Application of Molecular Genetics and Transformation to Barley Improvement

Thomas, William; Hayes, Patrick; Dahleen, Lynn S.

doi:10.1002/9780470958636.ch6

Cited by 4 publications

(3 citation statements)

References 106 publications

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“…The ultimate goal of GWAS is to identify gene alleles that control phenotypic diversity in breeding pools ( Thomas et al , 2011 ). In classical SNP-based bi-parental QTL approaches only two alleles can be distinguished.…”

Section: Discussionmentioning

confidence: 99%

Contrasting genetic regulation of plant development in wild barley grown in two European environments revealed by nested association mapping

Herzig

Maurer

Draba

et al. 2018

Journal of Experimental Botany

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Section: Discussionmentioning

confidence: 99%

Contrasting genetic regulation of plant development in wild barley grown in two European environments revealed by nested association mapping

Herzig

Maurer

Draba

et al. 2018

Journal of Experimental Botany

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“…We assembled a collection of 209 European two-rowed spring barley cultivars (Supplemental Table 1), which is a representative subset of previously described two-row spring European barley populations 10,11,17,19 that show a significant increase in yield over time. Pedigree data was collected from publications 17–20 , and the following two websites: https://grinczech.vurv.cz/gringlobal/search.aspx and https://www.lfl.bayern.de/mam/cms07/ipz/dateien/abst_gerste.pdf.…”

Section: Methodsmentioning

confidence: 99%

“…The 209 two-row spring barley population was selected from previously established datasets containing 647 genotypes 10,11,17–19 . To include a wide range of genetically representative individuals, we used available BOPA SNP data (as previously described) 50 and did a multi-dimensional scaling plot (Figure 1 a).…”

Section: Technical Validationmentioning

confidence: 99%

Genomic resources for a historical collection of cultivated two-row European spring barley genotypes

Schreiber

Wonneberger

Haaning

et al. 2023

Preprint

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Barley genomic resources are increasing rapidly, with the publication of a barley pangenome as one of the latest developments. Two-row spring barley cultivars are intensely studied as they are the source of high-quality grain for malting and distilling. Here we provide data from a European two-row spring barley population containing 209 different genotypes registered for the UK market between 1830 to 2014. The dataset encompasses RNA-sequencing data from six different tissues across a range of barley developmental stages, phenotypic datasets from two consecutive years of field-grown trials in the United Kingdom, Germany and the USA; and whole genome shotgun sequencing from all cultivars, which was used to complement the RNA-sequencing data for variant calling. The outcomes are a filtered SNP marker file, a phenotypic database and a large gene expression dataset providing a comprehensive resource which allows for downstream analyses like genome wide association studies or expression associations.

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Genetic Transformation of Hordeum vulgare ssp. spontaneum for the Development of a Transposon-Based Insertional Mutagenesis System

2016

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Domestication and intensive selective breeding of plants has triggered erosion of genetic diversity of important stress-related alleles. Researchers highlight the potential of using wild accessions as a gene source for improvement of cereals such as barley, which has major economic and social importance worldwide. Previously, we have successfully introduced the maize Ac/Ds transposon system for gene identification in cultivated barley. The objective of current research was to investigate the response of Hordeum vulgare ssp. spontaneum wild barley accessions in tissue culture to standardize parameters for introduction of Ac/Ds transposons through genetic transformation. We investigated the response of ten wild barley genotypes for callus induction, regenerative green callus induction and regeneration of fertile plants. The activity of exogenous Ac/Ds elements was observed through a transient assay on immature wild barley embryos/callus whereby transformed embryos/calli were identified by the expression of GUS. Transient Ds expression bombardment experiments were performed on 352 pieces of callus (3-5 mm each) or immature embryos in 4 genotypes of wild barley. The transformation frequency of putative transgenic callus lines based on transient GUS expression ranged between 72 and100 % in wild barley genotypes. This is the first report of a transformation system in H. vulgare ssp. spontaneum.

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Application of Molecular Genetics and Transformation to Barley Improvement

Cited by 4 publications

References 106 publications

Contrasting genetic regulation of plant development in wild barley grown in two European environments revealed by nested association mapping

Contrasting genetic regulation of plant development in wild barley grown in two European environments revealed by nested association mapping

Genomic resources for a historical collection of cultivated two-row European spring barley genotypes

Genetic Transformation of Hordeum vulgare ssp. spontaneum for the Development of a Transposon-Based Insertional Mutagenesis System

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