Application of magnetic force‐based cell patterning for controlling cell–cell interactions in angiogenesis

Abstract

To investigate the effects of cell-cell interactions on cellular function, the microenvironment surrounding cells should be precisely controlled. Here, we describe a cell patterning technique, which utilizes magnetic force and magnetite nanoparticles. This method was used to develop cell culture arrays for investigation of cell behaviors in angiogenesis. Pin holder devices that contain more than 6,000 pillars on the surface are used for fabricating the cell culture arrays by setting it on a magnet. The magnetically labeled cells were arranged by magnetic distribution. When the human umbilical vein endothelial cells are arranged at 250 microm intervals (5.9 cells/spot), the cells spread toward other cell cluster on adjacent spots in 4.5 h, and formed cord-like structures in 8.5 h. It was shown that cell-cell interactions were successfully investigated using magnetic cell arrangement.

Genetically encoded force sensors for measuring mechanical forces in proteins

“…[6][7][8] Numerous studies have also indicated that mechanical forces can regulate gene expression. [9][10][11][12] There are feedback loops incorporating cancer malignancy and metastasis, 17,18 blood vessel angiogenesis, [19][20][21][22] bone growth and osteoporosis and lung, heart and skeleton muscle metabolism.…”

Microchemistry- and MEMS-based Integrated Electrochemical Devices for Bioassay Applications

“…18,19 Array patterns of microparticles or cells have been utilized in the design of biosensors, as scaffolds for patterning proteins, and in cell cultivation. In recent years, various techniques for manipulating bioparticles on chips have been developed using hydrodynamics, 1,73 magnetic forces, [74][75][76][77][78][79][80] and DEP. 16,17,81-85 DEP was first described by Pohl as the motion of dielectric particles under the influence of a non-uniform electric filed.…”

Manipulation of microparticles for construction of array patterns by negative dielectrophoresis using multilayered array and grid electrodes

“…The physical manipulation of biological particles is a vital component of miniaturized biotechnological platforms such as ''lab-on-a-chip'' devices and arrays for high-throughput assays. In recent years, many techniques regarding the manipulation of bioparticles on chips have been developed using hydrodynamics (El-Ali et al, 2006;Peterson 2005;Yang et al, 2007), magnetic forces (Ino et al, , 2009Ito et al, 2007Ito et al, , 2005Tsuchiya et al, 2008), dielectrophoresis (DEP) (Albrecht et al, 2006(Albrecht et al, , 2007Bocchi et al, 2009;Hsiung et al, 2008;Kim et al, 2007;Lee et al, 2008;Suzuki et al, 2004Suzuki et al, , 2007Suzuki et al, , 2008Taff and Voldman, 2005;Tornay et al, 2008;Wang et al, 2007;Yantzi et al, 2007;Yasukawa et al, 2007), electrophoresis , and pneumatic force (Hiranishi et al, 2007;Jeong and Konishi, 2009).…”