Application of microfluidic chips in the simulation of the urinary system microenvironment

Chao-zhen, Hou; Gu, Yubo; Yuan, Wei; Zhang, Wukai; Xiu, Xianjie; Lin, Jiahao; Gao, Yue; Liu, Peichuan; Chen, Xiang; Song, Lujie

doi:10.1016/j.mtbio.2023.100553

Cited by 4 publications

(2 citation statements)

References 139 publications

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“…Microfluidics has driven organ-on-a-chip from single-cell culture to membrane-based co-culture of two types of cells to multi-cell co-culture based on micro-pillar and 3D printing technologies [38]. During development, the microenvironment is enriched.…”

Section: Discussionmentioning

confidence: 99%

Construction of a three-dimensional urothelium on-chip with barrier function based on urinary flow microenvironment

Chao-zhen

Yuan

et al. 2023

Biofabrication

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The urothelium covers the inner surface of the urinary tract, forming a urinary tract barrier. Impairment of the integrity and dysfunction of the urinary tract barrier is associated with the occurrence and development of various diseases. The development of a three-dimensional model of the urothelium is critical for pathophysiological studies of this site, especially under physiological fluid shear stress stimulated by the urinary flow. In this study, a urothelium on-chip is fabricated with micromilling and replica molding techniques, which contains a microfluidic chip for cell culture and a pump-based fluid perfusion system. The mechanical properties of the human urinary tract are simulated by adjusting the concentration and degree of amino substitution of the gelatin methacrylate hydrogel. The matrix stiffness is similar to the natural urinary tract. Pulsatile flow and periodic flow are provided to simulate the fluid environment of the upper and lower urinary tracts, respectively. The results show that the physiological fluid shear stress could promote the differentiation and maturation of urothelial cells. The model could simulate the three-dimensional structure of urothelium and urinary flow microenvironment, showing morphological structure close to the natural urothelium, specific differentiation and maturation markers (uroplakin 2, cytokeratin 20), and urothelial barrier function.

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Section: Discussionmentioning

confidence: 99%

Construction of a three-dimensional urothelium on-chip with barrier function based on urinary flow microenvironment

Chao-zhen

Yuan

et al. 2023

Biofabrication

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“…The diversity of porous materials stems from a wide range of preparation methods [ 50 , 51 , 52 , 53 , 54 ]. Because of their controllable pore size and porosity, porous materials offer a wide range of applications for microfluidic systems [ 9 , 55 , 56 , 57 , 58 , 59 ].…”

Section: Different Materials and Preparation Methods For Microfluidic...mentioning

confidence: 99%

Porous Structural Microfluidic Device for Biomedical Diagnosis: A Review

et al. 2023

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Microfluidics has recently received more and more attention in applications such as biomedical, chemical and medicine. With the development of microelectronics technology as well as material science in recent years, microfluidic devices have made great progress. Porous structures as a discontinuous medium in which the special flow phenomena of fluids lead to their potential and special applications in microfluidics offer a unique way to develop completely new microfluidic chips. In this article, we firstly introduce the fabrication methods for porous structures of different materials. Then, the physical effects of microfluid flow in porous media and their related physical models are discussed. Finally, the state-of-the-art porous microfluidic chips and their applications in biomedicine are summarized, and we present the current problems and future directions in this field.

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