Application of magnetic immobilized enzyme of nano dialdehyde starch in deacidification of rice bran oil

Facile Multilayer Deposition of Polyethylenimine and κ-Carrageenan on a 3D-Printed Lattice for Cellulase Immobilization

“…The self-oligomerization of GA generates longer GA chains, leading to the formation of multipoint covalent interaction that can cause the self-interaction between adjacent GA chains, resulting in the loss of potential active sites for enzyme binding. − Moreover, the excessive presence of free self-oligomerized longer GA chains atop the lattice surface would also result in the deactivation of the enzyme active site. The deactivation could be attributed to the formation of sturdy pyridine rings on the enzyme active site, subsequently altering the enzyme conformation . As a result of the aldol condensations of GA that involve both Schiff-base formation to produce cyclic hemiacetal and Michael-type 1,4-addition to α,β-unsaturated aldehyde moieties, the longer self-oligomerized GA chains form multipoint covalent interaction with the amino groups on the enzyme, forming cyclic complexes. , This explained the decline of the relative activity as a high GA concentration was employed.…”

“…The deactivation could be attributed to the formation of sturdy pyridine rings on the enzyme active site, subsequently altering the enzyme conformation. 37 As a result of the aldol condensations of GA that involve both Schiff-base formation to produce cyclic hemiacetal and Michael-type 1,4-addition to α,β-unsaturated aldehyde moieties, the longer self-oligomerized GA chains form multipoint covalent interaction with the amino groups on the enzyme, forming cyclic complexes. 38,39 This explained the decline of the relative activity as a high GA concentration was employed.…”

Enzymes Immobilized into Starch- and Gelatin-Based Hydrogels: Properties and Application in Inhibition Assay

“…Dialdehyde starch was used as a coupling agent to prepare a chitosan carrier to immobilize the xylanase from Aspergillus niger [62]. Also, dialdehyde starch with nanocrystallization was used as a cross-linking agent to immobilize Candida Antarctica lipase B on a carrier with Fe 3 O 4 as the core [63]. The same is true of gelatin.…”

Recent Advances in Enzyme Immobilisation Strategies: An Overview of Techniques and Composite Carriers

“…The matrices or supports for this novel technology can be categorised into the following: organic and inorganic compounds. The most reported enzyme supports of organic materials are organic membrane [76], chitosan [77,78], alginate [54], resin [79], collagen [80], gelatine [81], dextran [82], starch [83], carrageenan [42], agarose [84], protein [85], cellulose [86], activated carbon [87], agar [88], and chitin [89]. Synthetic organic materials are polyvinyl alcohol [90], polyurethane foam [91], polyacrylonitrile [92], polyethylene, polypropylene membrane, and polyacrylamide [93][94][95].…”