Application of Molecular Marker-Assisted Selection (MAS) for Disease Resistance in a Practical Potato Breeding Programme

Ortega, Felisa; Lopez-Vizcon, Claudia

doi:10.1007/s11540-011-9202-5

Cited by 63 publications

(36 citation statements)

References 18 publications

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“…for cyst nematode and Potato virus Y (Ortega and Lopez-Vizcon 2012;Whitworth et al 2009). It also looks promising for tuber quality features (Li et al, 2013).…”

Section: Specific Breeding Toolsmentioning

confidence: 99%

Overview and Breeding Strategies of Table Potato Production in Sweden and the Fennoscandian Region

et al. 2016

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Recent reductions in the public commitment to potato breeding in Sweden, Norway and Finland call for an evaluation of the current situation regarding the commercial basis for, and structure of, potato breeding in these countries. We here review the extent of cultivation, processing and consumption of table potato in Sweden, as well as provide an overview of the potato breeding tools and programmes in the three countries. We then discuss various strategies to provide long-term stability and increase the impact of public potato breeding, based on the similar overall conditions for potato cultivation across the Fennoscandian region. The conclusions are twofold; first, an increased long-term funding of the public potato breeding programmes is necessary to maintain a minimum level of material, and second, a coordination of the breeding activities in the Fennoscandian region would be of great benefit to all involved stakeholders and allow an enhancement of the current national breeding programmes. In addition, we propose a minimum first field year population size for potato breeding.

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“…for cyst nematode and Potato virus Y (Ortega and Lopez-Vizcon 2012;Whitworth et al 2009). It also looks promising for tuber quality features (Li et al, 2013).…”

Section: Specific Breeding Toolsmentioning

confidence: 99%

Overview and Breeding Strategies of Table Potato Production in Sweden and the Fennoscandian Region

et al. 2016

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“…To implement MAS for PVY resistance in the Oregon Potato Breeding Program, the RYSC3 marker and the CAPS marker ADG2 were used and confirmed as tools to identify PVY-resistant clones (Ottoman et al 2009). Appacale (Spain), a public breeding company, used RYSC3 and STM0003 markers to select breeding clones resistant to PVY (Ortega and Lopez-Vizcon 2012). The SCAR markers RYSC3 and YES3-3 were used for application of MAS in a conventional potato breeding program in the University of Wisconsin, USA (Fulladolsa et al 2015).…”

Section: Introductionmentioning

confidence: 99%

Molecular and genetic characterization of the Ryadg locus on chromosome XI from Andigena potatoes conferring extreme resistance to potato virus Y

et al. 2018

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Key messageWe have elucidated the Andigena origin of the potato Ryadg gene on chromosome XI of CIP breeding lines and developed two marker assays to facilitate its introgression in potato by marker-assisted selection.AbstractPotato virus Y (PVY) is causing yield and quality losses forcing farmers to renew periodically their seeds from clean stocks. Two loci for extreme resistance to PVY, one on chromosome XI and the other on XII, have been identified and used in breeding. The latter corresponds to a well-known source of resistance (Solanum stoloniferum), whereas the one on chromosome XI was reported from S. stoloniferum and S. tuberosum group Andigena as well. To elucidate its taxonomic origin in our breeding lines, we analyzed the nucleotide sequences of tightly linked markers (M45, M6) and screened 251 landraces of S. tuberosum group Andigena for the presence of this gene. Our results indicate that the PVY resistance allele on chromosome XI in our breeding lines originated from S. tuberosum group Andigena. We have developed two marker assays to accelerate the introgression of Ryadg gene into breeding lines by marker-assisted selection (MAS). First, we have multiplexed RYSC3, M6 and M45 DNA markers flanking the Ryadg gene and validated it on potato varieties with known presence/absence of the Ryadg gene and a progeny of 6,521 individuals. Secondly, we developed an allele-dosage assay particularly useful to identify multiplex Ryadg progenitors. The assay based on high-resolution melting analysis at the M6 marker confirmed Ryadg plex level as nulliplex, simplex and duplex progenitors and few triplex progenies. These marker assays have been validated and can be used to facilitate MAS in potato breeding.

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“…The utilization of the RYSC3 marker has been validated by many authors such as Dalla Rizza et al (2006), Sagredo et al (2009), Veramendi et al (2011), Ortega and Lopez-Vizcon (2012), and Lopez-Pardo et al (2013. In the evaluation of the 271 Solanum tuberosum subsp.…”

Section: Discussionmentioning

confidence: 99%

“…These represent a 95% correlation between molecular and serological results. Both methods are usually applied as a tool for virus resistances evaluations; like Gebhardt et al (2006), Sagredo et al (2009), andOrtega andLopez-Vizcon (2012). The remaining 148 accessions showed no virus infection and no molecular marker amplification, task that might be explained by unknown PVY resistance sources, since that plant has been exposed to natural pest presence on the field.…”

Section: Discussionmentioning

confidence: 99%

Identification of virus and nematode resistance genes in the Chilota Potato Genebank of the Universidad Austral de Chile

López

Riegel

Lizana

et al. 2015

Chilean J. Agric. Res.

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Potato Genebank of the Universidad Austral de Chile (UACh) is an important gene bank in Chile. The accessions collected all over the country possess high genetic diversity, present interesting agronomic and cooking traits, and show resistance to biotic and abiotic stress. A particularly interesting subgroup of the gene bank includes the accessions collected in the South of Chile, the Chilota Potato Genebank. The focus of this study is the identification of virus and nematode resistant genes in potatoes (Solanum tuberosum L.), using the RYSC3 and YES3-3B molecular markers. The Potato virus Y (PVY) resistance genes Ry adg and Ry sto were identified. Furthermore, the CP60 marker was used to assess the Rx resistance gene that confers resistance to Potato virus X (PVX). In addition, the HC and GRO1-4 markers were utilized to identify the GpaV vrn _QTL and Gro1-4, resistance genes of Globodera pallida and Globodera rostochiensis, respectively. Both G. pallida and G. rostochiensis are Potato Cyst Nematodes (PCN). The plant material used in this study included leaves from 271 accessions of the gene bank. These samples were collected in the field where natural pathogen pressure of potential viruses and diseases exists. ELISA assays were run for field detection of PVY and PVX. However, there have been no previous reports of nematode presence in the plant material. The results herein presented indicate presence of virus and nematode resistance genes in accessions of the Chilota Potato Genebank. In terms of virus resistance, 99 accessions out of the 271 tested possess the Ry adg resistance gene and 17 accessions of these 271 tested have the Ry sto resistance gene. Also, 10 accessions showed positive amplification of the Rx1 resistant gene marker. As to nematode resistance, 99 accessions have possible resistance to G. pallida and 54 accessions show potential resistance to G. rostochiensis as detected using the available molecular markers.

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Application of Molecular Marker-Assisted Selection (MAS) for Disease Resistance in a Practical Potato Breeding Programme

Cited by 63 publications

References 18 publications

Overview and Breeding Strategies of Table Potato Production in Sweden and the Fennoscandian Region

Overview and Breeding Strategies of Table Potato Production in Sweden and the Fennoscandian Region

Molecular and genetic characterization of the Ryadg locus on chromosome XI from Andigena potatoes conferring extreme resistance to potato virus Y

Identification of virus and nematode resistance genes in the Chilota Potato Genebank of the Universidad Austral de Chile

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