Application of low‐wavenumber Raman spectroscopy to the analysis of human teeth

Abstract

Low‐wavenumber Raman spectroscopy is demonstrated for its potential to analyse mineralization abnormalities in human teeth. Four different dental samples were analysed in this study, denoted as healthy control, mild hypomineralization, enamel caries, and AI (severely degraded due to Amelogenesis imperfecta). All specimens were imaged using principal component analysis. The spectra from low‐ wavenumber and midwavenumber regions were simultaneously collected allowing for direct comparison between the two regions. This approach provided a method to characterize structural and chemical variation within samples. Structurally different hydroxyapatite was found to be present in the surface and bulk of the enamel. The thin outer layer of the enamel contained the most structurally ordered mineral. The obtained Raman images enabled characterization and comparison of lesion domains as well as relative hydroxyapatite abundances across all dental samples.

Raman and XANES Spectroscopic Study of the Influence of Coordination Atomic and Molecular Environments in Biomimetic Composite Materials Integrated with Dental Tissue

“…The comparison was made based on data taken from the literature. Analysis of the results shows that the main and most intense modes in the spectra of the natural tissues and the synthesised biocomposites can be attributed to the characteristic vibrations of cHAp (see Table 2), which is the basis of the mineral component of both the natural tissues and the biomimetic composites [32,40,43,[46][47][48].…”

“…2− [40,43,[46][47][48][49]. Figure 3 (centre and left) shows the spectral regions where the υ 2 and υ 4 PO 4 bending modes are located.…”

“…These peaks have the lowest width in the spectrum of stoichiometric HAp (sample H S ). It is known [32,33,40,46,53,54] that the increased content of carbonate groups in the hydroxyapatite structure causes the broadening of all bands in its Raman spectrum, resulting in the appearance of a wide band at around 1070 cm −1 with poorly resolved shoulders. This phenomenon is represented in the spectra of the reference samples of nano-cHAp, natural tissues and biocomposites (Figure 4).…”

Qualitative and quantitative vibrational spectroscopic analysis of macronutrients in breast milk

“…Over the years, vibrational spectroscopic techniques such as Raman and mid-infrared (IR) spectroscopy have been increasingly used for biomedical and biological applications [ [1] , [2] , [3] ]. These methods are capable of providing information related to the molecular structure and chemical composition as they probe vibrational motions, which are highly sensitive to changes of these properties [ 4 ].…”

Principal Component Regression for Forensic Age Determination Using the Raman Spectra of Teeth

“…4 For example, biochemical analyses require very small amounts of mineralized tissues and are applicable in cases of extensively damaged teeth. 5 Raman spectrometric analyses of dental hard tissues have been performed in various studies, including evaluations of enamel mineralization, [6][7][8] early caries detection, [9][10][11] compositional differences between primary and permanent teeth, 12 and mapping of the spatial distribution of organic and inorganic components in dental tissues. 13 However, only two studies have employed Raman spectrometry to relate the aging-dependent compositional changes in tooth tissues with the donor's age for forensic purposes.…”