Application of magnetic hydroxyapatite nanoparticles for solid phase extraction of plasmid DNA

Shan, Zhi; Li, Xianghai; Gao, Yaying; Wang, Xianxiang; Li, Chenglei; Wu, Qi

doi:10.1016/j.ab.2012.03.013

Cited by 31 publications

(10 citation statements)

References 12 publications

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“…The most striking finding of plasmid DNA biodistribution monitoring was the extensive binding of DNA to intact bone, indicating that the applied DNA gets absorbed by bone tissue. Binding of nucleic acids to ceramic hydroxyapatite has been well known already for some time (Kothari and Shankar, 1974;Mazin, 1977) and harnessed for DNA purification (Shan et al, 2012) and delivery (Choi and Murphy, 2010;Zhang et al, 2011). This study, however, is the first study to demonstrate this effect in vivo with biological hydroxyapatite and naked exogenous DNA in orthopedic gene delivery.…”

Section: Orthotopic Bmp2/7 Sonoporationmentioning

confidence: 82%

Sonoporation Increases Therapeutic Efficacy of Inducible and Constitutive BMP2/7 In Vivo Gene Delivery

Feichtinger

Hofmann

Slezak

et al. 2014

Human Gene Therapy Methods

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An ideal novel treatment for bone defects should provide regeneration without autologous or allogenous grafting, exogenous cells, growth factors, or biomaterials while ensuring spatial and temporal control as well as safety. Therefore, a novel osteoinductive nonviral in vivo gene therapy approach using sonoporation was investigated in ectopic and orthotopic models. Constitutive or regulated, doxycycline-inducible, bone morphogenetic protein 2 and 7 coexpression plasmids were repeatedly applied for 5 days. Ectopic and orthotopic gene transfer efficacy was monitored by coapplication of a luciferase plasmid and bioluminescence imaging. Orthotopic plasmid DNA distribution was investigated using a novel plasmid-labeling method. Luciferase imaging demonstrated an increased trend (61% vs. 100%) of gene transfer efficacy, and micro-computed tomography evaluation showed significantly enhanced frequency of ectopic bone formation for sonoporation compared with passive gene delivery (46% vs. 100%) dependent on applied ultrasound power. Bone formation by the inducible system (83%) was stringently controlled by doxycycline in vivo, and no ectopic bone formation was observed without induction or with passive gene transfer without sonoporation. Orthotopic evaluation in a rat femur segmental defect model demonstrated an increased trend of gene transfer efficacy using sonoporation. Investigation of DNA distribution demonstrated extensive binding of plasmid DNA to bone tissue. Sonoporated animals displayed a potentially increased union rate (33%) without extensive callus formation or heterotopic ossification. We conclude that sonoporation of BMP2/7 coexpression plasmids is a feasible, minimally invasive method for osteoinduction and that improvement of bone regeneration by sonoporative gene delivery is superior to passive gene delivery.

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Section: Orthotopic Bmp2/7 Sonoporationmentioning

confidence: 82%

Sonoporation Increases Therapeutic Efficacy of Inducible and Constitutive BMP2/7 In Vivo Gene Delivery

Feichtinger

Hofmann

Slezak

et al. 2014

Human Gene Therapy Methods

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“…Recent investigations have shown that the combined effect of magnetic and hydroxyapatite nanostructures provides high drug loading capacity and an efficient drug delivery to specific targets with a controlled external magnetic field. Especially, the HAp/Fe 3 O 4 nanocomposites are promising bio-nanomaterials for various applications like targeted drug delivery, orthopaedic, protein adsorption/pH dependent controlled release, hyperthermia-based anticancer treatments, and solid phase extraction of plasmid DNA [8,13,14,15]. Therefore, the synthesis of HAp/Fe 3 O 4 nanocomposites is in high demand for biomedical drug delivery applications.…”

Section: Introductionmentioning

confidence: 99%

Designed Synthesis of Nanostructured Magnetic Hydroxyapatite Based Drug Nanocarrier for Anti-Cancer Drug Delivery toward the Treatment of Human Epidermoid Carcinoma

et al. 2017

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Superparamagnetic Fe3O4 nanoparticles on hydroxyapatite nanorod based nanostructures (Fe3O4/HAp) were synthesized using hydrothermal techniques at 180 °C for 12 h and were used as drug delivery nanocarriers for cancer cell therapeutic applications. The synthesized Fe3O4/HAp nanocomposites were characterized by X-ray diffraction analysis (XRD), Field emission scanning electron microscopy (FESEM), Fourier transform infrared spectroscopy (FTIR), Brunauer-Emmett-Teller (BET)-analysis, and vibrating sample magnetometry (VSM). The morphologies of the Fe3O4/HAp nanocomposites show 15 nm Fe3O4 nanoparticles dispersed in the form of rods. The BET result shows that the synthesized samples have a high specific surface area of 80 m2 g−1 with mesoporous structures. Magnetic measurements revealed that the sample has high saturation magnetization of 18 emu/g with low coercivity. The Fe3O4/HAp nanocomposites had a large specific surface area (SSA), high mesoporous volume, and good magnetic property, which made it a suitable nanocarrier for targeted drug delivery systems. The chemotherapeutic agent, andrographolide, was used to investigate the drug delivery behavior of the Fe3O4/HAp nanocomposites. The human epidermoid skin cancer cells (A431) were used as the model targeting cell lines by treating with andrographolide loaded Fe3O4/HAp nanosystems and were further evaluated for their antiproliferative activities and the induction of apoptosis. Also, the present nanocomposite shows better biocompatibility, therefore it can be used as suitable drug vehicle for cancer therapy applications.

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“…Calcium hydroxide [Ca(OH) 2 ] was reacted with 65 Shan et al reported a similar wet precipitation method for synthesizing MHAp for solidphase plasmid DNA (pDNA) extraction. 66 Zuo et al synthesized laminated MHAp via a solution intercalation method. In their study, 1.0 g sodium dodecyl sulfonate was dissolved in 15 mL deionized (DI) water with 30 mL ethanol, followed by heating at 60°C.…”

Section: Different Synthesis Routes Of Magnetic Hapmentioning

confidence: 99%

“…This method of MHAp synthesis is simple, fast, and cost-effective without the requirement of further purification via centrifugation and hazardous chemical solvents. 66 Zuo et al synthesized laminated MHAp/DNA nanohybrid using a solution intercalation method. The prepared laminated MHAp exhibited an effective DNA-protecting property.…”

mentioning

confidence: 99%

Magnetic hydroxyapatite: a promising multifunctional platform for nanomedicine application

Mondal¹,

Manivasagan²,

Bharathiraja³

et al. 2017

IJN

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In this review, specific attention is paid to the development of nanostructured magnetic hydroxyapatite (MHAp) and its potential application in controlled drug/gene delivery, tissue engineering, magnetic hyperthermia treatment, and the development of contrast agents for magnetic resonance imaging. Both magnetite and hydroxyapatite materials have excellent prospects in nanomedicine with multifunctional therapeutic approaches. To date, many research articles have focused on biomedical applications of nanomaterials because of which it is very difficult to focus on any particular type of nanomaterial. This study is possibly the first effort to emphasize on the comprehensive assessment of MHAp nanostructures for biomedical applications supported with very recent experimental studies. From basic concepts to the real-life applications, the relevant characteristics of magnetic biomaterials are patented which are briefly discussed. The potential therapeutic and diagnostic ability of MHAp-nanostructured materials make them an ideal platform for future nanomedicine. We hope that this advanced review will provide a better understanding of MHAp and its important features to utilize it as a promising material for multifunctional biomedical applications.

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Application of magnetic hydroxyapatite nanoparticles for solid phase extraction of plasmid DNA

Cited by 31 publications

References 12 publications

Sonoporation Increases Therapeutic Efficacy of Inducible and Constitutive BMP2/7 In Vivo Gene Delivery

Sonoporation Increases Therapeutic Efficacy of Inducible and Constitutive BMP2/7 In Vivo Gene Delivery

Designed Synthesis of Nanostructured Magnetic Hydroxyapatite Based Drug Nanocarrier for Anti-Cancer Drug Delivery toward the Treatment of Human Epidermoid Carcinoma

Magnetic hydroxyapatite: a promising multifunctional platform for nanomedicine application

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