Application of liquid chromatography–tandem mass spectrometry for the determination of opioidmimetics in the brain dialysates from rats treated with opioidmimetics intraperitoneally

Developmental Potential for Endomorphin Opioidmimetic Drugs

“…These results demonstrated that compound 57 crossed epithelial membrane barriers in both the intestine and microcapillaries in mouse brain to interact with brain μ -opioid receptors. Similar conclusions were obtained by Igarashi et al [ 31 ] and Koda et al [ 32 ]. These results indicated that pyrazinone derivatives could be potential candidates for clinical and therapeutic applications in the treatment of pain arising from postoperative procedure or cancer, associated with birth, or act as possible veterinary drugs.…”

“…The extraordinary dual δ / μ -antagonism of 69 and 70 qualifies these compounds as potential pharmacological tools for application in the clinical and therapeutic treatment of drug addiction and alcohol dependency. Considering that the bis -Dmt analogues containing alkylpyrazinone are orally active and pass through the blood-brain barrier [ 19 , 31 , 32 ], we would anticipate that 69 and 70 might show similar properties or may be even more potent due to their increased hydrophobicity [ 35 ].…”

A review of sample preparation methods for quantitation of small-molecule analytes in brain tissue by liquid chromatography tandem mass spectrometry (LC-MS/MS)

“…Ringer's solution is one of the most widely used perfusates in microdialysis, which is an aqueous solution containing 149 mM NaCl, 3.0 mM KCl, 1.2 mM CaCl 2 , and 0.8 mM MgCl 2 with the pH adjusted to 7.4. 99,100,102,103,108,109,113,115,116,118,119,[121][122][123] Articial cerebrospinal uid (aCSF) is another commonly used perfusate in brain tissue microdialysis. 101,104,107,110,114,117,120,124 aCSF may have different formulas in different studies, but in general it is an isotonic aqueous solution that is close to the composition and pH of physiological CSF.…”

“…Another way to fulll online desalting is by using column-switching, which will be discussed in the LC chapter. 77,102,103,108,116,123 When instrumentation is limited, desalting can also be fullled by offline techniques. In a study involving the quantitation of dopamine and its metabolites in brain microdialysates, Syslová et al developed an offline desalting method using lyophilization.…”

Quantitative Analysis of Chloramphenicol in Royal Jelly by Column-switching LC-MS/MS Using a Pretreatment Column with a Higher-pressure Capability

“…Among pretreatment columns, the methylcellulose-immobilized reversed-phase (MC-ODS) column showed excellent performance for drug or chemical analysis of biological samples [1][2][3][4][5][6][7][8] and compounds in the environment. 9 While small molecules, such as drugs and environmental contaminants, enter in and are retained on the reversed-phase inner surface of small pores (12 nm), large molecules, such as proteins are excluded.…”