Application of Mercury Cold Vapor Atomic Fluorescence Spectrometry to the Characterization of Mercury-Accessible −SH Groups in Native Proteins

Bramanti, Emilia; D’Ulivo, Alessandro; Lampugnani, Leonardo; Zamboni, R.; Raspi, Giorgio

doi:10.1006/abio.1999.4257

Cited by 28 publications

(40 citation statements)

References 30 publications

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“…In the selected conditions, 511 2 of 4 -SH groups of OVA are very reactive and immediately bind 512 pHMB. The complexation of the third group is much slower, requir-513ing up to 24 h, whereas the fourth group is not significantly com-514 plexed even after 24 h. This is in agreement with the results we 515 had previously found[35] in the titration of OVA-Hg(II) complexes 516 with NaBH 4 . The reduction curve of OVA-Hg(II) with NaBH 4 had,517 indeed, two inflection points.…”

supporting

confidence: 93%

“…The protein was pre-column 32 simultaneously denatured and derivatized, and the pHMB-labeled denatured OVA complexes were ana-33 lyzed by size exclusion chromatography (SEC) coupled online with chemical vapor generation-atomic 34 fluorescence spectrometry (CVG-AFS). The number of -SH groups titrated greatly depends on the protein 35 structure in solution. Indeed, we found that, depending on the adopted denaturing conditions, OVA gave 36 different aggregate species that influence the complexation process.…”

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confidence: 99%

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Ovalbumin labeling with p-hydroxymercurybenzoate: The effect of different denaturing agents and the kinetics of reaction

Campanella

Onor

Biancalana

et al. 2015

Analytical Biochemistry

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confidence: 93%

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confidence: 99%

Ovalbumin labeling with p-hydroxymercurybenzoate: The effect of different denaturing agents and the kinetics of reaction

Campanella

Onor

Biancalana

et al. 2015

Analytical Biochemistry

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“…Because Pb binds exclusively to hemoglobin molecule in erythrocytes [59], a VLBW infant who might have been transfused with pRBC would receive nearly double the concentration of heavy metals initially present in the donor blood [15]. Several studies suggest that other metals also bind to hemoglobin [60][61][62][63][64][65]. The difference in metal concentrations between whole blood versus pRBCs may not be a trivial issue.…”

Section: Discussionmentioning

confidence: 99%

Toxic Metal Contamination of Banked Blood Designated for Neonatal Transfusion

Sundararajan¹,

Blatz²,

Dearborn³

et al. 2015

J Clin Toxicol

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Objective: Very low birth weight (VLBW) infants frequently receive blood transfusions. We hypothesize that toxic metals in donor blood may pose a health risk with potential adverse neurologic effects on the developing brain of a vulnerable VLBW infant. Study design:Samples from 100 donor blood units were collected from a large urban hospital. Blood was analyzed for aluminum, arsenic, beryllium, cadmium, manganese, mercury, nickel, lead and polonium. The estimated upper limit of acceptable metal concentration in donor blood was calculated assuming a transfusion volume of 20 ml/kg and using either previously published acceptable intravenous doses or oral reference doses with a conservative estimate of 10% gastrointestinal absorption. Ingested mercury was assumed to be 95% absorbed.Results: Eight of the nine metals were detectable. Concentrations of arsenic, beryllium, cadmium, mercury and polonium were not of concern for any single blood transfusion. Concentrations of aluminum, manganese, nickel and lead exceeded the estimated upper limit of acceptable concentration in 5, 11, 4 and 26 units respectively. Of the 100 units, 31 had at least one toxic metal concentration high enough to pose a potential health risk.Conclusions: VLBW infants are exposed to heavy metals that are toxic from blood transfusion. The number of units with concerning levels of toxic metals was higher than expected. Neonatologists should be aware of this potential exposure to toxic metals from donor blood when decision is made to administer blood transfusion. Neurodevelopmental studies of toxic metal exposed infants from blood transfusion are warranted.

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“…The enzymatic activity on the volume fraction curresponding to the elution profile of the enzyme was also assessed. Alternatively the number of -SH groups that can react with inorganic or organic mercury (PHMB) can be analysed by cold-vapor atomic fluorescence spectrometry (CVAFS) [21] or HIC-CVAFS hyphenated apparatus [7]. This alternative approach is appropriate as all the protein reported are thiolic proteins.…”

Section: Fraction D (Determination Of Pgi and Pgm As Phm B-enzymes Comentioning

confidence: 99%

Simultaneous determinations of glycolytic enzymes in crude extract concentrate by hydrophobic interaction chromatography

et al. 2001

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SummaryPreviously described methods for the simultaneous determination of some glycolytic enzymes by hydrophobic interaction chromatography (HIC) have been further developed in order to identify and quantitate similar enzymes in extract concentrates. Previously reported problems arising from difficult elution, broad peaks production and coelution have been overcome by collecting separate fractions of HIC eluate of the original sample. These fractions, which contain selected mixtures of troubling enzymes, are reinjected using an appropriate choice of operating conditions involving hydrophobic phase, sample treatment and elution conditions. Using slight changes to the previous procedures, mixtures of myokinase/triosephosphate isomerase, glyceraldehyde 3-phosphate dehydrogenase/3-phosphoglyceric phosphokinase, pyruvate kinase/blactate dehydrogenase/creatine phosphokinase and phosphoglucose isomerase/phosphoglucose mutase can be resolved. This allows easy identification and simultaneous determination of related enzymes. Results obtained in the application to commercial crudes produced near quantitative recoveries of examined enzymes. Determination of these compounds was found to be precise with a mean coefficient of variation ranging from 2.6% to 4.2%.

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Application of Mercury Cold Vapor Atomic Fluorescence Spectrometry to the Characterization of Mercury-Accessible −SH Groups in Native Proteins

Cited by 28 publications

References 30 publications

Ovalbumin labeling with p-hydroxymercurybenzoate: The effect of different denaturing agents and the kinetics of reaction

Ovalbumin labeling with p-hydroxymercurybenzoate: The effect of different denaturing agents and the kinetics of reaction

Toxic Metal Contamination of Banked Blood Designated for Neonatal Transfusion

Simultaneous determinations of glycolytic enzymes in crude extract concentrate by hydrophobic interaction chromatography

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