Application of Magnetic Agarose Support in Liquid Magnetically Stabilized Fluidized Bed for Protein Adsorption

Abstract

A novel magnetic agarose support (MAS) was fabricated for application in a liquid magnetically stabilized fluidized bed (MSFB). It was produced by water-in-oil emulsification method using a mixture of agarose solution and nanometer-sized superparamagnetic Fe(3)O(4) particles as the aqueous phase. The MAS showed good superparamagnetic responsiveness in a magnetic field. A reactive triazine dye, Cibacron blue 3GA (CB), was coupled to the gel to prepare a CB-modified magnetic agarose support (CB-MAS) for protein adsorption. Lysozyme was used as a model protein to test the adsorption equilibrium and kinetic behavior of the CB-MAS. The dependence of bed expansion in the MSFB with a transverse magnetic field on liquid velocity and magnetic field intensity was investigated. Liquid-phase dispersion behavior in the MSFB was examined by measurements of residence time distributions and compared with that obtained in packed and expanded beds. Dynamic lysozyme adsorption in the MSFB was also compared with those in packed and expanded beds. The dynamic binding capacity at 10% breakthrough was estimated at 55.8 mg/mL in the MSFB, higher than that in the expanded bed (31.1 mg/mL) at a liquid velocity of 45 cm/h. The results indicate that the CB-MAS is promising for use in liquid MSFB for protein adsorption.

Separation of lysozyme with magnetically stabilized spherical hydroxyapatite microcomposites in a continuous flow system

“…Figure 2 shows the FTIR spectrum of the pure HA, m-s HA microcomposites, and Fe 3 O 4 nanopowder. In Figure 2(a), the peaks at 602, 962, and 1035 cm 21 are typical frequencies of the PO 4 groups of HA, and also, the sharp band at 3500 cm 21 shows AOH stretching. 28,29 When the m-s HA microcomposites' FTIR spectra were compared with those of pure HA and Fe 3 O 4 , the modifications could be clearly seen.…”

“…With the incorporation of EGDMA in the structure, extra peaks between 1600 and 1750 cm 21 because of to the carbonyl stretching of EGDMA. In addition, there were also CAH bending bands in the frequency range of 1200-1400 cm 21 . Figure 3 shows the XRD patterns of the magnetite (Fe 3 O 4 ), pure HA, and porous spherical HA microcomposites.…”

“…In the adsorption medium (50 mM at pH 7.0 in phosphate buffer), the protein surface will be positively charged, and the adsorbent surface will be negatively charged. The presence of phosphate ions will cover the surface of the adsorbent because they will interact with Ca 21 ions. This could eliminate the electrostatic repulsions between the positive groups of protein and Ca 21 ions.…”

“…The presence of phosphate ions will cover the surface of the adsorbent because they will interact with Ca 21 ions. This could eliminate the electrostatic repulsions between the positive groups of protein and Ca 21 ions. At this pH value, therefore, positively charged lysozyme could interact more easily with the m-s HA surface.…”

Magnetic Dye Affinity Beads for the Adsorption of β‐Casein

“…Many kinds of magnetic particles are more commonly manufactured from polymers since they have a variety of surface functional groups which can be tailored for use in for various applications. [21][22][23][24][25][26] Superparamagnetic poly(vinyl acetate-divinyl benzene) microspheres, [27] magnetic agarose supports, [28] phospholipid coated colloidal magnetic nanoparticles [29] and poly(methacrylate-divinyl benzene) microspheres [30] are typical polymeric carriers which can be used in enzyme immobilization studies.…”