Application of mesenchymal stem cells derived from human pluripotent stem cells in regenerative medicine

Abstract

Mesenchymal stem cells (MSCs) represent the most clinically used stem cells in regenerative medicine. However, due to the disadvantages with primary MSCs, such as limited cell proliferative capacity and rarity in the tissues leading to limited MSCs, gradual loss of differentiation during in vitro expansion reducing the efficacy of MSC application, and variation among donors increasing the uncertainty of MSC efficacy, the clinical application of MSCs has been greatly hampered. MSCs derived from human pluripotent stem cells (hPSC-MSCs) can circumvent these problems associated with primary MSCs. Due to the infinite self-renewal of hPSCs and their differentiation potential towards MSCs, hPSC-MSCs are emerging as an attractive alternative for regenerative medicine. This review summarizes the progress on derivation of MSCs from human pluripotent stem cells, disease modelling and drug screening using hPSC-MSCs, and various applications of hPSC-MSCs in regenerative medicine. In the end, the challenges and concerns with hPSC-MSC applications are also discussed.

Role and Function of Mesenchymal Stem Cells on Fibroblast in Cutaneous Wound Healing

“…Many studies have been conducted to resolve these problems, to recognize proteins expressed on the cell surface of MSCs as antigenic markers, and to isolate high-quality MSCs by analyzing their expression patterns using several types of markers [ 63 ]. Therefore, the collection of high-purity, higher-quality MSCs and the management of cultured MSCs for their use in regenerative medicine is a problem that needs to be solved as soon as possible [ 64 , 65 ]. Although MSCs can be used for many diseases and have significant advantages, there still are issues in establishing collection methods in clinical settings.…”

Research progress of stem cell therapy for endometrial injury

“…So far, stem cell therapy has become a new hope for the treatment of many incurable diseases, but still faces problems such as low engraftment rate, limited efficacy, tumor formation, storage, and transportation [ [184] , [185] , [186] , [187] ]. Moreover, the sources of stem cells are very limited, and the acquisition process often causes great damage or adverse effects on the psychological and physiological conditions of recipients and donors [ 188 ].…”

Mesenchymal stem/stromal cells in the pathogenesis and regenerative therapy of inflammatory bowel diseases

“…MSCs can differentiate from other stem cells. Human pluripotent stem cells (hPSCs) include human embryonic stem cells (hESCs) and induced pluripotent stem cells (iPSCs), both of which can differentiate into MSCs ( 133 ). MSCs derived from hESCs and iPSCs exhibited similar properties, such as their ability to secrete anti-inflammatory soluble factors, and to restore the intestinal mucosal barrier in IBDs ( 134 ).…”