Application of microfluidic technology in antibody screening

Abstract

Specific antibodies are widely used in the biomedical field. Current screening methods for specific antibodies mainly involve hybridoma technology and antibody engineering techniques. However, these technologies suffer from tedious screening processes, long preparation periods, high costs, low efficiency, and a degree of automation, which have become a bottleneck for the screening of specific antibodies. To overcome these difficulties, microfluidics has been developed as a promising technology for high‐throughput screening and high purity of antibody. In this review, we provide an overview of the recent advances in microfluidic applications for specific antibody screening. In particular, hybridoma technology and four antibody engineering techniques (including phage display, single B cell antibody screening, antibody expression, and cell‐free protein synthesis) based on microfluidics have been introduced, challenges, and the future outlook of these technologies are also discussed.

Antibodies, repertoires and microdevices in antibody discovery and characterization

“…Other applications for FACSbased droplet sorting (also called fluorescent-activated droplet sorting (FADS)) include the generation of double emulsion pico-reactors. [97][98][99] In this workflow, cells are encapsulated twice to generate water-oil-water emulsions, which are more robust and can be used in aqueous systems such as FACS equipment. In contrast to sorting on chip, FADS allows for sorting-speeds common to cell-sorters with up to a few kHz.…”

Transforming Osteoarthritis Treatment: Embracing Hydrogel Microspheres

“…The application of microspheres depends on their properties, such as size, shape, and structure . Therefore, the utilization of microfluidic technology for the precise fabrication of microspheres through the manipulation of minute fluid volumes has gained widespread recognition in drug delivery systems, biomaterial synthesis, and analytical chemistry. − As illustrated in Figure C, apart from its resemblance to other methodologies, the essence of this technology lies in the development of a diverse range of miniature devices that possess the capability to meet specific requirements for droplet formation. The two primary devices currently employed are capillary microfluidic devices and lithographically fabricated poly­(dimethylsiloxane) (PDMS) devices.…”

Droplets microfluidics platform—A tool for single cell research

“…Based on this, many scientists have studied the molecular background of cancer immune checkpoints and made discoveries related to cancer immune surveillance ( Wuethrich et al, 2019 ; Strati et al, 2021 ). In addition, microfluidics will eventually allow the screening of highly specific therapeutic antibodies from human B cells (in cancer patients and disease survivors) ( Broketa and Bruhns, 2022 ; Sun et al, 2022 ). As antibodies of human origin, these antibodies should be free of any adverse reactions and side effects, and due to their high specificity, the therapeutic effect should be significant.…”