Application of modified in vitro screening procedure for identifying herbals possessing sulfonylurea-like activity

Rotshteyn, Yakov; Zito, S. William

doi:10.1016/j.jep.2004.04.007

Cited by 45 publications

(27 citation statements)

References 13 publications

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“…DM is associated with increased risk of heart disease, stroke, kidney disease, retinopathy, neuropathy, ulceration and gangrene of extremities (Rotshteyn and Zito, 2004). Thus, DM and its associated complications have significant impact on health, quality of life and life expectancy of its sufferers.…”

Section: Introductionmentioning

confidence: 99%

Pharmacological evaluation of oral hypoglycemic and antidiabetic effects of fresh leaves ethanol extract of morinda lucida benth. in normal and alloxan- induced diabetic rats

Adeneye¹,

Agbaje²

2010

African Journal of Biomedical Research

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Section: Introductionmentioning

confidence: 99%

Pharmacological evaluation of oral hypoglycemic and antidiabetic effects of fresh leaves ethanol extract of morinda lucida benth. in normal and alloxan- induced diabetic rats

Adeneye¹,

Agbaje²

2010

African Journal of Biomedical Research

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“…34) Moreover, ginseng treatment increased insulin release from pancreatic β-cells, which is probably caused by increasing β-cell stimulation and insulin synthesis. [35][36][37] Ginseng consists of a number of active constituents, such as polysaccharides, peptides, polyacetylenic alcohols and fatty acids, 38) of these, and most pharmacological properties are mainly attributed to ginsenosides. 39) Ginsenosides Rb1 and Rg1 hold important positions in stimulatory and inhibitory effects on central nervous system.…”

mentioning

confidence: 99%

Anti-apoptotic Function and Mechanism of Ginseng Saponins in Rattus Pancreatic β-Cells

Chen

Kang

et al. 2012

Biological & Pharmaceutical Bulletin

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Apoptosis is the main form of β-cell death in diabetes. Ginseng has been used as an anti-diabetic herb for several thousand years in Asia with ginsenoside Rg1 and ginsenoside Rb1 as important active ingredients. In this study, we demonstrated ginsenoside Rg1 and Rb1 protect β-cells from high glucose/cytokine-induced pancreatic β-cell apoptosis via inhibiting nitric oxide (NO) production and regulating apoptosis-related genes. Among these genes, Bax, Fas and Caspase-3 gene expressions were up-regulated by high glucose, whereas only Bax and Caspase-3 gene expression were elevated by cytokines. In these two stimuli-induced apoptotic cells, Rg1 down-regulated Fas gene expression, while Rb1 decreased Caspase-3 gene expression. As a conclusion, Fas signal pathway and mitochondria stress is mostly related to anti-diabetes function of ginsenoside Rg1, while Caspase-3 pathway is essential when Rb1 is present.

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“…Thus, it is reasonable to consider that GLP1 as well as b-cell mass markedly increased in the DM-GH group while insulin content in plasma and pancreas just increased moderately. In addition, enhanced insulin level may have appeared obviously due to the insulinotropic action of GLP1 as well as ginsenosides (Kimura et al 1981b, Rotshteyn & Zito 2004, Park et al 2008b) during the treatment.…”

Section: Discussionmentioning

confidence: 99%

Increased glucagon-like peptide-1 secretion may be involved in antidiabetic effects of ginsenosides

Liu

Zhang

et al. 2013

Journal of Endocrinology

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Panax ginseng is one of the most popular herbal remedies. Ginsenosides, major bioactive constituents in P. ginseng, have shown good antidiabetic action, but the precise mechanism was not fully understood. Glucagon-like peptide-1 (GLP1) is considered to be an important incretin that can regulate glucose homeostasis in the gastrointestinal tract after meals. The aim of this study was to investigate whether ginseng total saponins (GTS) exerts its antidiabetic effects via modulating GLP1 release. Ginsenoside Rb1 (Rb1), the most abundant constituent in GTS, was selected to further explore the underlying mechanisms in cultured NCI-H716 cells. Diabetic rats were developed by a combination of high-fat diet and low-dose streptozotocin injection. The diabetic rats orally received GTS (150 or 300 mg/kg) daily for 4 weeks. It was found that GTS treatment significantly ameliorated hyperglycemia and dyslipidemia, accompanied by a significant increase in glucose-induced GLP1 secretion and upregulation of proglucagon gene expression. Data from NCI-H716 cells showed that both GTS and Rb1 promoted GLP1 secretion. It was observed that Rb1 increased the ratio of intracellular ATP to ADP concentration and intracellular Ca 2C concentration. The metabolic inhibitor azide (3 mM), the K ATP channel opener diazoxide (340 mM), and the Ca 2C channel blocker nifedipine (20 mM) significantly reversed Rb1-mediated GLP1 secretion. All these results drew a conclusion that ginsenosides stimulated GLP1 secretion both in vivo and in vitro. The antidiabetic effects of ginsenosides may be a result of enhanced GLP1 secretion. Key Words" glucagon-like peptide-1 (GLP1)" ginsenosides " Rb1" type 2 diabetes " ginseng total saponins (GTS)

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Application of modified in vitro screening procedure for identifying herbals possessing sulfonylurea-like activity

Cited by 45 publications

References 13 publications

Pharmacological evaluation of oral hypoglycemic and antidiabetic effects of fresh leaves ethanol extract of morinda lucida benth. in normal and alloxan- induced diabetic rats

Pharmacological evaluation of oral hypoglycemic and antidiabetic effects of fresh leaves ethanol extract of morinda lucida benth. in normal and alloxan- induced diabetic rats

Anti-apoptotic Function and Mechanism of Ginseng Saponins in Rattus Pancreatic β-Cells

Increased glucagon-like peptide-1 secretion may be involved in antidiabetic effects of ginsenosides

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