Application of Magnetic Core–Shell Imprinted Nanoconjugates for the Analysis of Hordenine in Human Plasma-Preliminary Data on Pharmacokinetic Study after Oral Administration

Abstract

In this paper, we developed and validated a new analytical method to determine the pharmacokinetic profile of hordenine in plasma samples of human volunteers after oral administration of hordenine-rich dietary supplements. For this purpose, a magnetic molecularly imprinted sorbent was fabricated and characterized. The application of a magnetic susceptible material facilitates pretreatment step while working with a highly complex sample, reducing time and costs. An optimized, fast, and reliable separation step was combined with liquid chromatography tandem mass spectrometry, providing an analytical method for analysis of hordenine in human plasma after dietary supplement intake. The method was validated (lower limit of quantification of 0.05 μg/L), enabling the pharmacokinetic profile of hordenine to be determined. The highest concentration of hordenine was noted after 65 ± 14 min, reaching the value of 16.4 ± 7.8 μg/L. The average t 1/2 was 54 ± 19 min. The apparent volume of distribution was 6000 ± 2600 L (66 ± 24 L/kg when adjusted for weight).

Magnetic Core–Shell Molecularly Imprinted Nano-Conjugates for Extraction of Antazoline and Hydroxyantazoline from Human Plasma—Material Characterization, Theoretical Analysis and Pharmacokinetics

“…The magnetic core–shell polymerization process was proceeded similarly to previously described [ 29 ] to prepare imprinted Fe 3 O 4 @SiO 2 -MPS@MIP (coded as mag-MIP) and non-imprinted Fe 3 O 4 @SiO 2 -MPS@NIP (coded as mag-NIP) polymers. For synthesis of mag-MIP, the structural analog of target analyte was used as the template [ 30 ] and for synthesis of mag-NIP the addition of template was omitted.…”

Imprinting Technology for Effective Sorbent Fabrication: Current State-of-Art and Future Prospects

“…Most of the studies in this area focus on the synthesis and characterization of magnetic core-shell materials, but there are also some works devoted to molecularly imprinted stir-bars. Sobiech and co-workers [ 84 ] proposed a magnetic core conjugate with an imprinted shell for sorption of hordenine from human plasma for pharmacokinetic study. Here, the functionalization of magnetic core was proceeded with 3-(trimethoxysilyl)propyl methacrylate prior to the polymerization in the presence of N,N-dimethylphenethylamine, a template, and a structural analog of the target analyte known as hordenine.…”

“… Scanning ( a – e ) and transmission ( f ) electron micrographs, nitrogen sorption data ( g – i ), X-ray diffractogram ( j ), and hysteresis from a vibrating sample magnetometer ( k ) of magnetic imprinted sorbent [ 84 ]. …”

“…Most of the studies in this area focus on the synthesis and characterization of magnetic core-shell materials, but there are also some works devoted to molecularly imprinted stir-bars. Sobiech and co-workers [84] proposed a magnetic core conjugate with an imprinted shell for sorption of hordenine from human plasma for pharmacokinetic study.…”

“…It decreased for silanized core to 7 m 2 g −1 but increased further for a core-imprinted shell to 19 m 2 g −1 (Figure 4g-i). (a-e) and transmission (f) electron micrographs, nitrogen sorption data (g-i), X-ray diffractogram (j), and hysteresis from a vibrating sample magnetometer (k) of magnetic imprinted sorbent [84]. Figure 4.…”