Application of microbore HPLC in combination with tandem MS for the quantification of rosuvastatin in human plasma

Liquid chromatography/negative ion electrospray tandem mass spectrometry method for the quantification of rosuvastatin in human plasma: Application to a pharmacokinetic study

“…As a result, (−) ESI was chosen to obtain high sensitivity for rosuvastatin in this experiment. The LLOQ of 0.020 ng/ml was achieved under (−) ESI ionization mode, at least five times more sensitive than the reported methods [11][12][13][14].…”

“…However, the reported LC-MS/MS methods [11][12][13][14] for the quantification of rosuvastatin were all performed in positive ionization mode. During the initial method development, we also attempted to optimize ESI conditions under positive ionization mode.…”

“…In recent years, several methods including HPLC-UV [9,10] and LC-MS/MS [11][12][13][14] have been reported for the quantification of rosuvastatin in plasma. Most of the published LC-MS/MS methods [11][12][13][14] were based on positive electrospray detection, which provided a sensitivity at LLOQ of ≥0.1 ng/ml, using a plasma sample volume of 0.25-1.7 ml.…”

“…Most of the published LC-MS/MS methods [11][12][13][14] were based on positive electrospray detection, which provided a sensitivity at LLOQ of ≥0.1 ng/ml, using a plasma sample volume of 0.25-1.7 ml. The negative ionization mode was scarcely mentioned except one report in which rosuvastatin was served as internal standard (IS), where the spiked plasma concentration of rosuvastatin was 200 ng/ml [15].…”

Rapid measurement of deuterium-labeled long-chain fatty acids in plasma by HPLC-ESI-MS

“…As a first attempt, fatty acids were analyzed using multiple reaction monitoring (MRM) as an MS/MS approach to minimize interference from the plasma matrix, using C18:0 as a model compound for the optimization (21). As shown in Fig.…”