Application of Migration Inhibition Techniques in Tumor Immunology

Szigeti, Robert

doi:10.1016/s0065-230x(08)60948-3

Cited by 14 publications

(5 citation statements)

References 198 publications

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“…The c DNA of one of these has recently been cloned and the sequence is not homologous to that described for other cytokines [13]. MIF activity has been corre lated extensively with immunological responses in cancer [14] and other human diseases [15,16] and ap pears to be important in the expression of delayed hy persensitivity [17]. Factors stimulating macrophage migration (MStF) block the effect of MIF on macro phage migration and are associated with reduced im munological responsiveness [18,19].…”

Section: Discussionmentioning

confidence: 99%

Manipulation of Macrophage Migration Inhibition/Stimulation Responses by Adjuvants and Interleukins

MacSween¹,

Rajaraman²,

Rajaraman³

1990

Int Arch Allergy Immunol

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C57 B1/6 mice were immunized with bovine serum albumin (BSA) and Freund’s complete and incomplete adjuvants in various concentrations. Spleen cells from these animals were subsequently stimulated with concanavalin A (Con A), purified protein derivative or BSA, and lymphokine responses were measured in one-stage migration assays. Con A consistently produced macrophage migration inhibition factor (MIF) responses in nonimmunized animals and those immunized with complete adjuvant. This was switched to migration stimulation factor (MStF) responses by prior immunization with incomplete adjuvant. Immunization with complete adjuvant and with BSA alone was followed by MIF responses to antigenic stimulation whereas incomplete adjuvant promoted MStF responses. The MStF responses to both mitogenic and antigenic stimulation were inhibited by the addition of affinity purified interleukin-1 to the spleen cells. Interleukin-1 also inhibited MStF responses and potentiated MIF responses to Con A stimulation by human mononuclear cells whereas interleukin-2 did not influence these responses

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Section: Discussionmentioning

confidence: 99%

Manipulation of Macrophage Migration Inhibition/Stimulation Responses by Adjuvants and Interleukins

MacSween¹,

Rajaraman²,

Rajaraman³

1990

Int Arch Allergy Immunol

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“…At the indicated time points 100 p1 cell-free supernatants were collected from the different cultures. Supernatants from triplicate cultures were pooled and tested for their ability to inhibit the random migration of purified granulocytes in the indirect LMI assay [28]. Each supernatant was tested in triplicate.…”

Section: Lmi Assaymentioning

confidence: 99%

Activation of B lymphocytes by Epstein‐Barr virus/CR2 receptor interaction

et al. 1987

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Epstein-Barr virus (EBV) infects and transforms human B lymphocytes. The virus receptor was shown to be identical to the complement receptor CR2. The consequences of EBV/CR2 receptor interaction on B lymphocyte activation were analyzed by infection of B cells with the transforming (B95-8) and the nontransforming (P3HR1) virus strains and with UV-inactivated B95-8 virus. Similar to mitogens and antibodies against surface IgM and CR2 receptor, transforming and nontransforming EBV induced the release of leukocyte migration inhibitory factor (LIF). LIF production occurred early after infection and was not affected by irradiation of the B95-8 virus with doses of UV-light which prevented the expression of viral functions. B lymphocytes infected with UV-inactivated virus responded to T cell-derived B cell growth factors. The results demonstrate that binding of EBV to the CR2 receptor activates the B cells. Progression of the infected cells in the activation pathway required helper cell-derived factors or signals provided by the intact viral genome. The nontransforming P3HR1 virus induced a low level of RNA synthesis and increase of cell size and major histocompatibility complex class I antigen expression. Only the transforming virus induced expression of EBV nuclear antigens and cellular DNA synthesis.

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“…Migration index (MI) was calculated according to the formula MI = mean of triplicates with antigenhean of triplicates without antigen. Upper and lower cut-off values of the non-reactive range (= negative LMI reaction) were calculated from the MI values obtained (mean f 2 SD) with cells from EBV seronegative subjects and extracts from EBV-genome-carrying P3HR-1 and Raji cells (Szigeti et al, 1981(Szigeti et al, , 1982Szigeti, 1985). A lower borderline MI value of 0.78 was obtained.…”

Section: Leukocyte Migration Inhibitionmentioning

confidence: 99%

Stable transfection of a human lymphoma line by sub‐genomic fragments of Epstein‐Barr virus DNA to measure humoral and cellular immunity to the corresponding proteins

Welinder

Larsson

Szigeti

et al. 1987

Intl Journal of Cancer

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An Epstein-Barr virus (EBV)-negative human lymphoid B-cell line, DG75, was stably transfected with recombinant selection vectors that carry a subfragment of the BamHI WYH region (nucleotides 44664 to 50628), the BamHI K fragment, or a subfragment of the EcoRI D region (nucleotides 166614 to 170149) of B95-8 EBV DNA. These fragments contain the coding exons for the EBV-determined nuclear antigens EBNA2 and EBNA1, and the membrane antigen LMP, respectively. Antigen expression of the cells was detected by immunofluorescence. EBNA2 was expressed in 80-100% of the transfected cells, in contrast to EBNA1 which was expressed in only 25%, and LMP in only about 5% of the cells. Humoral antibody responses were measured by immunofluorescence and compared to cellular immunity as determined by the leukocyte migration inhibition (LMI) technique. Extracts from transfected cell lines expressing EBNA1, EBNA2 or LMP elicited an LMI response with cells from healthy EBV-seropositive individuals whereas the extract from the parental DG75 cell line did not. The results demonstrate the value of stably transfected cell lines expressing a defined EBV antigen for the monospecific analysis of host responses to the EBV-encoded antigen complex in growth-transformed cells.

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Application of Migration Inhibition Techniques in Tumor Immunology

Cited by 14 publications

References 198 publications

Manipulation of Macrophage Migration Inhibition/Stimulation Responses by Adjuvants and Interleukins

Manipulation of Macrophage Migration Inhibition/Stimulation Responses by Adjuvants and Interleukins

Activation of B lymphocytes by Epstein‐Barr virus/CR2 receptor interaction

Stable transfection of a human lymphoma line by sub‐genomic fragments of Epstein‐Barr virus DNA to measure humoral and cellular immunity to the corresponding proteins

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