Application of Magnetic Levitation Induced Weightlessness to Detect Cell Lineage

Abstract

Identification and classification of bone marrow cells is an important step for molecular biology and therapeutic studies related to bone marrow disorders such as osteoporosis or obesity. In this study, we applied magnetic levitation technology to induce a weightlessness environment to detect adipocytes and osteoblasts based on their single cell density. This biotechnological method can be used for separation of heterogeneous populations such as bone marrow once adapted to a continuous microfluidic platform.

Scaffold‐free biofabrication of adipocyte structures with magnetic levitation

“…In recent studies, the label‐free magnetic levitation technique has been used for detection and characterization of various cell types such as blood cells (Durmus et al, 2015; Ge et al, 2018; Savas Tasoglu et al, 2015; Yenilmez et al, 2016), cancer cells (Durmus et al, 2015; Stephanie Knowlton et al, 2017), adipocytes (Sarigil, Anil‐Inevi, Yilmaz, Mese, et al, 2019), and osteogenic cells (Sarigil, Anil‐Inevi, Yilmaz, Cagan, et al, 2019). Also, it was shown that sickle cell disease which results in increased density of erythrocytes (Knowlton et al, 2015) and drug treatments that cause density changes in bacteria and yeast (Durmus et al, 2015) can be tested with magnetic levitation systems and portable platforms of the system enable applications for point of care testing (Stephanie Knowlton et al, 2017; Yenilmez, Knowlton, & Tasoglu, 2016; Yenilmez, Knowlton, Yu, et al, 2016).…”

Magnetic levitation assisted biofabrication, culture, and manipulation of 3D cellular structures using a ring magnet based setup

“…Cells, that exhibit greater magnetic susceptibility than their surrounding buffer or medium due to labeling with magnetic particles or a rare intrinsic property of some cell types (i.e., paramagnetic hemoglobin containing red blood cells and magnetotactic bacteria), move towards regions of the high magnetic field (positive magnetophoresis) (Pamme,2006). However, most cell types are diamagnetic in nature, and once placed into a surrounding environment with high magnetic susceptibility, they are repelled towards the minimal magnetic field (negative magnetophoresis, also referred to as diamagnetophoresis) (Anil‐Inevi et al,2019b; Durmus et al,2015; Sarigil et al,2019a,2019b; Winkleman et al,2004). Stable cell trapping and self‐assembly have been previously conducted by both positive and negative magnetophoresis to create viable 3D structures (Anil‐Inevi et al,2018; Haisler et al,2013; Sarigil et al,2020).…”

“…However, most cell types are diamagnetic in nature, and once placed into a surrounding environment with high magnetic susceptibility, they are repelled towards the minimal magnetic field (negative magnetophoresis, also referred to as diamagnetophoresis) (Anil-Inevi et al, 2019b;Durmus et al, 2015;Sarigil et al, 2019aSarigil et al, , 2019bWinkleman et al, 2004). Stable cell trapping and self-assembly have been previously conducted by both positive and negative magnetophoresis to create viable 3D structures (Anil-Inevi et al, 2018;Haisler et al, 2013;Sarigil et al, 2020).…”

Axial-circular magnetic levitation assisted biofabrication and manipulation of cellular structures

“…paramagnetic hemoglobin containing blood cells and magnetotactic bacteria), move towards regions of the high magnetic field (positive magnetophoresis) [11]. However, most cell types are diamagnetic in nature, and once placed into a surrounding environment with high magnetic susceptibility, they are repelled towards the minimal magnetic field (negative magnetophoresis, also referred to as diamagnetophoresis) [12][13][14][15][16][17]. Stable cell trapping and selfassembly have been previously conducted by both positive and negative magnetophoresis to create viable 3D structures [18][19][20].…”