Application of nano-ELISA in food analysis: Recent advances and challenges

Wu, Long; Guang-hui, LI; Xu, Xiaohong; Zhu, Lin; Huang, Riming; Chen, Xiaoqiang

doi:10.1016/j.trac.2019.02.002

Cited by 250 publications

(114 citation statements)

References 113 publications

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“…On the basis of the antigen‐antibody recognition (Figure Ai‐ii), POD‐ or OXD‐like nanozymes can be used as a signal tag to replace the function of HRP (Figure Aiii‐b), forming a nanozyme‐based ELISA (NLISA) assay. The simple replacement with nanozymes endows NLISA much superiority over traditional ELISA (He et al., ; Wu, Li et al., ). For example, NLISA methods could reach higher reproducibility, because nanozymes could evade the problems of losing activity that are often faced by HRP during labeling or washing steps in the assay.…”

Section: Principles Of Detection Using Enzyme‐mimetic Nanomaterialsmentioning

confidence: 99%

“…On the basis of these facts, positive investigations are devoted to create stimuliresponsive surfaces of nanozymes by surface engineering strategies to allow for not only target recognition, but also target-dependent catalytic activities. With rapid advance of nanotechnology, there are a number of successful examples of nanozyme modulators , including ions (Huang, Zhang et al, 2017;Huang, Zhu et al, 2019), small molecules (Chang, Lin, Xiao, Chiu, & Hu, 2016), nucleotides and nucleic acids (Huang, Chen et al, 2018;Xu, Liu, Wu, Ren, & Qu, 2014;Yang, Wang, & Tseng, 2017), amino acids and peptides (Fan et al, 2017), proteins (Li, Zhang, Wang, Shen, & Hu, 2015), and polymers (Zhang, Zhang, Liu, & Liu, 2017), etc ( Figure 2B). They could function as artificial enzymatic stimulators/inhibitors to adjust the activities of nanozymes, which are generally donated by the changeable surface chemistry of nanomaterials.…”

Section: Nanozyme As Recognition Receptormentioning

confidence: 99%

“…Typical examples of nanozyme-based agrifood detections. Nanozyme-based detection of (A) antioxidants(Jia et al, 2016), (B) glucose(Huang, Zhu et al, 2018), (C) allergens(He et al, 2018), (D) H 2 O 2(Qi et al, 2017), (E) mercury ions(Huang, Zhu et al, 2019), (F) kanamycin(Zeng et al, 2018), (G) pesticides: a and b, (H) mycotoxins: a(Huang, Chen et al, 2018) and b (Molinero-Fernández et al, 2017), and (I) foodborne pathogens.…”

mentioning

confidence: 99%

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Development of Nanozymes for Food Quality and Safety Detection: Principles and Recent Applications

Huang

Sun

et al. 2019

Comp Rev Food Sci Food Safe

146

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The public concerns about agrifood safety call for innovative and reformative analytical techniques to meet the inspection requirements of high sensitivity, specificity, and reproducibility. Enzyme‐mimetic nanomaterials or nanozymes, which combine enzyme‐like properties with nanoscale features, emerge as an excellent tool for quality and safety detection in the agrifood sector, due to not only their robust capacity in detection but also their attraction in future‐oriented exploitations. However, in‐depth understanding about the fundamental principles of nanozymes for food quality and safety detection remains limited, which makes their applications largely empirical. This review provides a comprehensive overview of the principles, designs, and applications of nanozyme‐based detection technique in the agrifood industry. The discussion mainly involves three mimicking types, that is, peroxidase, oxidase, and catalase‐like nanozymes, capable of detecting major agrifood analytes. The current principles and strategies are classified and then discussed in details through discriminating the roles of nanozymes in diverse detection platforms. Thereafter, recent applications of nanozymes in detecting various endogenous ingredients and exogenous contaminants in foods are reviewed, and the outlook of profound developments are explained. Evidenced by the increasing publications, nanozyme‐based detection techniques are narrowing the gap to practical‐oriented food analytical methods, while some challenges in optimization of nanozymes, diversification of recognition‐to‐signal manners, and sustainability of methodology need to conquer in the future.

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Section: Principles Of Detection Using Enzyme‐mimetic Nanomaterialsmentioning

confidence: 99%

Section: Nanozyme As Recognition Receptormentioning

confidence: 99%

mentioning

confidence: 99%

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Development of Nanozymes for Food Quality and Safety Detection: Principles and Recent Applications

Huang

Sun

et al. 2019

Comp Rev Food Sci Food Safe

146

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“…Traditional biomolecule detection techniques are used in a range of fields such as in medicine [1] and the food industry [2], but their use requires many reagents and labeled molecules, as well as specialized equipment and qualified personnel [3,4]. Biosensors are an alternative detection method that overcomes the limitations of traditional detection techniques, allowing real-time monitoring of biological events by transforming them into measurable signals [1].…”

Section: Introductionmentioning

confidence: 99%

“…1 [7]: Δf = − 2 f 0 2 Δm / (ρμ) 1/2 Eq. 1 Where Δf is the frequency shift produced as a consequence of the mass alteration per unit of active area Δm, f 0 is the fundamental resonance frequency, ρ is the quartz density (2648 g/cm 3 ), and μ is the quartz shear modulus (29.47 dynes/cm 2 , for AT-cut) [8].…”

Section: Introductionmentioning

confidence: 99%

Development of a high frequency piezoelectric immunosensor for the detection and quantification of BSA

2020

Biointerface Res Appl Chem

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In this work, we developed a High Fundamental Frequency –Quartz Crystal Microbalance (HFF-QCM) immunosensor for the quantification of Bovine Serum Albumin (BSA) protein. Immobilization of BSA was achieved by means of mixed self-assembled monolayers (SAM), and we found that the largest phase shifts were produced with a concentration of 10 mg/mL. Then, we plotted an anti-BSA vs phase shift calibration curve, and obtained the analytical parameters that allowed us to compare our sensor to another anti–BSA QCM-based sensors. Our HFF-QCM immunosensor displays more sensitivity than low frequency QCM-based biosensors: its limit of detection (LOD) is 100 ng/mL and its linear range extends from 100 ng/mL to 5000 ng/mL. Finally, we fitted different adsorption isotherms models to our experimental data. We chose the Hill isotherm as it presented the highest coefficient of determination (R2), and we determined that the interaction between BSA and anti-BSA displays a positively cooperative binding behavior.

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A Flexible Aptameric Graphene Field‐Effect Nanosensor Capable of Automatic Liquid Collection/Filtering for Cytokine Storm Biomarker Monitoring in Undiluted Sweat

Huang,

Li,

Liu

et al. 2023

Adv Funct Materials

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A wearable sensor capable of directly detecting cytokines in external secretions external to the human body is pivotal for gauging the intensity of the immune response and circumventing inflammatory storms, a significant clinical concern. The detection of low‐concentration cytokines is often hampered due to interference from impurities in secretions. Addressing this challenge, a wearable detection system is developed by integrating a Janus membrane with water auto‐collection/filtering capabilities, and aptamer‐modified graphene field‐effect transistor sensors, enabling the detection of low‐concentration cytokines in undiluted sweat samples. The Janus membrane can automatically filter external secretions and transport them to the sensor surface, effectively eliminating impurity interference and enhancing the sensor's detection capabilities. The device can sensitively detect cytokines (such as TNF‐α, a marker for inflammation and cancer) in undiluted sweat, with a detection range spanning 0.5 to 500 pM, and a detection limit reaching 0.31 pM. The ultra‐flexibility of the sensor is also verified, maintaining stable electrical properties even after enduring up to 50 extreme deformations. These findings indicate that the wearable detection system has the potential to realize low‐concentration cytokines monitoring in the sweat of inflammatory patients, to assist the healthcare system in controlling the body's immune status.

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Application of nano-ELISA in food analysis: Recent advances and challenges

Cited by 250 publications

References 113 publications

Development of Nanozymes for Food Quality and Safety Detection: Principles and Recent Applications

Development of Nanozymes for Food Quality and Safety Detection: Principles and Recent Applications

Development of a high frequency piezoelectric immunosensor for the detection and quantification of BSA

A Flexible Aptameric Graphene Field‐Effect Nanosensor Capable of Automatic Liquid Collection/Filtering for Cytokine Storm Biomarker Monitoring in Undiluted Sweat

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