Application of metagenomic next-generation sequencing in cutaneous tuberculosis

Kang, Min; Li, Wei; Kong, Qingsheng; Hua, Dong; Han, Ahram; Jiang, Li‐Qing

doi:10.3389/fcimb.2022.942073

Cited by 11 publications

(7 citation statements)

References 48 publications

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“… 1 Of note, with the widespread of next generation sequencing technology, mNGS was applied into the differential diagnosis of cutaneous infection. 8 Zhang et al managed to distinguished a pyoderma gangrenosum-like ulcer caused by Helicobacter cinaedi using mNGS. 9 In this case, the combination of negative results of serological, cultured test and mNGS assist us to exclude a skin infection and further make a diagnosis of PG.…”

Section: Discussionmentioning

confidence: 99%

Progressive Facial Ulcer: A Case Report of Pyoderma gangrenosum

Zhang,

Tang,

Jing

2024

JIR

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Pyoderma gangrenosum (PG) is a rare neutrophilic dermatosis characterized by rapidly developing and painful skin ulcers with distinctive features. As far as we are concerned, there is no previous case report on facial PG in East-Asia. In this case, we describe a case of a 79-year-old man with a 3-month history of progressive painful ulcers on his cheek and upper lip. Initial suspicion of atypical mycobacterium infection led to an ineffective treatment regimen. Comprehensive infectious testing yielded negative results, and a positive pathergy test indicated a potential diagnosis of PG. A skin biopsy confirmed the diagnosis, and the patient showed significant improvement with intravenous methylprednisolone and oral cyclosporine treatment. After three months, complete resolution of the lesions was achieved without recurrence. The case highlights the diagnostic challenges associated with PG, which is often misdiagnosed due to its resemblance to other conditions. Thorough evaluation is crucial to exclude alternative diagnoses, particularly cutaneous infections. Clinical morphology, tissue biopsy, and culture are essential for accurate diagnosis. The presence of pathergy, the development of new lesions following minor trauma, can also be a diagnostic clue.

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Section: Discussionmentioning

confidence: 99%

Progressive Facial Ulcer: A Case Report of Pyoderma gangrenosum

Zhang,

Tang,

Jing

2024

JIR

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“…The application of NGS in recognizing clinical pathogens remains in the primary phase, and no mNGS procedures are approved by the Food and Drug Management Administration ( Kim et al, 2016 ). Consequently, the current NGS procedures are personalized, and there is a long way to go to accomplish standardized detection ( Kong et al, 2022 ). Moreover, the restricted capacity of NGS to detect low-frequency variations is one of its major drawbacks ( Cohen et al, 2021 ).…”

Section: Conclusion and Prospectsmentioning

confidence: 99%

Application of next-generation sequencing to identify different pathogens

Nafea,

Wang,

Wang

et al. 2024

Front. Microbiol.

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Early and precise detection and identification of various pathogens are essential for epidemiological monitoring, disease management, and reducing the prevalence of clinical infectious diseases. Traditional pathogen detection techniques, which include mass spectrometry, biochemical tests, molecular testing, and culture-based methods, are limited in application and are time-consuming. Next generation sequencing (NGS) has emerged as an essential technology for identifying pathogens. NGS is a cutting-edge sequencing method with high throughput that can create massive volumes of sequences with a broad application prospects in the field of pathogen identification and diagnosis. In this review, we introduce NGS technology in detail, summarizes the application of NGS in that identification of different pathogens, including bacteria, fungi, and viruses, and analyze the challenges and outlook for using NGS to identify clinical pathogens. Thus, this work provides a theoretical basis for NGS studies and provides evidence to support the application of NGS in distinguishing various clinical pathogens.

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“…In that way, it becomes possible to identify not only the classical pathogens, but also emerging pathogens or difficult-to-growth bacteria like anaerobes. Thus, NGS-based approaches could provide pivotal and unbiased information for the stewardship of antimicrobial therapy in SSTI, especially in case of polymicrobial or fungal infection and in the immunocompromised host [80][81][82]. This possibility should already be considered in these contexts as a second line option when classical methods have failed.…”

Section: Concluding Remarks and Perspectivesmentioning

confidence: 99%

Rapid diagnostics for skin and soft tissue infections: the current landscape and future potential

Barbier

Woerther

Timsit

2023

Current Opinion in Infectious Diseases

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Purpose of reviewManaging antimicrobial therapy in patients with complicated skin and soft tissue infections (SSTI) constitutes a growing challenge due to the wide spectrum of potential pathogens and resistance phenotypes. Today, microbiological documentation relies on cultural methods. This review summarizes the available evidence regarding the clinical input of rapid microbiological diagnostic tools (RMDT) and their impact on the management of antimicrobial therapy in SSTI.Recent findingsAccurate tools are already available for the early detection of methicillin-resistant Staphylococcus aureus (MRSA) in SSTI samples and may help avoiding or shortening empirical anti-MRSA coverage. Further research is necessary to develop and evaluate RMDT detecting group A streptococci (e.g., antigenic test) and Gram-negative pathogens (e.g., multiplex PCR assays), including through point-of-care utilization. Next-generation sequencing (NGS) methods could provide pivotal information for the stewardship of antimicrobial therapy, especially in case of polymicrobial or fungal SSTI and in the immunocompromised host; however, a shortening in the turnaround time and prospective data regarding their therapeutic input are needed to better appraise the clinical positioning of these promising approaches.SummaryThe clinical input of RMDT in SSTI is currently limited due to the scarcity of available dedicated assays and the polymicrobial feature of certain cases. NGS appears as a relevant tool but requires further developments before its implementation in routine clinical practice.

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Application of metagenomic next-generation sequencing in cutaneous tuberculosis

Cited by 11 publications

References 48 publications

Progressive Facial Ulcer: A Case Report of Pyoderma gangrenosum

Progressive Facial Ulcer: A Case Report of Pyoderma gangrenosum

Application of next-generation sequencing to identify different pathogens

Rapid diagnostics for skin and soft tissue infections: the current landscape and future potential

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