Application of multipotent mesenchymal stromal cells from human adipose tissue for compensation of neurological deficiency induced by 3-nitropropionic acid in rats

Abstract

We evaluated possible therapeutic effect of multipotent mesenchymal stromal cells from human adipose tissue differentiated to neuronal phenotype with retinoic acid on Wistar rats subjected to toxic effect of 3-nitropropionic acid. Transplantation of mesenchymal stromal cells from human adipose tissue considerably decreased neurological symptoms, normalized exploratory activity (open field test) and long-term memory (Morris test), which correlated with normalization of pathomorphological manifestations in the brain. Destructive changes in the caudate nucleus caused by treatment with 3-nitropropionic acid (reduced size of neurons, changes in their shape, and cell edema) tended to decrease under the effect of multipotent mesenchymal stromal cells: the area of neurons increased 2-fold, the cells acquired typical round shape, cell edema decreased.

Neurogenic differentiation of human adipose-derived stem cells: Relevance of different signaling molecules, transcription factors, and key marker genes

“…But stem cells, with ability to self-renew and differentiate into multiple lineages, are found in different tissues and adipose tissue is an abundant source of adiposederived stem cells (ASCs) (Gronthos et al, 2001;Hauner et al, 1987;Zuk et al, 2001). Human ASCs have capacity to differentiate in vitro into neuron-like cells (Anghileri et al, 2008;Ashjian et al, 2003;Cardozo et al, 2010;Dhar et al, 2007;Jang et al, 2010;Safford et al, 2002), and in vivo may contribute to functional benefits in a wide range of neurological insults (Chi et al, 2010;Kang et al, 2003;Kim et al, 2007;Kulikov et al, 2008;Wei et al, 2009).…”

Adipose-Derived Stem Cells Reduce Lipopolysaccharide-Induced Myelin Degradation and Neuroinflammatory Responses of Glial Cells in Mice

“…ASCs transplantation increases cell proliferation and the number of small vessels, and significantly decreases the area of ischemic brain injury. Human ASCs reduce the destructive changes of neurons in the rat caudate nucleus caused by the injection of 3-nitropropionic acid and decrease edema [ 8 ]. The mechanisms of ASCs effect are not fully understood.…”

Transcriptional Characterization of Wnt and Notch Signaling Pathways in Neuronal Differentiation of Human Adipose Tissue-Derived Stem Cells

“…They have the capacity to differentiate in vitro into mesodermal and nonmesodermal lineages. One of the cell type obtained in vitro was neuron-like cells (Safford et al 2002;Ashjian et al 2003;Dhar et al 2007;Anghileri et al 2008;Cardozo et al 2010;Jang et al 2010); and in vivo may contribute to functional benefits in a wide range of neurological insults (Kang et al 2003;Kim et al 2007;Kulikov et al 2008;Wei et al 2009;Chi et al 2010). This transdifferentiation to nonmesodermal lineages of hASCs is supported by the expression of genes across the three germ layers (Boquest et al 2005;Katz et al 2005;Peroni et al 2008).…”