Application of MALDI-TOF MS for requalification of a Candida clinical isolates culture collection

Lima-Neto, Reginaldo Gonçalves de; Santos, Cledir; Lima, Nelson; Sampaio, Paula; Pais, Célia; Neves, Rejane Pereira

doi:10.1590/s1517-83822014005000044

Cited by 47 publications

(37 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Its remarkable reproducibility is based on measurement of proteins in a mass range between 2000 and 20,000 Da, where important ribosomal proteins could be used as biomarkers. The entire process is reviewed and described in detail for analysis of yeasts [19,22] and filamentous fungi [20,23]. At present, several important contributions have been published on MALDI-TOF MS for fungal identification [24e31].…”

Section: Introductionmentioning

confidence: 99%

Development and optimization of a new MALDI-TOF protocol for identification of the Sporothrix species complex

Oliveira

Santos

Sampaio

et al. 2015

Research in Microbiology

Self Cite

View full text Add to dashboard Cite

Accurate species identification of the Sporothrix schenckii complex is essential, since identification based only on phenotypic characteristics is often inconclusive due to phenotypic variability within the species. We used matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) for species identification of 70 environmental and clinical isolates of the Sporothrix complex. A reference database was established for MALDI-TOF MS-based species identification according to minor adjustments in the manufacturer's guidelines. The MALDI-TOF MS clearly distinguished strains of Sporothrix brasiliensis, Sporothrix globosa, Sporothrix mexicana, S. schenckii, Sporothrix luriei and Sporothrix pallida, enabling identification of all isolates at the species level, as confirmed by partial calmodulin gene sequence analyses. The present methodology is simple, reliable, rapid and highly suitable for routine identification in clinical mycology laboratories and culture collections, particularly for updating and reclassifying of deposited Sporothrix isolates.

show abstract

Section: Introductionmentioning

confidence: 99%

Development and optimization of a new MALDI-TOF protocol for identification of the Sporothrix species complex

Oliveira

Santos

Sampaio

et al. 2015

Research in Microbiology

Self Cite

View full text Add to dashboard Cite

show abstract

“…Homogenous inoculum of yeast cells were grown and maintained on Yeast Extract Peptone Dextrose Agar medium (YEPD). Incubations were performed at 20h and strains were grown aerobically at 37°C according to Lima-Neto et al [ 25 ]. In order to avoid changes in the protein expression pattern, the culture conditions and growth time were standardized as described above.…”

Section: Methodsmentioning

confidence: 99%

A new approach by optical coherence tomography for elucidating biofilm formation by emergent Candida species

et al. 2017

View full text Add to dashboard Cite

The majority of microorganisms present a community lifestyle, establishing biofilm ecosystems. However, little is known about its formation in emergent Candida species involved in catheter-related infections. Thus, various techniques may be used in the biofilm detection to elucidate structure and clinical impact. In this context, we report the ability of emergent Candida species (Candida haemulonii, C. lusitaniae, C. pelliculosa, C.guilliermondii, C. famata and C. ciferrii) on developing well structured biofilms with cell viability and architecture, using optical coherence tomography (OCT). This new approach was compared with XTT analyses and Scanning Electron Microscopy (SEM). A positive correlation between oxidative activity (XTT) and OCT results (r = 0.8752, p < 0.0001) was observed. SEM images demonstrated cells attachment, multilayer and morphologic characteristics of the biofilm structure. C. lusitaniae was the emergent species which revealed the highest scattering extension length and oxidative metabolism when evaluated by OCT and XTT methods, respectively. Herein, information on C. ciferri biofilm structure were presented for the first time. The OCT results are independently among Candida strains and no species-specific pattern was observed. Our findings strongly contribute for clinical management based on the knowledge of pathogenicity mechanisms involving emergent yeasts.

show abstract

“…Mass spectrometry, based on the matrix-assisted laser desorption ionization time-of-flight (MALDI TOF) methodology, has emerged as a valuable method for the identification of microorganisms, and with good performance in the identification of yeasts, for its speed and precision 5 , 6 . For these reasons, this technology has been used more frequently in clinical laboratories, the commercial systems Microflex ® (Bruker Daltonics GmbH, Leipzig, Germany) and Vitek ® MS (bioMérieux, Marcy, L'Etoile, France) being the most popular 6 , 7 .…”

Section: Introductionmentioning

confidence: 99%

Concordance analysis between different methodologies used for identification of oral isolates of Candida species

et al. 2018

View full text Add to dashboard Cite

Background: The yeasts species determination is fundamental not only for an accurate diagnosis but also for establishing a suitable patient treatment. We performed a concordance study of five methodologies for the species identification of oral isolates of Candida in Colombia. Methods: Sixty-seven Candida isolates were tested by; API® 20C-AUX, Vitek®2 Compact, Vitek®MS, Microflex® and a molecular test (panfungal PCR and sequencing). The commercial cost and processing time of the samples was done by graphical analysis. Results: Panfungal PCR differentiated 12 species of Candida, Vitek®MS and Microflex® methods identified 9 species, and API® 20C-AUX and Vitek®2 Compact methods identified 8 species each. Weighted Kappa (wK) showed a high agreement between Panfungal PCR, Vitek®MS, Microflex® and API® 20C-AUX (wK 0.62-0.93). The wK that involved the Vitek®2 Compact method presented moderate or good concordances compared with the other methods (wK 0.56-0.73). Methodologies based on MALDI TOF MS required 4 minutes to generate results and the Microflex® method had the lowest selling price. Conclusion: The methods evaluated showed high concordance in their results, being higher for the molecular methods and the methodologies based on MALDI TOF. The latter are faster and cheaper, presenting as promising alternatives for the routine identification of yeast species of the genus Candida.

show abstract

Application of MALDI-TOF MS for requalification of a Candida clinical isolates culture collection

Cited by 47 publications

References 28 publications

Development and optimization of a new MALDI-TOF protocol for identification of the Sporothrix species complex

Development and optimization of a new MALDI-TOF protocol for identification of the Sporothrix species complex

A new approach by optical coherence tomography for elucidating biofilm formation by emergent Candida species

Concordance analysis between different methodologies used for identification of oral isolates of Candida species

Contact Info

Product

Resources

About