Application of Morphological and Molecular Systematics for Identification of Green Microalgae of the Genus Chlorococcum and Some Closely Related Taxa

Темралеева, А. Д.; Moslalenko, S. V.

doi:10.1134/s0026261719010077

Cited by 10 publications

(9 citation statements)

References 26 publications

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“…As these results strongly suggest that O. amblystomatis belongs to the genus Chlorococcum, it is here proposed that this species should be renamed as Chlorococcum amblystomatis as given in the tree. Moreover, these results confirm previous data suggesting that the genus Chlorococcum can be clearly divided into two macroclades: Stephanosphaerinia and Moewusinia[31]. ML bootstrap values (>50) and BI posterior probabilities (>0.70) are indicated at the respective branches.…”

supporting

confidence: 89%

“…Interestingly, C. ellipsoideum appears to be an outgroup, branching off these taxa with maximum support. Although this isolate does present similarities to C. infusionum, the holotype of the genus, concerning its reproduction, phylogenetic analysis strongly suggests that belongs to the Stephanosphaerinia macroclade, rather than to the Moewusinia, the macroclade of C. infusionum (Figure 3) [31]. Moreover, upon submitting our sequence to GenBank, the authors found that this sequence is 100% identical to an Oophila amblystomatis (Kingston isolate, Oophila clade A) sequence with the accession number KY091671 [32] and to other sequences that have not been classified down to the species level.…”

Section: Molecular Identification and Phylogenetic Analysismentioning

confidence: 95%

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Isolation, Identification and Biotechnological Applications of a Novel, Robust, Free-living Chlorococcum (Oophila) amblystomatis Strain Isolated from a Local Pond

Correia

Pereira

Silva³

et al. 2020

Applied Sciences

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Bioprospection of novel autochthonous strains is key to the successful industrial-scale production of microalgal biomass. A novel Chlorococcum strain was recently isolated from a pond inside the industrial production facility of Allmicroalgae (Leiria, Portugal). Phylogenetic analysis based on 18S ribosomal ribonucleic acid (rRNA) gene sequences suggests that this isolate is a novel, free-living Oophila amblystomatis strain. However, as our phylogenetic data strongly suggests that the aforementioned taxon belongs to the genus Chlorococcum, it is here proposed to rename this species as Chlorococcum amblystomatis. In order to characterize the biotechnological potential of this novel isolate, growth performance and biochemical composition were evaluated from the pilot (2.5-m3) to industrial (10-m3) scale. The highest maximum areal productivity (36.56 g·m−2·day−1) was reached in a 10-m3 tubular photobioreactor (PBR), as compared to that obtained in a 2.5-m3 PBR (26.75 g·m−2·day−1). Chlorococcum amblystomatis displayed high protein content (48%–56% dry weight (DW)) and moderate levels of total lipids (18%–31% DW), carbohydrates (6%–18% DW) and ashes (9%–16% DW). Furthermore, the lipid profile was dominated by polyunsaturated fatty acids (PUFAs). The highest pigment contents were obtained in the 2.5-m3 PBR, where total chlorophylls accounted for 40.24 mg·g−1 DW, followed by lutein with 5.37 mg·g−1 DW. Overall, this free-living Chlorococcum amblystomatis strain shows great potential for nutritional applications, coupling a promising growth performance with a high protein content as well as relevant amounts of PUFAs, chlorophyll, and carotenoids.

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supporting

confidence: 89%

Section: Molecular Identification and Phylogenetic Analysismentioning

confidence: 95%

Isolation, Identification and Biotechnological Applications of a Novel, Robust, Free-living Chlorococcum (Oophila) amblystomatis Strain Isolated from a Local Pond

Correia

Pereira

Silva³

et al. 2020

Applied Sciences

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“…Molecular phylogenetic analysis. To perform the molecular phylogenetic analysis, a sample of sequences of the 18S rDNA gene with a length of at least 1650 pairs of nucleotides from GenBank (NCBI) corresponding to green algae of the order Chlamydomonadales was made based on the literature data (Temraleeva et al, 2017;Maltsev & Konovalenko, 2017;Mikhailyuk et al, 2018;Temraleeva & Moslalenko, 2019). Cyanoprokaryota such as Geitlerinema splendidum 014 JQ712602.1 (scientific name: Geitlerinema splendidum (Greville ex Gomont) Anagnosti-dis) and Geitlerinema amphibium I013-0021 KY550458.1 (scientific name: Anagnostidinema amphibium (C. Agardh ex Gomont) Strunecký, Bohunická, J. R. Johansen & J. Komárek) were used as an outgroup.…”

Section: Methodsmentioning

confidence: 99%

Halotolerant strain of Chlorococcum oleofaciens from the Lake Elton Biosphere Reserve

2019

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Chlorococcum oleofaciens is one of the most studied representatives of the Chlorococcum genus, both on the ultrastructural and molecular levels. This alga is very interesting due to its ability to hypersynthetize saturated and unsaturated fatty acids and the possibility of using it as a promising object for biofuel production. This research is devoted to the study of the halotolerant strain of Ch. oleofaciens Ch-1 extracted from the water of the Khara River (Lake Elton Biosphere Reserve, Russia, a UNESCO World Heritage site), mineralization of 14‰. The strain Ch. oleofaciens Ch-1 was studied at the morphological level (light microscopy), as well as using molecular genetics methods (18S rDNA). The objectives of the study included establishing the range of halotolerance of the allocated strain of Ch. oleofaciens as a whole, revealing borders of level of mineralization that are optimum for algae growth, and also tracing features of its morphology and cycle of development in the conditions of various salinity. In the course of the studies performed it was established that the extracted strain of Ch. oleofaciens Ch-1 differed from the typical one by greater variability of some morphological features and had a wide ecological valence: the range of its halotolerance was 0–60‰. The maximum values of quantitative development of Ch. oleofaciens Ch-1 were registered at mineralization of 0–14‰. It is shown that with increasing salinity in the development cycle of the strain, the duration of the adaptation phase increases, the exponential phase decreases, small celled forms are replaced by large celled forms and reproduction features are noted. The obtained results can be used for selection of optimal conditions for cultivation of the halotolerant strain of Ch. oleofaciens for biotechnological purposes.

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“…CALU 934 was isolated from the soil samples on the shore of a lake in the Gvardeyskoye Settlement, Leningrad Oblast, European part of Russia [ 90 , 195 ]. The strain defined as Deasonia granata ACKU 566–06 (authentic strain of the genus) was isolated by Pringsheim from soil in the vicinity of the city of Praha (former Czechoslovakia) [ 116 , 117 ], the strain Deasiona garanata ACSSI 150 was isolated from the soil in Gomel (Belarus) [ 196 ] ( Table 2 ).…”

Section: Diversity and Distribution Of Unicellular Carotenogenic Algaementioning

confidence: 99%

Diversity and Distribution of Carotenogenic Algae in Europe: A Review

Chekanov

2023

Marine Drugs

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Microalgae are the richest source of natural carotenoids, which are valuable pigments with a high share of benefits. Often, carotenoid-producing algae inhabit specific biotopes with unfavorable or even extremal conditions. Such biotopes, including alpine snow fields and hypersaline ponds, are widely distributed in Europe. They can serve as a source of new strains for biotechnology. The number of algal species used for obtaining these compounds on an industrial scale is limited. The data on them are poor. Moreover, some of them have been reported in non-English local scientific articles and theses. This review aims to summarize existing data on microalgal species, which are known as potential carotenoid producers in biotechnology. These include Haematococcus and Dunaliella, both well-known to the scientific community, as well as less-elucidated representatives. Their distribution will be covered throughout Europe: from the Greek Mediterranean coast in the south to the snow valleys in Norway in the north, and from the ponds in Amieiro (Portugal) in the west to the saline lakes and mountains in Crimea (Ukraine) in the east. A wide spectrum of algal secondary carotenoids is reviewed: β-carotene, astaxanthin, canthaxanthin, echinenone, adonixanthin, and adonirubin. For convenience, the main concepts of biology of carotenoid-producing algae are briefly explained.

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Application of Morphological and Molecular Systematics for Identification of Green Microalgae of the Genus Chlorococcum and Some Closely Related Taxa

Cited by 10 publications

References 26 publications

Isolation, Identification and Biotechnological Applications of a Novel, Robust, Free-living Chlorococcum (Oophila) amblystomatis Strain Isolated from a Local Pond

Isolation, Identification and Biotechnological Applications of a Novel, Robust, Free-living Chlorococcum (Oophila) amblystomatis Strain Isolated from a Local Pond

Halotolerant strain of Chlorococcum oleofaciens from the Lake Elton Biosphere Reserve

Diversity and Distribution of Carotenogenic Algae in Europe: A Review

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