC applied to breast FNA. Results: LBC preparations of breast aspirates demonstrated better cellular preservation, less cell overlapping and elimination of blood and excessive inflammation compared to CS. Conversely, alterations in architecture and cell morphology as well as loss of myoepithelial cells and stromal elements have been described in LBC specimens, requiring training before applying this technique for diagnosis. Studies have shown a similar accuracy between LBC and CS for the diagnosis of breast lesions. LBC also permits the use of residual material for ancillary tests, which is an important advantage compared to CS. Conclusions: LBC can be safely applied to breast FNA, showing a similar diagnostic accuracy to CS.

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Section: General Cytomorphological Characteristicsmentioning

confidence: 99%

Liquid-Based Cytology in Fine-Needle Aspiration of Breast Lesions: A Review

Gerhard

Schmitt²

2014

Acta Cytologica

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“…At present, the two most commonly used liquid-based sampling techniques are ThinPrep (TP; CYTYC Co., Marlborough, Mass., USA) and SurePath® (SP; Tripath PREP, BD SurePath Inc., Burlington, N.C., USA). Most studies of liquid-based cytology have focused on TP methodology [11,12,13,14,15,16], and, to our knowledge, there are no studies concerning cytomorphological features on breast FNAC using the SP technique. …”

Section: Introductionmentioning

confidence: 99%

A Pilot Study Evaluating Liquid-Based Fine Needle Aspiration Cytology of Breast Lesions: A Cytomorphological Comparison of SurePath® Liquid-Based Preparations and Conventional Smears

Ryu

Park

et al. 2013

Acta Cytologica

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Objective: Fine needle aspiration cytology (FNAC) is recommended by the World Health Organization as a diagnostic method for breast lesions. The morphological interpretation of liquid-based preparations (LBPs) remains a diagnostic challenge due to considerably altered cytomorphology. The aim of the current study was to compare cytomorphological characteristics of SurePath® (SP)-based LBP and conventional smear (CS) in breast FNACs. Study Design: The study included 77 benign and 60 malignant breast FNACs obtained by both SP and CS, all with tissue confirmation. Cases analyzed with both preparations were reviewed and compared, focusing on 10 cytomorphological features. Results: SP aspirates demonstrated notable cytomorphological alterations. Among them, a prominent three-dimensional configuration of cell clusters and frequent and conspicuous nucleoli were the most prominent characteristics of SP compared with CS. Overall diagnostic performances were comparable but were slightly lower for SP than CS (diagnostic accuracy of two reviewers; 87.6 and 90.5% for SP vs. 91.2 and 92.7% for CS, respectively). Conclusion: Although the reviewer should be aware of distinctive cytomorphological alterations, the SP technique is reliable for the evaluation of breast lesions with the advantage of easy interpretation and a diagnostic accuracy equivalent to CS.

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“…Liquid-based cytology in breast FNA had a good correlation with conventional preparation of breast FNA with the advantages of easier and less time consuming evaluation of cell morphology (clear background, no overlapping, smaller area to screen), reproducibility, a factor of great importance to quality control; and possibility of adjunctive investigations such as immunocytology and flow cytometry on the same material [11]. This has been corroborated with the addition that quantitative analysis of HER-2 mRNA correlated with the results of immunohistochemistry in cancer patients [12]. We have found similar morphological characteristics in our study with the ability to produce multiple clean smears from a single needle containing cells from breast specimen.…”

Section: Discussionmentioning

confidence: 65%