Application of microsatellite markers as potential tools for traceability of Girgentana goat breed dairy products

Sardina, Maria Teresa; Tortorici, Lina; Mastrangelo, Salvatore; Gerlando, Rosalia Di; Tolone, Marco; Portolano, Baldassare

doi:10.1016/j.foodres.2015.04.038

Cited by 36 publications

(21 citation statements)

References 28 publications

(37 reference statements)

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“…At one level, mitochondrial genes such as COI, used for DNA barcoding, cytochrome b , and 12S and 16S rRNA, are used to identify species, whereas populations can be distinguished according to their allelic frequencies using probabilistic or deterministic approaches . The identification of individuals is based on parental screening . In aquatic species, individual tracing and the identification of populations are often based on microsatellites, which are highly polymorphic and informative, and are present throughout the whole genome …”

Section: Introductionmentioning

confidence: 99%

“…2,18 The identification of individuals is based on parental screening. 2,19 In aquatic species, individual tracing and the identification of populations are often based on microsatellites, which are highly polymorphic and informative, and are present throughout the whole genome. 4,20,21 A number of methods and analytical procedures have been applied to individual tracing and identification of populations.…”

Section: Introductionmentioning

confidence: 99%

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Tracing individuals and populations of the tambaqui, Colossoma macropomum (Cuvier, 1818), from Brazilian hatcheries using microsatellite markers

et al. 2019

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BACKGROUND In recent years, tracing of alimentary produce of animal origin has become increasingly important, for economic, food safety and ecological reasons. The tambaqui, Colossoma macropomum, is the native fish most farmed in Brazil. The reliable identification of the origin of tambaquis (wild or farmed) offered for sale to the general public has become necessary to satisfy regulatory norms and uphold consumer confidence. Molecular methods based on the analysis of DNA sequences have often been used to evaluate the potential for tracing farmed fish, given their reliability and precision. RESULTS Full likelihood and Bayesian approaches proved to be the most efficient for the identification, respectively, of individuals and populations for most of the fish sampled from seven hatcheries and one wild stock. The exclusion method and genetic distances were the least effective approaches for the identification of individuals and populations. The Bayesian method identified correctly more than 99% of the fry from most stocks, except those of the Santarém hatchery and River Amazon wild stock, which presented the best results for individual identification. CONCLUSIONS The identification of populations was effective for most hatcheries, although the identification of individuals from most stocks was hampered by the reduced genetic variability. © 2018 Society of Chemical Industry

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Tracing individuals and populations of the tambaqui, Colossoma macropomum (Cuvier, 1818), from Brazilian hatcheries using microsatellite markers

et al. 2019

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“…In livestock, breed discrimination is useful to detect fraud and to protect and valorize typical productions. Girgentana goat (Capra hircus L.), an ancient breed reared in a restricted area of Sicily (southern Italy) and its dairy products were traced by the use of a specific panel of microsatellites [26]. The potential of microsatellites for determining the origin of meat products was also important for traceability of nine Portuguese breeds with PDO products [27], while four Italian cattle breeds were identified by microsatellite markers using different statistical approaches to certify the origin of their typical products [28].…”

Section: Microsatellites Have a Series Of Characteristics That Make Tmentioning

confidence: 99%

Use of Microsatellites to Study Agricultural Biodiversity and Food Traceability

Bernardi¹,

Colli²,

Ughini³

et al. 2016

Microsatellite Markers

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Molecular markers are useful tools for measuring the genetic diversity among agricultural species. In plants, microsatellites are still the most used markers for germplasm characterization, conservation, and traceability purposes, while in the livestock sector, although having represented the standard for at least two decades, they are still used only for minor farm animal species. In this work, together with a review on the use of microsatellites in livestock, we also illustrate the use of these markers for the characterization of agricultural diversity and food traceability through two case studies: (i) the analysis of genetic diversity in ancient fruit tree cultivars of apple (Malus × domestica Borkh.), pear (Pyrus communis L.), sweet cherry (Prunus avium L.), and sour cherry (Prunus cerasus L.) from Northern Italy and (ii) the molecular authentication of wheat food chain. In the former case, a high genetic variability as well as the presence of different ploidy levels were detected, while in the latter microsatellite markers were shown to be useful for traceability and product authentication along the whole food chain. Overall, the presented evidence confirms the versatility of microsatellites as markers for both agrobiodiversity characterization and food traceability in cultivated plants and farm animals.

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“…Figure 1 shows that DNA barcoding became a keyword in the scientific literature since 2000, hence it must be considered a very recent topic in the research field around the world. Applications of DNA barcoding to food safety and food piracy issues have grown in importance due to the consumers' increasing attention to food authenticity and food safety in different products and species [24][25][26][27]. This interest seems to be due to the consumers' lack of confidence, in particular towards food of animal origin, and it is attributable to several reasons including both food safety and socio-economic changes.…”

Section: Dna Barcoding and The Food Production Chainsmentioning

confidence: 99%

DNA Barcoding as a Molecular Tool to Track Down Mislabeling and Food Piracy

2015

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DNA barcoding is a molecular technology that allows the identification of any biological species by amplifying, sequencing and querying the information from genic and/or intergenic standardized target regions belonging to the extranuclear genomes. Although these sequences represent a small fraction of the total DNA of a cell, both chloroplast and mitochondrial barcodes chosen for identifying plant and animal species, respectively, have shown sufficient nucleotide diversity to assess the taxonomic identity of the vast majority of organisms used in agriculture. Consequently, cpDNA and mtDNA barcoding protocols are being used more and more in the food industry and food supply chains for food labeling, not only to support food safety but also to uncover food piracy in freshly commercialized and technologically processed products. Since the extranuclear genomes are present in many copies within each cell, this technology is being more easily exploited to recover information even in degraded samples or transformed materials deriving from crop varieties and livestock species. The strong standardization that characterizes protocols used worldwide for DNA barcoding makes this technology particularly suitable for routine analyses required by agencies to safeguard food safety and quality. Here we conduct a critical review of the potentials of DNA barcoding for food labeling along with the main findings in the area of food piracy, with particular reference to agrifood and livestock foodstuffs.

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Application of microsatellite markers as potential tools for traceability of Girgentana goat breed dairy products

Cited by 36 publications

References 28 publications

Tracing individuals and populations of the tambaqui, Colossoma macropomum (Cuvier, 1818), from Brazilian hatcheries using microsatellite markers

Tracing individuals and populations of the tambaqui, Colossoma macropomum (Cuvier, 1818), from Brazilian hatcheries using microsatellite markers

Use of Microsatellites to Study Agricultural Biodiversity and Food Traceability

DNA Barcoding as a Molecular Tool to Track Down Mislabeling and Food Piracy

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