Application of microbially induced calcium carbonate precipitation with urea hydrolysis to improve the mechanical properties of soil

Naveed, Muhammad; Duan, Jiangong; Uddin, Shahab; Suleman, Muhammad; Ye, Hui; Li, Hongyu

doi:10.1016/j.ecoleng.2020.105885

Cited by 90 publications

(25 citation statements)

References 126 publications

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“…3. Effects of rock-solubilizing microbes on soil C and N content Soil urease activity characterizes the status of soil nitrogen [43,44]. Increased rhizospheric soil urease activities are positive for plants in terms of their utilization of soil nitrogen [45].…”

Section: Discussionmentioning

confidence: 99%

Rock-Solubilizing Microbial Inoculums Have Enormous Potential as Ecological Remediation Agents to Promote Plant Growth

Jia

Meng

et al. 2021

Forests

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Anthropogenic overexploitation poses significant threats to the ecosystems that surround mining sites, which also have tremendous negative impacts on human health and society safety. The technological capacity of the ecological restoration of mine sites is imminent, however, it remains a challenge to sustain the green restorative effects of ecological reconstruction. As a promising and environmentally friendly method, the use of microbial technologies to improve existing ecological restoration strategies have shown to be effective. Nonetheless, research into the mechanisms and influences of rock-solubilizing microbial inoculums on plant growth is negligible and the lack of this knowledge inhibits the broader application of this technology. We compared the effects of rock-solubilizing microbial inoculums on two plant species. The results revealed that rock-solubilizing microbial inoculums significantly increased the number of nodules and the total nodule volume of Robinia pseudoacacia L. but not of Lespedeza bicolor Turcz. The reason of the opposite reactions is possibly because the growth of R. pseudoacacia was significantly correlated with nodule formation, whereas L. bicolor’s growth index was more closely related to soil characteristics and if soil nitrogen content was sufficient to support its growth. Further, we found that soil sucrase activity contributed the most to the height of R. pseudoacacia, and the total volume of root nodules contributed most to its ground diameter and leaf area. Differently, we found a high contribution of total soil carbon to seedling height and ground diameter of L. bicolor, and the soil phosphatase activity contributed the most to the L. bicolor’ s leaf area. Our work suggests that the addition of rock-solubilizing microbial inoculums can enhance the supply capacity of soil nutrients and the ability of plants to take up nutrients for the promotion of plant growth. Altogether, our study provides technical support for the practical application of rock-solubilizing microbes on bare rock in the future.

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Section: Discussionmentioning

confidence: 99%

Rock-Solubilizing Microbial Inoculums Have Enormous Potential as Ecological Remediation Agents to Promote Plant Growth

Jia

Meng

et al. 2021

Forests

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“…[ 16 ]. Recent review work also figured out that versatile as this technique is, more joint efforts are required to evaluate the long-term performance under different environments and reduce the cost needed in the material and the process [ 17 , 18 ]. Existing research related to lowering the cost in MICP treatment focuses on mainly low-cost calcium resources [ 19 ], carbonate resources [ 20 , 21 ], and culture medium [ 22 , 23 ].…”

Section: Introductionmentioning

confidence: 99%

Experimental Study on Sand Stabilization Using Bio-Cementation with Wastepaper Fiber Integration

et al. 2021

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Recently, green materials and technologies have received considerable attention in geotechnical engineering. One of such techniques is microbially-induced carbonate precipitation (MICP). In the MICP process, CaCO3 is achieved bio-chemically within the soil, thus enhancing the strength and stiffness. The purpose of this study is to introduce the wastepaper fiber (WPF) onto the MICP (i) to study the mechanical properties of MICP-treated sand with varying WPF content (0–8%) and (ii) to assess the freeze–thaw (FT) durability of the treated samples. Findings revealed that the ductility of the treated samples increases with the increase in WPF addition, while the highest UCS is found with a small fiber addition. The results of CaCO3 content suggest that the WPF addition enhances the immobilization of the bacteria cells, thus yielding the precipitation content. However, shear wave velocity analysis indicates that a higher addition of WPF results in rapid deterioration of the samples when subjected to freeze–thaw cycles. Microscale analysis illuminates that fiber clusters replace the solid bonding at particle contacts, leading to reduced resistance to freeze–thaw damage. Overall, the study demonstrates that as a waste material, WPF could be sustainably reused in the bio-cementation.

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“…In the injection method, the silt particles in the sample were displaced by flow of bacteria suspension or reactant solution and caused heterogeneity. Naveed et al [29] stated that when the bacteria are injected within the soil, they are likely to be filtered within the soil particles with a reduction of bacterial population along the path of injection. Hence, it is difficult to accomplish the homogenous distribution of bacterial culture and cementation solution within the soil grains.…”

Section: Injection Of Reactant Solutionmentioning

confidence: 99%

Optimum Bacteria Suspension Volume for Stabilizing Silty Sand Soils by Sporosarcina pasteurii Bacteria

Karami

Shooshpasha

Alikhani

2022

IJE

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Recently, the bio-mediated soil improvement techniques have gained an increasing attention. In this method, the bacteria was cultivated aerobically in the laboratory and added to the soil with reactant solutions such as urea and calcium chloride. Most of the existing studies are on sandy soils and a few researches have done on silty sandy soils. However, most soils in nature are compounds of fine-grained and coarse-grained soils. In fine-grained soils, silt does not have very good resistance due to the lack of adhesion between its particles. Hence, in this study Sporosarcina pasteurii bacterium was aerobically cultivated for stabilizing the sand with different percentages of silt to determine the optimum bacteria suspension volume. After some bacterial tests such as measuring bacterial growth, standard plate count, gram staining, pH determination, growth without urea, and urease test, geo-technical tests like soil sieve, compaction, and Atterberg limits were also done. Standard plate count was estimated 2.5*10 8 through serial dilution plating and culture media pH was determined 8.64 from different samples. Moreover, to achieve the best results, different sampling methods were compared. As the calcium carbonate creates a network of calcified bridges of calcite between sand grains, an electron microscope was used for scanning the surface with a focused beam of electrons. Results of triaxial tests showed that by adding optimum bacteria suspension volume, the maximum strength for samples with 0, 10, 20, 30 and 40% of silt was improved from 700, 900, 750, 600 and550 to 1100, 1400, 1550, 1600, and 1500 kPa, respectively.

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Application of microbially induced calcium carbonate precipitation with urea hydrolysis to improve the mechanical properties of soil

Cited by 90 publications

References 126 publications

Rock-Solubilizing Microbial Inoculums Have Enormous Potential as Ecological Remediation Agents to Promote Plant Growth

Rock-Solubilizing Microbial Inoculums Have Enormous Potential as Ecological Remediation Agents to Promote Plant Growth

Experimental Study on Sand Stabilization Using Bio-Cementation with Wastepaper Fiber Integration

Optimum Bacteria Suspension Volume for Stabilizing Silty Sand Soils by Sporosarcina pasteurii Bacteria

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