Application of multiplex-ready PCR for fluorescence-based SSR genotyping in barley and wheat

QTL mapping for brown rot (Monilinia fructigena) resistance in an intraspecific peach (Prunus persica L. Batsch) F1 progeny

“…SSR amplifications were performed following the multiplexready PCR protocol (Hayden et al 2008a;Hayden et al 2008b) with modifications as in Eduardo et al (2010a). Allele size was manually determined using the GeneMarker demo version 1.70 (SoftGenetics).…”

“…In good agreement with previous reports (Eduardo et al 2010b;Forcada et al 2012), we successfully amplified 96.3 % of the tested peach SSRs and 86.3 % other Prunus-derived SSRs. The multiplex-ready strategy used for SSR genotyping allowed screening and mapping of a high number of markers (92.5 % of the 350 screened markers, Table 3) with cost-and time-saving efficiency, as previously described in many crop species, including peach, apricot, and cherry (Hayden et al 2008a;Hayden et al 2008b;Eduardo et al 2010b). Observed polymorphism on peach-derived SSRs was higher than for other Prunus-derived SSRs and higher for genomic SSRs than for cDNA SSRs (Table 3), as previously reported in peach (Dirlewanger et al 2007;Eduardo et al 2010b) and other Rosaceae (Gasic et al 2009;Mnejja et al 2010).…”

Identification of candidate genes at the Dp-fl locus conferring resistance against the rosy apple aphid Dysaphis plantaginea

“…The gel was further stained with the silver staining method and images were acquired using the 440 CF Kodak Image System. SNP markers (SCAR_1 and TSP_57) were scored using the temperature switch PCR (TSP) method (Hayden et al 2008;Tabone et al 2009) according to Pagliarani et al (2016). PCR products were separated in 1.…”

Development and application of microsatellites in plant breeding

“…The methodology of multiplexing can also be carried out in PCR amplification step with mixtures of the appropriate primers (Hayden et al 2008). Current technology allows capillary-based separation of microsatellite bands with four different color panels, one for each individual marker, which can be evaluated through a single capillary with a separate size standard, allowing precise band size estimates (Coburn et al 2002).…”