Application of molecular, microbiological, and immunological tests for the diagnosis of bone and joint tuberculosis

Tang, Yinghua; Yin, Lianli; Tang, Shifu; Zhang, Hongyu; Lan, Jihui

doi:10.1002/jcla.22260

Cited by 21 publications

(16 citation statements)

References 32 publications

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“…In the patients with NIH IIIb, the PSEP cut-off of 1.00 ng/mL corresponds to a detection sensitivity of 69% and a specificity of 70%, suggesting that PSEP as a clinical test for CP has operability and good clinical value. In addition, previous studies have suggested that TNF-α, as a multifunctional cytokine, is involved in the pathogenesis of various diseases [32] . PSEP is only reported in prostate diseases and has excellent specificity.…”

Section: Discussionmentioning

confidence: 99%

The application of IL-10 and TNF-α in expressed prostatic secretions and prostatic exosomal protein in urine in the diagnosis of patients with chronic prostatitis

et al. 2019

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Background:The aim of this study was to investigate the expression of tumor necrosis factor alpha (TNF-α) and interleukin-10 (IL-10) in expressed prostatic secretions (EPSs) of patients with chronic prostatitis (CP) and the expression of prostatic exosomal protein (PSEP) in urine, and to evaluate its correlation with the condition.Methods:Urine samples from 310 patients with CP (101 National Institutes of Health [NIH] II, 112 NIH IIIa, and 97 NIH IIIb, classified according to the US National Institutes of Health) and 110 control group subjects were collected. The samples were tested for PSEP by enzyme-linked immunosorbent assay (ELISA). At the same time, EPSs in 60 patients from 310 patients with CP and 20 control group subjects were collected. The levels of IL-10 and TNF-α in the collected samples that EPS were determined by double antibody sandwich ELISA. SPSS 23.0 statistical software was used for statistical analysis of the measured data.Results:The level of PSEP in patients with CP was significantly higher than that in the control group (P < .001). The levels of TNF-α and IL-10 in the EPS of patients with NIH II and NIH IIIa CP were higher than those of the patients with NIH IIIb and the control group (P < .001). There was a positive correlation between PSEP and IL-10 and TNF-α, while TNF-α and IL-10 were also positively correlated.Conclusion:PSEP, TNF-α, and IL-10 may serve as a basis for the classification diagnosis of CP. Their combination can provide more accurate diagnostic information for clinical CP typing.

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Section: Discussionmentioning

confidence: 99%

The application of IL-10 and TNF-α in expressed prostatic secretions and prostatic exosomal protein in urine in the diagnosis of patients with chronic prostatitis

et al. 2019

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“…Tuberculosis is a major infectious disease that mainly affects lung tissue, but may also infect other organs. The infection can be spread by two major routes: hematogenous or lymphatic [12].…”

Section: Discussionmentioning

confidence: 99%

Tuberculous Extensor Tenosynovitis Presenting as Huge Mass on the Dorsum of the Hand

Mansour

Moussa

Khalaf

et al. 2020

Cureus

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Infectious tuberculous tenosynovitis (TS) of the extensor tendons of the wrist is an exceptional location of musculoskeletal tuberculosis. We present a case of tuberculous extensor TS in a 52year-old diabetic male patient presenting as a huge mass on the dorsum of the hand, in the absence of other pulmonary or extrapulmonary manifestation of tuberculosis. This report increases physicians' vigilance when dealing with patients with risk factors of tuberculosis, allowing early diagnosis and optimal treatment.

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“…The diagnosis of BJTB, just like that of other forms of EPTB, is very challenging, due to its paucibacillary nature [48]. The delayed diagnosis and treatment of BJTB can lead to serious complications, such as joint destruction, contractures in large joints, growth arrest, and neurological impairment in spinal tuberculosis, resulting in long-term morbidity and disability [36]. Invasive examination is a necessary diagnostic step in most cases.…”

Section: Discussionmentioning

confidence: 99%

Diagnostic accuracy of the Xpert MTB/RIF assay for bone and joint tuberculosis: A meta-analysis

Shen¹,

Yu²,

Zhong³

et al. 2019

PLoS ONE

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BackgroundThis study aimed to evaluate the accuracy of the Xpert MTB/RIF assay for the diagnosis of bone and joint tuberculosis. MethodsWe searched databases from their inception to May 7, 2019 for published articles and reviewed them to assess the accuracy of Xpert MTB/RIF with respect to a composite reference standard (CRS) and mycobacterial culture. Meta-analyses were performed using a bivariate random-effects model, and the sources of heterogeneity were assessed via subgroup analysis and meta-regression. ResultsNineteen independent (9 prospective, 5 retrospective, and 5 case-control) studies that compared Xpert MTB/RIF with the CRS and 14 (6 prospective, 7 retrospective, and 1 case-control) studies that compared it with culture were included. The pooled sensitivity and specificity of Xpert MTB/RIF were 81% (95% confidence interval [CI], 77-84) and 99% (95% CI, 97-100) compared to the CRS, respectively, and 96% (95% CI, 90-98) and 85% (95% CI, 57-96) compared to culture, respectively. The pooled sensitivity and specificity using pus samples vs. the CRS were 82% (95% CI, 76-86) and 99% (95% CI, 95-100), respectively. The proportions obtained while working with tissue samples vs. the CRS were 84% (95% CI, 76-90) and 98% (95% CI, 94-99), respectively. There was no significant difference in diagnostic accuracy among the types of specimens. ConclusionsXpert MTB/RIF demonstrates good diagnostic accuracy for bone and joint tuberculosis, the results of which are not related to the type of specimen.

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Application of molecular, microbiological, and immunological tests for the diagnosis of bone and joint tuberculosis

Abstract: Xpert MTB/RIF, IGRA, TNF-α, and TGF-βs expression have value in the rapid diagnosis of bone and joint tuberculosis, and the sensitivity and accuracy of bone and joint tuberculosis diagnosis by combining them can improve it.

Cited by 21 publications

References 32 publications

The application of IL-10 and TNF-α in expressed prostatic secretions and prostatic exosomal protein in urine in the diagnosis of patients with chronic prostatitis

The application of IL-10 and TNF-α in expressed prostatic secretions and prostatic exosomal protein in urine in the diagnosis of patients with chronic prostatitis

Tuberculous Extensor Tenosynovitis Presenting as Huge Mass on the Dorsum of the Hand

Diagnostic accuracy of the Xpert MTB/RIF assay for bone and joint tuberculosis: A meta-analysis

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