Application of micro<scp>RNA</scp> gene resources in the improvement of agronomic traits in rice

Zheng, Ling‐Ling; Qu, Liang‐Hu

doi:10.1111/pbi.12321

Cited by 50 publications

(32 citation statements)

References 95 publications

(110 reference statements)

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“…Finally, 208 miRNAs including 148 known and 60 novel miRNAs in tall fescue were obtained by blastx searching against mature miRNAs from Brachypodium distachyon, Oryza sativa, Zea mays, Hordeum vulgare, Aegilops tauschii and Festuca arundinacea, which were more than the tillering-responsive small RNAs (32 miRNAs) identi ed in switchgrass through a genome-wide analysis [29]. In this study, the length of the miRNAs ranged from 18 to 24 nt, which was consistent with the previous reports [25,30]. In wheat, 420 miRNAs were obtained to analyze the regulation networks of tillering [28].…”

Section: Discussionsupporting

confidence: 88%

Genome-wide small RNA profiling reveals tiller development in tall fescue (Festuca arundinacea Schreb.)

Wang

et al. 2020

Preprint

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Background: Tall fescue (Festuca arundinacea Schreb.) is a major cool-season forage and turfgrass species. The low tiller density and size dramatically limits its turf performance and forage yield. MicroRNAs (miRNA)-genes modules play critical roles in tiller development in plants. In this study, a genome-wide small RNA profiling was carried out in two tall fescue genotypes contrasting for tillering production (‘Ch-3’, high tiller production rate and ‘Ch-5’, low tiller production rate) and two types of tissue samples at different tillering development stage (Pre-tillering, grass before tillering; Tillering, grass after tillering). ‘Ch-3’, ‘Ch-5’, Pre-tillering, and Tillering samples were analyzed using high-throughput RNA sequencing. Results: A total of 222 million high-quality clean reads were generated and 208 miRNAs were discovered, including 148 known miRNAs belonging to 70 families and 60 novel ones. Furthermore, 18 miRNAs were involved in tall fescue tiller development process. Among them, 14 miRNAs displayed increased abundance in both Ch-3 and Tillering plants compared with that in Ch-5 and Pre-tillering plants and were positive with tillering, while another four miRNAs were negative with tiller development. Out of the three miRNAs osa-miR156a, zma-miR528a-3p and osa-miR444b.2, the rest of 15 miRNAs were newfound and associated with tiller development in plants. Based on our previous full-length transcriptome analysis in tall fescue, 2 8927 potential target genes were discovered for all identified miRNAs. Most of the 212 target genes of the 18 miRNAs were dominantly enriched into “ubiquitin-mediated proteolysis”, “phagosome”, “fatty acid biosynthesis”, “oxidative phosphorylation”, and “biosynthesis of unsaturated fatty acids” KEGG pathways. In addition, bdi-miR167e-3p targets two kinase proteins EIF2AK4 and IRAK4, and osa-miR397a targets auxin response factor 5, which may be the significant miRNA-genes controllers in tillering development. Conclusions: This is the first genome-wide miRNA profiles analysis to identify regulators involved in tiller development in cool-season turfgrass. Tillering related 18 miRNAs and their 212 target genes provide novel information for the understanding of the molecular mechanisms of miRNA-genes mediated tiller development in cool-season turfgrass.

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Section: Discussionsupporting

confidence: 88%

Genome-wide small RNA profiling reveals tiller development in tall fescue (Festuca arundinacea Schreb.)

Wang

et al. 2020

Preprint

View full text Add to dashboard Cite

show abstract

“…Finally, 208 miRNAs including 148 known and 60 novel miRNAs in tall fescue were obtained by blastx searching against mature miRNAs from Brachypodium distachyon, Oryza sativa, Zea mays, Hordeum vulgare, Aegilops tauschii and Festuca arundinacea, which were more than the tilleringresponsive small RNAs (32 miRNAs) identi ed in switchgrass through a genome-wide analysis [28]. In this study, the length of the miRNAs ranged from 18 to 24 nt, which was consistent with the previous reports [24,29]. In wheat, 420 miRNAs were obtained to analyze the regulation networks of tillering [27].…”

Section: Discussionsupporting

confidence: 82%

Genome-wide Small Rna Profiling Reveals Tiller Development in Tall Fescue (Festuca Arundinacea Schreb.)

Wang

et al. 2020

Preprint

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Background: Tall fescue (Festuca arundinacea Schreb.) is a major cool-season forage and turfgrass species. The low tiller density and size dramatically limits its turf performance and forage yield. MicroRNAs (miRNA)-genes modules play critical roles in tiller development in plants. In this study, a genome-wide small RNA profiling was carried out in two tall fescue genotypes contrasting for tillering production (‘Ch-3’, high tiller production rate and ‘Ch-5’, low tiller production rate) andtwo types of tissue samples at different tillering development stage (Pre-tillering, grass before tillering; Tillering, grass after tillering). ‘Ch-3’, ‘Ch-5’, Pre-tillering, and Tillering samples were analyzed using high-throughput RNA sequencing.Results: A total of 222 million high-quality clean reads were generated and 208 miRNAs were discovered, including 148 known miRNAs belonging to 70 families and 60 novel ones. Furthermore, 18 miRNAs were involved in tall fescue tiller development process. Among them, 14 miRNAs displayed increased abundance in both Ch-3 and Tillering plants compared with that in Ch-5 and Pre-tillering plants and were positive with tillering, while another four miRNAs were negative with tiller development. Out ofthe three miRNAs osa-miR156a, zma-miR528a-3p and osa-miR444b.2, the rest of 15 miRNAs were newfound controllers mediating tiller development in plants. Based on our previous full-length transcriptome analysis in tall fescue, 2 8927 potential target genes were discoveredfor all identified miRNAs. Most of the 212 target genes of the 18 miRNAs were dominantly enriched into “ubiquitin-mediated proteolysis”, “phagosome”, “fatty acid biosynthesis”, “oxidative phosphorylation”, and “biosynthesis of unsaturated fatty acids” KEGG pathways.Conclusions: This is the first genome-wide miRNA profiles analysis to identifyregulators involved in tiller development in cool-season turfgrass. Tillering related 18 miRNAs and their 212 target genes provide novel information for the understanding of the molecular mechanisms of miRNA-genes mediated tiller development in cool-season turfgrass.

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“…Schools of science education in many countries are often a race in order to pass exams to the next school year and ultimately universities (Paton, 2012;Zheng & Qu, 2015). Therefore, the instruction may be focused on the topics that are required for such exams, especially in later years.…”

Section: Introductionmentioning

confidence: 99%

Parents’ perceptions on previous and current science education system in Japan

Fomichova

Misonou

2018

JPBI

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Parents’ views, beliefs, and experiences greatly affect children’s attitudes toward education. The research aimed to identify the parents’ perception in the past, present, and their desired priorities in science education as well as investigated the adolescents’ perceptions based on gender and academic performance on science. As a survey study, this research relied on a questionnaire as the primary method of data collection which the data gained then was analysed by using statistical descriptive (percentage). The results showed that the priorities in education have shifted toward physics and chemistry in recent decades. Moreover, biology and earth science were found to be perceived as ‘easy’ and ‘secondary’ areas in comparison to the ‘superior’ ones (physics and chemistry). This has been strengthened by the critical situation for earth science, as the education system of Japan often does not al low the students to select it. Thus, the parents wished for a more balanced system. There was a difference in adolescents’ perceptions of science areas based on gender and academic performance. Physics and chemistry were associated with boys and top performers, while biology and earth science were supposed to ‘fit’ girls and low performers.

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Application of microRNA gene resources in the improvement of agronomic traits in rice

Cited by 50 publications

References 95 publications

Genome-wide small RNA profiling reveals tiller development in tall fescue (Festuca arundinacea Schreb.)

Genome-wide small RNA profiling reveals tiller development in tall fescue (Festuca arundinacea Schreb.)

Genome-wide Small Rna Profiling Reveals Tiller Development in Tall Fescue (Festuca Arundinacea Schreb.)

Parents’ perceptions on previous and current science education system in Japan

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